DOI: https://doi.org/10.14710/dimj.v5i2.24232

Sleeve Gastrectomy Improved TGF-β and IL-10 Expression in Obesity and Diabetes Mellitus Rats

*Sindhu Nugroho Mukti  -  Surgery Resident, Department of Surgery, Faculty of Medicine, Diponegoro University / Dr. Kariadi, Semarang, Indonesia, Indonesia
Sigit Adi Prasetyo  -  Department of Digestive Surgery, Faculty of Medicine, Diponegoro University/Dr. Kariadi, Semarang, Indonesia, Indonesia
Muhammad Ali Shobirin  -  Department of Cardiology and Vascular Medicine, Faculty of Medicine, Diponegoro University/Dr. Kariadi, Semarang, Indonesia, Indonesia
Abdul Mughni Rozy  -  Department of Digestive Surgery, Faculty of Medicine, Diponegoro University/Dr. Kariadi, Semarang, Indonesia, Indonesia
Yan Wisnu Prajoko  -  Department of Surgical Oncology Department, Faculty of Medicine, Diponegoro University/Dr. Kariadi, Semarang, Indonesia, Indonesia
Ismail Bin Sagap  -  Department of General and Colorectal Surgery, Faculty of Medicine, University Kebangsaan Malaysia, Kuala Lumpur, Malaysia
Open Access Copyright (c) 2024 Diponegoro International Medical Journal
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Citation Format:
Abstract

Introduction: Atherosclerosis is a cardiovascular disease which is the leading cause of 16% of the world's total deaths. The main comorbidities of atherosclerosis are obesity and diabetes mellitus. One of obesity surgery treatment that is currently very often used is the Sleeve Gastrectomy. Sleeve Gastrectomy is the most effective and significant long-term therapy for weight loss and preventing various comorbidities such as heart disease. This study analyze the effect of sleeve gastrectomy on the expression of atherosclerosis anti-inflammatory cytokines (TGF-β and IL-10) in the abdominal aorta on the process of atherosclerosis in obesity and diabetes mellitus subjects.


Methods: An in vivo laboratory experimental study with post-test control group design on 15 male rats (Sprague Dawley) that was randomly divided into 3 groups: K1 (negative control), K2 (positive control with obesity and diabetes mellitus) and P1 (obese and DM rats treated with sleeve gastrectomy). After 10th day of the procedure, we analyze the body weight, fasting blood glucose, and gene expression of TGF-β and IL-10 using the PCR method. Statistical analysis was done using Anova, Kruskal Wallis and Post Hoc LSD.


Results: There was significant decrease in body weight (p=0,000) and fasting blood sugar levels (p=0,000) in the P1 compared to the K2. There was significant increase of TGF-β (p = 0,005) and IL-10 (p = 0,002) gene expression in P1 compared to K1 and K2.


Conclusion: SG decreased body weight and fasting blood sugar levels of obese and DM subjects. Also improved the expression of atherosclerosis anti-inflammatory cytokines (TGF-β and IL-10) in abdominal aorta.

Note: This article has supplementary file(s).

Fulltext View|Download |  Research Instrument
Ethical clearance and documentation
Subject
Type Research Instrument
  Download (770KB)    Indexing metadata
Keywords: Obesity; Atherosclerosis; Sleeve gastrectomy; TGF-β; IL-10

Article Metrics:

