Verifikasi Penentuan Kadar Sitronelal dalam Minyak  Sereh Wangi Menggunakan GCFID

Surani Surani; Renardi Erwinsyah

doi:10.14710/jplp.8.2.%p

DOI: https://doi.org/10.14710/jplp.8.2.%25p

Verifikasi Penentuan Kadar Sitronelal dalam Minyak Sereh Wangi Menggunakan GCFID

Surani Surani¹ , Renardi Erwinsyah²

¹Program Studi Kima, Universitas Pendidikan Indonesia, Jl. Dr. Setiabudhi No. 229 Bandung, Indonesia

²Program Studi Biologi Universitas Pendidikan Indonesia Jl. Dr. Setiabudhi No. 229 Bandung, Indonesia

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

BibTex Citation Data :

@article{JPLP29947,
    author = {Surani Surani and Renardi Erwinsyah},
    title = {Verifikasi Penentuan Kadar Sitronelal dalam Minyak  Sereh Wangi Menggunakan GCFID},
    journal = {Jurnal Pengelolaan Laboratorium Pendidikan},
  volume = {8},
    number = {2},
    year = {2026},
    keywords = {method validation, citronella, citronellal, GC-FID},
    abstract = {  Citronella oil is one of the essential oils, where the main component is Citronellal. The Instrumental Chemistry Laboratory is a testing laboratory that accepts citronella oil testing samples from both Chemistry Study Program students at the Indonesian University of Education and external students. As a testing laboratory, the analysis must use proven methods, procedures, and equipment to ensure the suitability and purpose of the test. Currently, the Instrumental Chemistry Laboratory will propose and implement ISO/IEC 17025 so that the test results are more valid, consistent, and accountable. The purpose of this study was to verify the method for determining citronellal levels in citronella oil using GC-FID, where the samples used were samples from the isolation and synthesis of organic compounds practicum by chemistry study program students. The results of the study for the linearity of the citronellal standard obtained a correlation value of 0.9988 and has entered the linearity quality requirement of 0.9950. From the results of the accuracy test measurements, the recovery value was 91.15% and entered the quality requirement of 80-110%. From the results of the precision test measurements, the RSD value was 1.87%, which was within the quality requirements of <2%. The limit of detection and limit of quantitation were determined with LOD results of 2.72% and LOQ of 9.06%. Based on the results of the verification study, the method for analyzing citronellal levels in citronella oil using GC-FID has met the acceptance requirements, so it can be concluded that this method has been verified  },
   issn = {2654-251X},   pages = {85--93}  doi = {10.14710/jplp.8.2.%p},
    url = {https://ejournal2.undip.ac.id/index.php/jplp/article/view/29947}
}

Citation Format:

Abstract

Citronella oil is one of the essential oils, where the main component is Citronellal. The Instrumental Chemistry Laboratory is a testing laboratory that accepts citronella oil testing samples from both Chemistry Study Program students at the Indonesian University of Education and external students. As a testing laboratory, the analysis must use proven methods, procedures, and equipment to ensure the suitability and purpose of the test. Currently, the Instrumental Chemistry Laboratory will propose and implement ISO/IEC 17025 so that the test results are more valid, consistent, and accountable. The purpose of this study was to verify the method for determining citronellal levels in citronella oil using GC-FID, where the samples used were samples from the isolation and synthesis of organic compounds practicum by chemistry study program students. The results of the study for the linearity of the citronellal standard obtained a correlation value of 0.9988 and has entered the linearity quality requirement of 0.9950. From the results of the accuracy test measurements, the recovery value was 91.15% and entered the quality requirement of 80-110%. From the results of the precision test measurements, the RSD value was 1.87%, which was within the quality requirements of <2%. The limit of detection and limit of quantitation were determined with LOD results of 2.72% and LOQ of 9.06%. Based on the results of the verification study, the method for analyzing citronellal levels in citronella oil using GC-FID has met the acceptance requirements, so it can be concluded that this method has been verified

