PENAMBAHAN KANDIDAT PROBIOTIK Bacillus methylothrophicus SECARA BERKALA PADA MEDIA PEMELIHARAAN UNTUK PENCEGAHAN INFEKSI BAKTERI Aeromonas hydrophila PADA IKAN NILA (Oreochromis niloticus)

Femy Musthofa Ardy, Desrina Desrina, Alfabetian Harjuno Condro Haditomo

Abstract

Aeromonas hydrophila is a bacteria that causes of MAS disease (motile aeromonad septicemia) in freshwater fish cultivation and can cause mass death in a fairly short period of time in some species including tilapia. There are several alternative strategies in prevention, one of which is the use of probiotic bacteria as agents for controlling or preventing this disease. One candidate for probiotics that has been molecularly identified as 16sRNA and is known to have the ability to inhibit pathogenic bacteria is B. methylotrhrophicus. The aim of this research was to study B.methylotrophicus in inhibiting  A. hydrophila in Oreochromis niloticus culture. This research consisted of in vitro and in vivo test that used experiment method with completely randomized design with 4 treatments (density of 1 fishes/l) and 3 replications. The treatment consisted of a mixture of A. hydrophila 102 CFU/mL with B. methylotrophicus 109 CFU/mL (a) without addition of B. methylotrophicus (b) Addition every 3 days, (c) Addition every 5 days, (d) Addition every 7 day. 120 fishes at average weight of 17,5±1,9 g was used as experimental animals. Based on the in vitro test, the most powerful concentration of B. methylotrophicus to inhibit A. hydrophila was 109 cfu/mL with clear zone of 24,9±4,2 mm. In vivo tests show that the addition of B. methylotrophicus periodecally does not significantly affect survival rates, but can slow the gowth of A. hydrophila. Treatment D showed the highest survival rate (13.33%), followed by treatment A (6.66%), B (3.33%), and C (3.33%). These results indicate that B.methylotrophicus can prevent the gowth of A. hydrophila in vitro, and can increase SR by 6.66% in the in vivo test.

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References

Afnidar. 2014. Fitokimia dan Uji Aktivitas Antibakteri Ekstrak Kalus Tumbuhan Sernai (Wedelia biflora (L)Dc.). J. JESBIO. 3(4), ISSN: 2302-1705.

Agustina,P. A.H.C, Haditomo. Sarjito. 2018. Penambahan Bakteri Kandidat Probiotik (Bacillus methylothrophicus) Dengan Konsentrasi Berbeda Pada Media Budidaya Ikan Nila (Oreochromis niloticus) Untuk Pencegahan Serangan Aeromonas hydrophila. [SKRIPSI]. Universitas Diponegoro. 85 hlm.

Andriani, R. 2016. Pengenalan Alat-Alat Laboratorium Mikrobiologi Untuk Mengatasi Keselamatan Kerja dan Keberhasilan Praktikum. J Mikrobiologi 1(1) ISSN : O1A114084.

Balcazar JL, de-Blas I, Ruiz-Zarzuela I, Vendrell D, Muzquiz JL. 2004. Probiotics: a tool for the future of fish and shellfish health management. J. of Aquaculture in the Tropics. 19: 239–242.

Chopra A, K. X, J, Xu. D, Ribardo. M, Gonzalez. K, Kuhl. J, W, Peterson. C, W, Houston. 2000. The Cytotoxic Enterotoxin of Aeromonas hydrophila Induces Proinflammatory Cytokine Production and Activates Arachidonic Acid Metabolism in Macrophages. J. Infection and Immunity. 68(5). Page : 2808–2818.

Das,S. K, Mondal. Salma, H. 2017. A review on application of probiotic, prebiotic and synbiotic for sustainable development of aquaculture. J of Entomology and ZoologyStudies. 5(2): 422-429.

Desriac, F., Defer, D., Bourgougnon, N., Brillet, B., Chevalier, P.L., Fleury, Y., 2010. Bacteriocin as Weapons in the Marine Animal-Associated Bacteria Warfare: Inventory and Potential Applications as an Aquaculture Probiotic. Mar. Drugs, 8: 1153–1177.

Esteban,M,A. H. Cordero. M, M,Tom. A,M, J,Monreal. A, Bakhrouf. A, Mahdhi. 2014. Effect of dietary supplementation of probiotics and palm fruits extracts on the antioxidant enzyme gene expression in the mucosae of gilthead seabream (Sparus aurata L.). J Fish & Shellfish Imunology :1-9.

Farias, T.H.V., Levy-Pereira, N., L.D.O. Alves, D.D.C. Dias, L. Tachibana, F. Pilarski, M.A.D.A. Belo, M.J.T.Ranzani-Paiva. 2016. Probiotic Feeding Improves The Immunity of Pacus, Piaractusmesopotamicus, during Aeromonas hydrophila Infection. Animal Feed Science and Technology, 211: 137–144.