Article Info
Section: Original Research Article
Language : EN
Recent articles
Gender Identity and Phenotypic Variation of Sex Chromosome Disorders: A Study at Center for Disorder of Sex Development in Indonesia THE EFFECT OF HIIT (HIGH INTENSITY INTERVAL TRAINING) TRAINING ON THE MUSCLE STRENGTH OF THE UPPER BODY OF FK UNDIP STUDENTS Clove Flower Extract (Syzygium aromaticum) enhance Wound Healing Time, Bacterial Count, and IL 10 Levels in Methicillin Resistant Staphylococcus aureus infected Rats More recent articles
  1. Swinburn BA, Sacks G, Hall KD, McPherson K, Finegood DT, Moodie ML et al. The Global Obesity Pandemic: Shaped by Global Drivers and Local Environments. Lancet. 2011;378(9793):804–14
  2. Obesity and Overweight. WHO. 2020
  3. Xihua Lin HL. Obesity: Epidemiology, Pathophysiology, and Therapeutics. Front Endocrinol (Lausanne). 2021;
  4. NCD Risk Factor Collaboration (NCD-RisC). Worldwide Trends in Body-Mass Index, Underweight, Overweight, and Obesity From 1975 to 2016: A Pooled Analysis of 2416 Population-Based Measurement Studies in 128·9 Million Children, Adolescents, and Adults. Lancet. 2017;390(10113)
  5. Badan Penelitian dan Pengembangan Kesehatan. RISKESDAS. Kementerian Kesehatan Republik Indonesia. 2018
  6. Soelistijo SA, Lindarto D, Decroli E, Permana H, Sucipto KW, Kusnadi Y, et al. Pedoman Pengelolaan dan Pencegahan Diabetes Melitus Tipe 2 Dewasa di Indonesia. PB PERKENI; 2019
  7. Bray GA, Heisel WE, Afshin A, Jensen MD, Dietz WH, Long M, et al. The Science of Obesity Management: An Endocrine Society Scientific Statement. Endocr Rev. 2018 Apr;39(2):79–132
  8. Kheirvari M, Dadkhah Nikroo N, Jaafarinejad H, Farsimadan M, Eshghjoo S, Hosseini S, et al. The advantages and disadvantages of sleeve gastrectomy; clinical laboratory to bedside review. Heliyon. 2020 Feb;6(2):e03496
  9. Funes DR, Lo Menzo E, Szomstein S, Rosenthal RJ. Physiological Mechanisms of Bariatric Procedures. In: The ASMBS Textbook of Bariatric Surgery. Cham: Springer International Publishing; 2020. p. 61–76
  10. Wang AW, Cauvi DM, Hawisher D, Reyes T, Coimbra R, Bickler S, et al. The Contribution of the Omentum to the Outcome From Sepsis: An Experimental Animal Study. Shock. 2019 Dec;52(6):604–11
  11. Ambrose JA, Singh M. Pathophysiology of coronary artery disease leading to acute coronary syndromes. F1000Prime Rep. 2015;7(January):1–5
  12. Prud’homme, G. Pathobiology of transforming growth factor β in cancer, fibrosis and immunologic disease, and therapeutic considerations. Lab Invest 87, 1077–1091 (2007). https://doi.org/10.1038/labinvest.3700669
  13. Mirzaalian Y, Nourian M, Gholamalizadeh M, Doaei S, Hatami M, Hassanzadeh A, et al. The association of quantitative insulin sensitivity indices (HOMA-IR and QUICKI) with anthropometric and cardiometabolic indicators in adolescents. Arch Med Sci - Atheroscler Dis. 2019;4(1):32–7
  14. Aristithes G. Doumouras, Jorge A. Wong, J. Michael Paterson, Yung Lee, Branavan Sivapathasundaram, Jean-Eric Tarride, Lehana Thabane, Dennis Hong, Salim Yusuf MA. Bariatric Surgery and Cardiovascular Outcomes in Patients With Obesity and Cardiovascular Disease: A Population-Based Retrospective Cohort Study. Circ AHA. 2021;143:1468–80
  15. Sugondo S. Obesitas. In: Setiati S, Alwi I, Sudoyo AW, Simadibrata M, Setyohadi B, Syam AF, editors. Buku Ajar Ilmu Penyakit Dalam. VI. Jakarta: Interna Publishing; 2016. p. 2561
  16. Hastuti P. Genetika Obesitas. Yogyakarta: Gadjah Mada Universuty Press; 2017
  17. Kumar V, Abbas AK, Aster JC. Buku Ajar Patologi Robbins. 9th ed. Ham M, Saraswati H, editors. Elsevier Inc.; 2015
  18. Oh Niranjan AD. Myocardial infarction. Rev Contemp Pharmacother. 1996;7(1):23–38
  19. Karo, Rohajoe, Sulistyo, Kosasih. Buku Panduan Kursus Bantuan Hidup Jantung Lanjut : ACLS. Jakarta: PERKI; 2012
