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Distribusi Nrf2 dan Enzim GST pada Sel Granulosa Ovarium Tikus Putih Premenopause setelah Pemberian Serbuk Rimpang Kunyit

1Program Studi Biologi Fakultas Sains dan Matematika Universitas Diponegoro, Indonesia

2Program Doktor Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Brawijaya, Indonesia

Open Access Copyright 2021 Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology)

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Abstract

Kurkumin pada tanaman kunyit dapat mengaktivasi agen transkripsi yaitu Nuclear related factor 2 (Nrf2). Nrf2 berperan dalam transkripsi kelompok enzim detosifikasi fase II, seperti glutathione S-transferase (GST). Enzim GST dapat berfungsi sebagai antioksidan dengan melindungi sel dari radikal bebas terutama dari kelompok ROS. Penelitian bertujuan mengetahui distribusi Nrf2 dan enzim GST pada sel granulosa ovarium tikus putih premenopause, setelah pemberian serbuk rimpang kunyit. Hewan uji yang digunakan adalah 30 ekor tikus putih betina, strain wistar, usia 12 bulan, dengan bobot 200-250 g. Enam kelompok perlakuan, yaitu P0; kontrol negatif, diberi akuades 4 ml, P1; kontrol positif, diberi serbuk kurkumin dosis 6,75 mg/kg BB, P2; diberi serbuk rimpang kunyit dosis 100 mg/kg BB, P3; diberi serbuk rimpang kunyit dosis 200 mg/kg BB, P4; diberi serbuk rimpang kunyit dosis 400 mg/kg BB, dan P5; diberi serbuk rimpang kunyit dosis 800 mg/kg BB. Perlakuan per oral setiap hari selama 27 hari. Penelitian menggunakan Rancangan Acak Lengkap, data distribusi Nrf2 dan GST dianalisis menggunakan ANOVA dan dilanjutkan dengan BNJ. Hasil penelitian menunjukkan bahwa pemberian serbuk rimpang kunyit dosis 200mg/kg BB, merupakan dosis optimal dalam meningkatkan distribusi Nrf2 dan enzim GST pada sel granulosa ovarium tikus putih premenopause.

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Keywords: radikal bebas; agen transkripsi; enzim detoksifikasi fase II; kurkumin
Funding: Direktorat Jenderal, Pendidikan Tinggi. Kementerian Riset, Teknologi dan Pendidikan Tinggi, Republik Indonesia.

