Isolasi bakteri endofit asal tumbuhan mangrove Avicennia marina dan kemampuannya sebagai antimikroba patogen Staphylococcus aureus dan Salmonella typhi secara in vitro

Abstract

Avicennia marina is a mangrove species that has potential as an antimicrobial source. The potential of plants as antimicrobials is not only through bioactive compounds, but also through the endophytic microbes that live in them. This study aims to isolate endophytic bacteria from mangrove Avicennia marina and determine their ability as an antimicrobial against pathogenic bacteria Staphylococcus aureus and Salmonella typhi in in vitrocondition. The sample of Avicennia marina used in this study collected from the Mangkang Mangrove Forest area of Semarang, the parts taken were the roots, stems and leaves. The research methods included isolation of endophytic bacteria, characterization of endophytic bacteria, preparation of tested bacterial cultures, test for antimicrobial activity of endophytic bacteria supernatant and ethyl acetate extract, and data analysis. This study used a Completely Randomized design, data were analyzed using one-way ANOVA and Duncan's test. The results of endophytic bacteria isolation showed that 3 endophytic bacterial isolates were obtained, namely AM1, AM2, and AM3, which morphologically presumed belongs to the genus Pseudomonas sp., Enterobacter sp., and Staphylococcus sp. respectively. The results of the antimicrobial activity test showed that the antimicrobial activity of endophytic bacteria in Salmonella typhi tended to be greater than in Staphylococcus aureus. The results also showed that ethyl acetate extract did not increase the formed inhibition zone.