  20. Mechanic OJ GM. Acute Myocardial Infarction. Treasure Isl. 2021;6(1)
  21. Askarpour M, Khani D, Sheikhi A, Ghaedi E AS. Effect of Bariatric Surgery on Serum Inflammatory Factors of Obesitas Patients : a Systematic Review and Meta-Analysis. Surg Obes Relat Dis. 2019;10:2631–40
  22. Viana EC, Araujo-Dasilio KL, Miguel GPS, Bressan J, Lemos EM, Moyses MR, et al. Gastric bypass and sleeve gastrectomy: The same impact on IL-6 and TNF-α. Prospective clinical trial. Obes Surg. 2013;23(8):1252–61
  23. 23Fang KC, Wolters PJ, Steinhoff M et al. Ekspresi sel mast dari gelatinase A dan B diatur oleh kit-ligan dan TGF-βeta. J Imunol 1999;5528–5535
  24. Frangogiannis NG, Smith CW, Entman ML. The inflammatory response in myocardial infarction. Cardiovasc Res. 2002;53(1):31–47
  25. PERKI. Pedoman Tatalaksana Sindrom Koroner Akut Edisi Ketiga. 2015
  26. Travis, M. A., & Sheppard, D. (2014). TGF-β activation and function in immunity. Annual review of immunology, 32, 51–82. https://doi.org/10.1146/annurev-immunol-032713-120257
  27. Fauna J, Karangmalang N. Peranan TGF β-1 dalam Perkembangan Penyakit Jantung Akibat Induksi Diet Lemak Tinggi.pdf. 2012;30(1):8–13
  28. Sadler, CH, LI Rutitzky, MJ Stadecker, dan RA Wilson. 2003. IL-10 sangat penting untuk transisi dari keadaan penyakit akut ke kronis selama infeksi tikus dengan Schistosoma mansoni. eur. J. Imun. 33: 880 – 888
  29. Anderson, C. F., M. Oukka, V. J. Kuchroo, and D. Sacks. 2007. CD4,CD25, Th1 cells are the source of IL-10-mediated immune suppres- sion in chronic cutaneous leishmaniasis. J. Exp. Med. 204: 285–297
  30. Boonstra,A.,R.Rajsbaum,M.Holman,R.Marques,C.Asselin-Paturel,J.P.Pereira, E. E. Bates, S. Akira, P. Vieira, Y. J. Liu, et al. 2006. Macrophages and myeloid dendritic cells, but not plasmacytoid dendritic cells, produce IL-10 in response to MyD88- and TRI
  31. Edwards, J. P., X. Zhang, K. A. Frauwirth, and D. M. Mosser. 2006. Biochemical and functional characterization of three activated macrophage populations. J. Leukocyte Biol. 80: 1298 –1307
  32. Katakura, T., M. Miyazaki, M. Kobayashi, D. N. Herndon, and F. Suzuki. 2004. CCL17 and IL-10 as effectors that enable alternatively activated macrophages to inhibit the generation of classically activated macrophages. J. Immunol. 172: 1407–1413
  33. Couper KN, Blount DG, Riley EM. IL-10: The Master Regulator of Immunity to Infection. J Immunol. 2008;180(9):5771–7
  34. Frangogiannis NG, Smith CW, Entman ML. Respon inflamasi pada infark miokard. 2002;53:31–47
  35. Ross R. Atherosclerosis—an inflammatory disease. 1999;340:115–26
  36. Alfred Wirth, Martin Wabitsch HH. The prevention and treatment of obesity. Dtsch Arztebl Int. 2014;111(42)
  37. Major P, Wysocki M, Pedziwiatr M, Malczak P, Pisarska M, Budzynski A. Laparoscopic sleeve gastrectomy for the treatment of diabetes mellitus type 2 patients-Single center early experience. Gland Surg. 2016;5(5):465–72
  38. Dimas E, Mughni A, Wiryawan C, Mahendra V, Riwanto I. Sleeve gastrectomy and pancreas omentoplasty improved β cell insulin expression and interleukin-1β serum level in non-Obesitas diabetes mellitus rat. Bali Med J. 2021;10(1):460–6
  39. Global BMI Mortality Collaboration, Di Angelantonio E, Bhupathiraju SN, Wormser D, Gao P, Kaptoge S, et al. Body-mass index and all-cause mortality: Downloaded from https://academic.oup.com/eurheartj/advance-article/doi/10.1093/eurheartj/ehac071/6542137 b
  40. Doumouras AG, Wong JA, Paterson JM, Lee Y, Sivapathasundaram B, Tarride J-E, et al. Bariatric Surgery and Cardiovascular Outcomes in Patients With Obesity and Cardiovascular Disease: Vol. 143, Circulation. 2021. p. 1468–80
