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Distribusi Nrf2 dan Enzim GST pada Sel Granulosa Ovarium Tikus Putih Premenopause setelah Pemberian Serbuk Rimpang Kunyit

*Teguh Suprihatin orcid scopus  -  Program Studi Biologi Fakultas Sains dan Matematika Universitas Diponegoro, Indonesia
Sri Rahayu  -  Program Doktor Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Brawijaya, Indonesia
Muhaimin Rifa'i  -  Program Doktor Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Brawijaya, Indonesia
Sri Widyarti  -  Program Doktor Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Brawijaya, Indonesia
Open Access Copyright 2021 Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology)

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Abstract

Kurkumin pada tanaman kunyit dapat mengaktivasi agen transkripsi yaitu Nuclear related factor 2 (Nrf2). Nrf2 berperan dalam transkripsi kelompok enzim detosifikasi fase II, seperti glutathione S-transferase (GST). Enzim GST dapat berfungsi sebagai antioksidan dengan melindungi sel dari radikal bebas terutama dari kelompok ROS. Penelitian bertujuan mengetahui distribusi Nrf2 dan enzim GST pada sel granulosa ovarium tikus putih premenopause, setelah pemberian serbuk rimpang kunyit. Hewan uji yang digunakan adalah 30 ekor tikus putih betina, strain wistar, usia 12 bulan, dengan bobot 200-250 g. Enam kelompok perlakuan, yaitu P0; kontrol negatif, diberi akuades 4 ml, P1; kontrol positif, diberi serbuk kurkumin dosis 6,75 mg/kg BB, P2; diberi serbuk rimpang kunyit dosis 100 mg/kg BB, P3; diberi serbuk rimpang kunyit dosis 200 mg/kg BB, P4; diberi serbuk rimpang kunyit dosis 400 mg/kg BB, dan P5; diberi serbuk rimpang kunyit dosis 800 mg/kg BB. Perlakuan per oral setiap hari selama 27 hari. Penelitian menggunakan Rancangan Acak Lengkap, data distribusi Nrf2 dan GST dianalisis menggunakan ANOVA dan dilanjutkan dengan BNJ. Hasil penelitian menunjukkan bahwa pemberian serbuk rimpang kunyit dosis 200mg/kg BB, merupakan dosis optimal dalam meningkatkan distribusi Nrf2 dan enzim GST pada sel granulosa ovarium tikus putih premenopause.

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Keywords: radikal bebas; agen transkripsi; enzim detoksifikasi fase II; kurkumin
Funding: Direktorat Jenderal, Pendidikan Tinggi. Kementerian Riset, Teknologi dan Pendidikan Tinggi, Republik Indonesia.

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