Isolasi kapang endofit dari tanaman ciplukan (Physalis angulata L.) dan potensi antibakteri terhadap Escherichia coli dan Staphylococcus aureus

Abstract

Ciplukan (Physalis angulata) is a one of medicinal plant that can be used as antimicrobial because they contain bioactive compound that can inhibit microbial growth. Ciplukan can also be used as source of endophytic mold isolates which can be developed as an alternative for producing antimicrobial compound. This study aims to isolate and characterize the endophytic molds present in ciplukan plants and to select mold isolates that have the potential to inhibit the growth of E. coli and S. aureus bacteria. This study used a completely randomized design (CRD). Ten isolates of mold that have been isolated from ciplukan are from the genus Curvularia, Penicillium, Aspergillus, and several cultures genus is unkown. The obtained mold supernatant was then tested on the tested bacteria using the Kirby-Baurer Method . The results showed that 8 isolates were able to inhibit the growth of E. coli and as many as 5 isolates were able to inhibit the growth of S. aureus. The anova test results showed that each supernatant had a significant difference (p<0,5).