  41. Vest AR, Heneghan HM, Agarwal S, Schauer PR, Young JB. Bariatric surgery and cardiovascular outcomes: A systematic review. Heart. 2012;98(24):1763–77
  42. Kuno T, Tanimoto E, Morita S, Shimada YJ. Effects of Bariatric Surgery on Cardiovascular Disease: A Concise Update of Recent Advances. Front Cardiovasc Med. 2019;6(July)
  43. Zundel N, Hernandez R. JD, Gagner M. Laparoscopic Sleeve Gastrectomy: Technique and Outcomes. In: The ASMBS Textbook of Bariatric Surgery. Cham: Springer International Publishing; 2020. p. 149–59
  44. Chousleb E, Rodriguez JA, O’Leary JP. History of the Development of Metabolic/Bariatric Surgery. In: The ASMBS Textbook of Bariatric Surgery. Cham: Springer International Publishing; 2020. p. 37–46
  45. DuCoin C, Moore RL, Provost DA. Indications and Contraindications for Bariatric Surgery. In: The ASMBS Textbook of Bariatric Surgery. Cham: Springer International Publishing; 2020. p. 77–81
  46. Pucci A, Batterham RL. Mechanisms underlying the weight loss effects of RYGB and SG: similar, yet different. J Endocrinol Invest. 2019 Feb;42(2):117–28
  47. Hagman DK, Larson I, Kuzma JN, Cromer G, Makar K, Rubinow KB, et al. The short-term and long-term effects of bariatric/metabolic surgery on subcutaneous adipose tissue inflammation in humans. Metabolism. 2017 May;70:12–22
  48. Di Nicola V. Omentum a powerful biological source in regenerative surgery. Regen Ther. 2019 Dec;11:182–91
  49. Meza-Perez S, Randall TD. Immunological Functions of the Omentum. Trends Immunol. 2017 Jul;38(7):526–36
  50. Bartholomeus K, Jacobs-Tulleneers-Thevissen D, Shouyue S, Suenens K, In’t Veld PA, Pipeleers-Marichal M, et al. Omentum Is Better Site Than Kidney Capsule for Growth, Differentiation, and Vascularization of Immature Porcine β-Cell Implants in Immunodeficient Rats. Transplantation. 2013 Dec;96(12):1026–33
  51. Huber SA, Sakkinen P, Conze D, Hardin N TR. nterleukin-6 exacerbates early atherosclerosis in mice. rterioscler Thromb Vasc Biol. 1999;
  52. Brethauer SA, Hammel JP, Schauer PR. Systematic review of sleeve gastrectomy as staging and primary bariatric procedure. Surg Obes Relat Dis Off J Am Soc Bariatr Surg. 2009;5(4):469–75
  53. Khanna D, Khanna S, Khanna P, Kahar P, Patel BM. Obesity: A Chronic Low-Grade Inflammation and Its Markers. Cureus. 2022;14(2)
  54. Huang R, Ding X, Fu H, Cai Q. Potential mechanisms of sleeve gastrectomy for reducing weight and improving metabolism in patients with obesity.Surg Obes Relat Dis Off J Am Soc Bariatr Surg. 2019 Oct;15(10):1861–71
  55. Li S, Dong S, Xu Q, Shi B, Li L, Zhang W, et al. Sleeve Gastrectomy-Induced AMPK Activation Attenuates Diabetic Cardiomyopathy by Maintaining Mitochondrial Homeostasis via NR4A1 Suppression in Rats. Front Physiol. 2022;13:837798
  56. Kawasaki T, Ohta M, Kawano Y, Masuda T, Gotoh K, Inomata M, et al. Effects of sleeve gastrectomy and gastric banding on the hypothalamic feeding center in an obese rat model. Surg Today. 2015 Dec;45(12):1560–6
  57. Shen Y, Liu Y, Zheng S-Q, Han J, Pei E-L, Li Z-H, et al. Effects of Left Gastric Artery Ligation Versus Sleeve Gastrectomy on Obesity-Induced Adipose Tissue Macrophage Infiltration and Inflammation in Diet-Induced Obese Rats. Med Sci Monit Int Med J Exp Clin Res. 2019 Sep;25:6719–26
  58. Ahn CH, Choi EH, Lee H, Lee W, Kim J-I, Cho YM. Vertical sleeve gastrectomy induces distinctive transcriptomic responses in liver, fat and muscle. Sci Rep. 2021 Jan;11(1):2310
  59. Aljada A, Ghanim H, Saadeh R, Dandona P. Insulin Inhibits NFκB and MCP-1 Expression in Human Aortic Endothelial Cells. J Clin Endocrinol Metab. 2001 Jan 1;86:450–3
  60. Wu H, Ballantyne CM. Metabolic Inflammation and Insulin Resistance in Obesity. Circ Res. 2020;1549–64

Last update:

No citation recorded.

Last update:

No citation recorded.