skip to main content

Validasi Metode Pengukuran Kadar Total Flavonoid dalam Ekstrak Etanol Sirih Merah (Piper crocatum var. Ruiz & Pav) Menggunakan Nano Spektrofotometer BMG Lab. Tech.

Departemen Biokimia Fakultas Matematika dan Ilmu Pengetahuan Alam Institut Pertanian Bogor, Bogor, Indonesia

Open Access Copyright 2025 Jurnal Pengelolaan Laboratorium Pendidikan

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Citation Format:
Abstract

Method validation is a crucial step when an analytical method is applied using different instruments to ensure the accuracy, precision, and reliability of the results. This study aimed to validate the method for determining the total flavonoid content in the 70% ethanol extract of red betel leaves (Piper crocatum) using a BMG LAB.TECH nanospectrophotometer equipped with a 96-well plate. This method offers advantages in terms of time efficiency and sample volume compared to conventional spectrophotometric techniques. The validation parameters assessed included linearity, precision, accuracy, limit of detection (LoD), and limit of quantitation (LoQ). The results demonstrated excellent linearity with a correlation coefficient (r) of 0.997. Precision testing yielded a relative standard deviation (%RSD) of 2.65%, while accuracy testing showed an average recovery rate of 104.33%. The LoD and LoQ were calculated to be 4.06 ppm and 13.52 ppm, respectively. Based on these findings, the total flavonoid content in the ethanol extract of the red betel leaves was determined to be 24.30 mg QE/g. Because the method meets the validation criteria outlined in the ICH guidelines, it is considered valid and suitable for use in educational laboratory activities and scientific research applications.

Note: This article has supplementary file(s).

Fulltext View|Download |  Copyright Transfer Agreement
Validasi Metode Pengukuran Kadar Total Flavonoid dalam Ekstrak Etanol Sirih Merah (Piper crocatum var. Ruiz & Pav) Menggunakan Nano Spektrofotometer BMG Lab. Tech.
Subject Flavonoid, Nanospektrofotometer, Sirih merah, Spektrofotometri UV-Vis, Validase metode
Type Copyright Transfer Agreement
  Download (1MB)    Indexing metadata
Keywords: Flavonoid, Extraction, Method validation, Nanospectrophotometer, Red betal leaf

Article Metrics:

