Purifikasi asam 5-aminolevulinat dari kultur bakteri strain B10 dengan ion exchange

Abstract

5-aminolevulinic acid (5-ALA) is an intermediate precursor for the biosynthesis of tetrapyrrol compounds which are widely applied in agriculture. Purification needs to be done since the impurity components are still obtained during production by fermentation. The aim of this research is to detect 5-ALA compounds after purification by ion exchange techniques, and to differentiate the 5-ALA concentrations in the ion exchange purified eluate. Research methods include subculture of bacterial isolates of strain B10, analysis of bacterial growth and detection of 5-ALA, pH measurement, 5-ALA fermentation, 5-ALA crude production, ion exchange purification, 5-ALA analysis with TLC test and UV-Vis spectrophotometer (553 nm), as well as data analysis. This study used a single factor that distinguishes 5-ALA levels after three times elution by ion exchange techniques using the Paired Sample T-test. The results of the study showed that the TLC test had a pink stain, indicated the 5-ALA contained. The test results with a UV-Vis spectrophotometer showed the highest 5-ALA concentration in the second eluate. The results of the analysis using the Paired Sample T-test, exhibited no significant difference (Sig.>,0.05) from the three eluate results of 5-ALA purification. Results from research shows that the product of 5-ALA fermentation of bacterial culture strain B10 can be purified by ion exchange techniques.