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Keywords: method validation, citronella, citronellal, GC-FID

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Language : EN

In Vol. 8 No. 2, Juli 2026 (Issue In Progress)

Aryasa IWT, Artini NPR, Risky DP, Hendrayana IMD. Kadar Alkohol Pada Minuman Tuak Desa Sanda Kecamatan Pupuan Menggunakan Metode Kromatografi Gas K. J Ilm Medicam. 2019;5(1):33–8. doi: https://doiorg/10.36733/medicamento.v5i1.837
Al-Rubaye AF, Hameed IH, Kadhim MJ. A Review: Uses of Gas Chromatography-Mass Spectrometry (GC-MS) Technique for Analysis of Bioactive Natural Compounds of Some Plants. Int J Toxicol Pharmacol Res. 2017 Mar;9(01). doi: http://dx.doi.org/10.25258/ijtpr.v9i01.9042
Harianingsih, Wulandari, R., Harliyanto, C., & Andiani, C. N., “Identifikasi GC-MS Ekstrak Minyak Atsiri dari Sereh Wangi (Cymbopogon winterianus) Menggunakan Pelarut Metanol”, Techno, ISSN 1410 – 8607, Volume 18 No. 1, 023 – 027, 2017
Ketaren, S. Pengantar Teknologi Minyak Atsiri. Jakarta : Balai Pustaka;1985
Khopkar, S.M.(1990). Konsep Dasar Kimia Analitik.Jakarata: Universitas Indonesia
Milone, C., dkk, 2000, Selesctive One Step Synthesis Of (-)- Mentol From (+)- Citronellal on ru Support on Modified SiO2, Applied Catalyst A: General, 199,2000, 239-244
Mutaqin, A., Sayekti, E., Destiarti, L., 2013, Identifikai Hasil Reaksi Adisi Nukleofilik Sianida pada Gugus Karbonil Sitronelal Menggunakan Pereaksi Kalium Sianida, JKK, 2(1):38-41
Putri, A.S & Windy Z. (2021). Studi Literatur Isolasi Minyak Nilam (Pogostemon Cablin Benth) dari Beberapa Metode Distilasi. Jurnal Teknologi Separasi Distilat. 7(2) : 552-560
Pybus, D., Sell, C., Eds, 1999, The Chemistry of Fragrance, Di Dalam Lenardoa, E.J., dkk, 2007, Citronellal as Key Compound in Organic Synthesis, Tetrahedron, 63, 2007, 6671-6712.3
Rizalina, H., Cahyono, E., Mursiti, S., Nurcahyo, B., & Supartono, “Optimasi Penentuan Kadar Metanol dalam Darah Menggunakan Gas Chromatography”, Indonesian Journal of Chemical Science, 7 (3), 2018
Ruwindya,”Validasi Metode Penentuan Sitronelal dalam Minyak Atsiri Sereh Wangi secara Kromatografi GasIndonesian Journal of Chemical Analysis Vol. 05, No 02, 2022, pp. 95-102)
Sastrohamidjojo, H., “Kimia Minyak Atsiri”, Yogyakarta: FMIPA, Universitas Gadjah Mada 2002
Sulaswatyy dan Adilina, I. B., “Quo Vadis Minyak Serai Wangi dan Produk Turunannya”, Jakarta: LIPI Press, 2019
Sumar, hendayana (1994). Kimia Analisis Instrumen . Jakarta: PT.Gramedia Pustaka Umum
Yulvianti, M., Sari, R. M., & Amaliah, E. F., “Pengaruh Perbandinagn Campuran Pelarut N-Heksana- Etanol Terhadap Kandungan Sitronelal Hasil Ekstraksi Sereh Wangi (Cymbopogon nardus)”, Jurnal Integrasi Proses, Vol. 5, No. 1, 8 – 14, 2014
Yuneka, (2000). Teknik Kromatografi. Jakrta:. PT. kalman Pustaka

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