Garde, C, Thomas. B, M. Givskov, T. H. Jakobsen, M. Hentzer, A. Claussen, Kim. S, Jesper. F, T. Sams. 2010. Quorum Sensing Regulation in Aeromonas hydrophila. J. Mol. Biol. 396 : 49–857. doi:10.1016/j.jmb.2010.01.002.

Haditomo, A.H.C., A.M. Lusiastuti, Widanarni. 2016. Studi Bacillus Firmus Sebagai Kandidat Probiotik Dalam Menghadapi Aeromonas hydrophila PADA Media Budidaya. J Saintek Perikanan, 11 (2): 111-114.

Haditomo, A.H.C., Sarjito, Desrina, S.B. Praytitno, 2017. 10th Symposium On Disease in Asian Aquaculture (DAA 10), 28 Agustus 2017-1 September 2017, Bali, Indonesia. Screening of Isolated Potential Probiotic from Mud Aquaculture in Central Java Indonesia with Molecular Based.

Hardi, E, H. Catur A, P. Triesna H. Rizki T, H. 2014. Infeksi Aeromonas hydrophila Melalui Jalur yang Berbeda Pada Ikan Nila (Oreochromis niloticus) Di Loa Kulu Kutai Kartanegara Kalimantan Timur. J. Kedokteran Hewan. 8 (2), ISSN : 1978 – 225X.

Huang, C.H., T. Ano, and M. Shoda. 1993. Nucleotide sequence and characteristics of the gene, lpa-14, responsible for biosynthesis of the lipopeptide antibiotics iturin A and surfactin from Bacillus subtilis RB14. J of Fermentation and Bioengineering 76: 445-450.

Jemil, N., A.Manresa, F.Rabanal, H.B.Ayed, N.Hmidet, M.Nasri. 2017. Structural Characterization And Identification Of Cyclic Lipopeptides Produced by Bacillus methylotrophicus DCS1 strain. J of Chromatography B, 1060: 374-386.

Kementerian Kelautan dan Perikanan. 2013. Statistik Menakar Target Ikan Air Tawar. Direktorat Jenderal Perikanan Budidaya. Jakarta.

Lang, S. & F. Wagner. 1993. Bioconversion of Oils and Sugars to Glycolipids. Dalam Kosaric N, editor. Biosurfactans. Production. Properties. Application. Marcel Dekker. New York. Hlm 346-382.

Murilio I., L. Villamil. 2011. Bacillus cereus and Bacillus subtilis Used as Probiotics in Rotifer Branchionus plicatilis Cultures. J of Aquaculture Research and Development,07: 1–5.

Nikoskelainen, S., Ouwehand, A., Salminen, S., & Bylund, G. 2001. Protection of Rainbow Trout Onchorhyncus mykiss from furunculosis by Lactobacillus rhamnosus. Aquaculture, 198: 229-236.

Ngoc, N, N. Nguyen V, C. Tran L, H. Pham T, T, G. 2016. Microbiologycal Characterization and Potential Application of Indigenous B. methylotrophycus Ba1 in Handling of Canna edulis. Ker Processing Craft Village Wastewater. J Of Forest Science and Technology No. 5. Page: 4-9.

Olga. 2012. Patogenisitas Bakteri Aeromonas hydrophila Asb01 Pada Ikan Gabus (Ophicephalus striatus). J Sains Akuatik 14 (1): 33 – 39

Sansawat A, Thirabunyanon M. 2009. Anti- Aeromonas hydrophila Activity and Characterisation of Novel Probiotics Strains of Bacillus subtilis Isolated from the Gastrointestinal tract of Giant Freshwater Prawns. Maejo International J of Science and Technology, 3: 77–87.

Sorokulova, I. B. Iryna V, P. Muriel D. Irina G, O. Jen M, H. Simon M, C. Maria C. Urdaci. 2007. The Safety Of Two Bacillus Probiotic Strains For Human Use. J. Dig Dis Sci. 53:954–963. DOI 10.1007/s10620-007-9959-1.

Sharma S.C.D., M.S.Shovon, M.G.S. Jahan, A.K.M. Asaduzzaman, Md. A.Rahman, K.K. Biswas, N. Abe, N.Roy. 2013. Antibacterial And Cytotoxic Activity of Bacillus methylotrophicus-SCS2012 Isolated From Soil. J of Microbiology, Biotechnology and Food Sciences, 2 (4): 2293-2307.

Sya’bani, N. Ayi, Y. Ike, R. A, M, Lusiastuti. 2015. Frekuensi Penambahan Probiotik Bacillus sp dan Staphylococcus sp pada Media Pemeliharaan Benih Ikan Lele Dumbo (Clarias gariepinus) untuk Ketahanan Terhadap Aeromonas hydrophila. J, Perikanan Kelautan. 6(2). Hal : 130-140.

SNI 7550. 2009. Produksi ikan Nila (Oreochromis niloticus Bleeker) kelas Pembesaran di Kolam Air Tenang. 5 hlm.

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