  1. Agati, G., Brunetti, C., Fini, A., Gori, A., Guidi, L., Landi, M., Sebastiani, F., & Tattini, M. (2020). Are flavonoids effective antioxidants in plants? Twenty years of our investigation. Antioxidants, 9(11), 1–17. https://doi.org/10.3390/antiox9111098
  2. Anisah, S. U., Darmawati, A., & Prawita, A. (2021). Validation of UV Spectrophotometry Method for Determination of Lopinavir and Ritonavir Simultaneously. Berkala Ilmiah Kimia Farmasi, 8(2), 48–54. https://doi.org/10.20473/bikfar.v8i2.31760
  3. AOAC International. (2016). Official methods of analysis of AOAC International (20th ed.). Gaithersburg, MD: AOAC International
  4. Badan Standardisasi Nasional. (1992). SNI 01-2891-1992: Cara uji makanan dan minuman. Jakarta: BSN
  5. Chairunisa, F., Safithri, M., & Bintang, M. (2022). Antibacterial Activity of Ethanol Extract of Red Betel Leaves (Piper crocatum) and Its Fractions against Escherichia coli pBR322 (Aktivitas Antibakteri Ekstrak Etanol Daun Sirih Merah (Piper crocatum) dan Fraksinya terhadap Escherichia coli pBR322. Curr. Biochem, 9(1), 1–15
  6. Chandra, M. A. (2023). Verifikasi Metode Analisis Larutan Quercetin Menggunakan Spektrofotometer UV-Vis (T60). Borneo Journal of Pharmascientech, 07(02), 59–64. https://doi.org/10.51817/bjp.v7i1.484
  7. Cong-Hau, N., Anh-Dao, L.-T., Nhon-Duc, L., & Thanh-Nho, N. (2021). Spectrophotometric Determination of Total Flavonoid Contents in Tea Products and Their Liquors Under Various Brewing Conditions. Malaysian Journal of Analytical Sciences, 25, 740–750
  8. Fajri, N., Prima, E. C., Riandi, R., & Sriyati, S. (2024). Validasi Metode Analisis Konsentrasi Larutan Kopi berdasarkan Spektroskopi Absorpsi Cahaya. JIPFRI (Jurnal Inovasi Pendidikan Fisika Dan Riset Ilmiah), 8(1), 51–59. https://doi.org/10.30599/jipfri.v8i1.2101
  9. Handoyo, T. R., Purnomo, G. A., Maryanto, C. D., & Gani, M. R. (2022). Validasi dan Penetapan Kadar Senyawa Rutin pada Ekstrak Etanol Daun Binahong (Anredera cordifolia (Ten.) Steenis) dengan Metode KCKT. Fitofarmaka: Jurnal Ilmiah Farmasi, 12(1), 1–13. https://doi.org/10.33751/jf.v12i1.3596
  10. Kurniasari, R., Suzery, M., & Cahyono, B. (2024). Analysis of Total Phenolics, Flavonoids, and Antioxidant Activity of Cashew Leaf Extract (Anacardium occidentale L.) with Varying Ethanol Concentrations. Jurnal Riset Kimia, 15(2), 116–130. https://doi.org/10.25077/jrk.v15i2.735
  11. Nugroho, S. A., & Alrayan, R. (2024). Validasi Metode Analisa Piroksikam pada Sediaan Self Nano Emulsifying Drug Delivery System (SNEDDS) menggunakan Spektrofotometri UV-Vis. Jurnal Pharma Bhakta, 4(1), 1–7
  12. Nurhayati, S., & Yuliana, R. (2022). Penentuan Batas Deteksi dan Batas Kuantifikasi Metode Spektrofotometri UV-Vis untuk Senyawa Fenolik dalam Ekstrak Daun. Pharmacy: Jurnal Farmasi Indonesia, 19(1), 78–85
  13. Nurwanti, R., Hamzah, H., Yolandari, S., Ode, W., Ds, A., & Baubau, P. (2024). Penetapan Kadar Flavonoid Total Ekstrak Etanol Daun Binahong (Anredera cordifolia) Dengan Metode Spektrofotometri UV-Vis. Jurnal Promotif Preventif, 7(3), 642–650. http://journal.unpacti.ac.id/index.php/JPP
  14. Ramos, R., Bezerra, I., Ferreira, M., & Soares, L. (2017). Spectrophotometric quantification of flavonoids in herbal material, crude extract, and fractions from leaves of Eugenia uniflora Linn. Pharmacognosy Research, 9(3), 253–260. https://doi.org/10.4103/pr.pr_143_16
  15. Rustamsyah, A., Kartini, H., Martiani, I., & Sujana, D. (2023). Analisis Fenol dan Flavonoid Total Pada Beberapa Teh Putih (Camellia sinensis L.) yang Beredar di Pasaran. Teknotan, 16(3), 177–181. https://doi.org/10.24198/jt.vol16n3.7
  16. Putri, D. A., Nugroho, L. H., & Lestari, D. A. (2020). Validasi metode spektrofotometri untuk penetapan kadar flavonoid total pada ekstrak etanol tanaman herbal. Jurnal Ilmu Kefarmasian Indonesia, 18(2), 89–95. https://doi.org/10.35814/jifi.v18i2.1362
  17. Putri, V. S., Ikhsan, A. N., Martien, R., & Adhyatmika. (2023). Validation of Uv-Vis Spectrophotometric Method to Determine Drug Release of Quercetin Loaded-Nanoemulsion. Indonesian Journal of Pharmacy Indonesian J Pharm, 34(2), 272–279
  18. Wahyuningtyas, F., Arifin, I., & Anwar, K. (2025). Aktivitas Antioksidan Ekstrak Etanol Kulit Buah Sukun (Artocarpus altilis (Park.) Fosberg) Serta Penentuan Kadar Fenolik dan Flavonoid Total. Jurnal Ilmiah Sains, 25(1), 1–12. https://doi.org/10.35799/jis.v25i1
  19. Yasa, I., Putra, N., & Wiadnyani, A. (2019). Pengaruh Konsentrasi Etanol Terhadap Aktivitas Antioksidan Ekstrak Daun Sirih Merah (Piper crocatum Ruitz & Pav) Menggunakan Metode Microwave Assisted Extraction (MAE). Jurnal Ilmu Dan Teknologi Pangan, 8(3), 278–284
  20. Yunita, E., Yulianto, D., Fatimah, S., & Firanita, T. (2020). Validation of UV-Vis Spectrophotometric Method of Quercetin in Ethanol Extract of Tamarind Leaf. Journal of Fundamental and Applied Pharmaceutical Science, 1(1), 11–18. https://doi.org/10.18196/jfaps.010102

Last update:

No citation recorded.

Last update:

No citation recorded.