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  1. Agarwal, A., A. Aponte-Mellado, B.J. Premkumar, A. Shaman, S. Gupta. 2012. The effects of oxidative stress on female reproduction: a review. Reproductive Biology and Endocrinology. 10:1-49
  2. Boenisch, T. 2001. Immunohistochemical Handbook; Antibodies. 3rd edition. DAKO Corporation. California
  3. Bruns, D.R., J.C. Drake, L.M. Biela. 2015. Nrf2 signaling and the slowed aging phenotype: evidence from long-lived models. Oxidative Medicine and Cellular Longevity. 16(5)1-15
  4. Chattopadhyay, I., K. Biswas, U. Bandyopadhyay, R. K. Banerjee. 2004. Turmeric and curcumin: Biological actions and medicinal applications. Current Science. 87:44-50
  5. Doshi, S.B., A. Agarwal. 2014. The role of oxidative stress in menopause. Journal of Mid-life Health. 4(3):140-147
  6. Halliwell, B., J. M. C. Gutteridge. 2015. Free Radical in Biology Medicine 5th Edition (preview). Oxford University Press. Oxford. United Kingdom
  7. Homma, S., Y. Ishii, Y. Morishima, T. Yamadori, Y. Matsuno. 2009. Nrf2 Enhances cell proliferation and resistance to anticancer drugs in human lung cancer. Clinical Cancer Research.15(10):3423-3434
  8. Itoh, K., N. Wakabayashi, Y. Katoh, T. Ishii, K. Igarashi, J.D. Engel, M. Yamamoto. 1999. Keap1 represses nuclear activation of antioxidant responsive elements by Nrf2 through binding to the amino-terminal Neh2 domain. Genes and Development. 13:76-86
  9. Kitani, K., T. Osawa, T. Yokozawa. 2007. The effects of tetrahydrocurcumin and green tea polyphenol on the survival of male C57BL/6 mice. Biogerontology. 8:567-573
  10. Kunwar, A., K.I. Priyadarsini. 2011. Free radicals, oxidative stress and importance of antioxidants in human health. Journal of Medical and Allied Sciences, 1(2):53-60
  11. Li, S., W. Yuan, G. Deng, P. Wang, P. Yang, B.B. Aggarwal, 2011, Chemical composition and product quality control of turmeric (Curcuma longa L.), Pharmaceuti. Crops, 2:28-54
  12. Li, Q., X.D. Geng, W. Zheng, J. Tang, B. Xu, Q.H. Shi. 2012. Current understanding of ovarian aging. Scie. Chi. 55 (8):659-669
  13. Lokhande, S.M., R.V. Kale, A.K. Sahoo, R.C. Ranveer. 2013. Effect of curing and drying methods on recovery, curcumin and essential oil content of different cultivars of turmeric (Curcuma longa L.). International Food Research Journal. 20(2):745-749
  14. Meizarini, A., Siswandono, W. Riawan, R.P. Rahayu. 2018. In Silico and In Vivo anti-inflamatory studies of curcuminoids, turmeric extract with zinc oxide, and eugenol. Tropical Journal of Pharmaceutical Research. 17(2):269-275
  15. Paladino, S. A. Conte, R. Caggiano, G.M. Pierantoni, R. Faraonio. 2018. Nrf2 pathway in age-related neurological disorder: insights into microRNAs. Cellular Physiology and Biochemistry.47:1951-1976
  16. Reyes, S.G., S.G. Beltrán., O.N. Campos, J.P. Chaverri. 2013. Curcumin Pretreatment Induces Nrf2 and an Antioxidant Response and Prevents Hemin-Induced Toxicity in Primary Cultures of Cerebellar Granule Neurons of Rats. Hindawi Publishing Corporation Oxidative Medicine and Cellular Longevity. http://dx.doi.org/10.1155/2013/801418
  17. Sikora, E., G. Scapagnini, M. Barbagallo. 2010 Curcumin, inflammation, ageing and age-related diseases. Immunity Ageing. 7:1-4
  18. Subrat, P., S.A. Santa, J. Vandana. 2013. The Concepts and Consequences of Early Ovarian Ageing: A Caveat to Women’s Health. Journal of Reproduction and Infertility. 14(1):3-7
  19. Suntoro, H. 1983. Metode Pewarnaan (Histologi dan Histokimia). Penerbit Bharata Karya Aksara. Jakarta
  20. Suprihatin, T., S. Widyarti, M. Rifa’i, S. Rahayu. 2020. Pengaruh Pemberian Serbuk Rimpang Kunyit (Curcuma longa L.) terhadap Jumlah Folikel Ovarium Tikus Putih (Rattus norvegicus) Premenopause. Buletin Anatomi dan Fisiologi. 5(2):45-52. https://doi.org/10.14710/baf.5.2.2020.%25p
  21. Suzuki, T., M. Yamamoto. 2015. Molecular basis of the Keap1-Nrf2 system. Free Radical Biology and Medicine. 88(part B):93-100
  22. Tatone, C., F. Amicarelli. 2012. The aging ovary-the poor granulosa cell. Fertility and Sterility. 99(1):12-17
  23. Toden, S & A. Goel. 2017. The holy grail of curcumin and its efficacy in various diseases: is bioavailability truly a big concern?. Journal of Restorative Medicine. 6:27-36
  24. Velasquez J.T., M.E. Watts, M. Todorovic, L. Nazareth. 2014. Low-dose curcumin stimulates proliferation, migration and phagocytic activity of olfactory ensheathing cells. Plos One 9(10):1-17
  25. Zhang, D.D. 2006. Mechanistic studies of the nrf2-keap1 signaling pathway. Drug Metabolism Reviews. 38(4):769-789
  26. Zhuang, C., Z. Wu, C. Xing, Z. Miao. 2017. Small molecules inhibiting Keap1-Nrf2 protein-protein interactions: a novel approach to activate Nfr2 function. Medicinal Chemistry Communication. 8:286-294

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