DOI: https://doi.org/10.14710/jplp.6.2.126-132

Efektifitas Perasan Kulit Mangis Sebagai Pengganti Eosin 2% Pada Pemeriksaan Telur Cacing

Laboratorium Keperawatan, Fakultas Keperawatan , Indonesia

Open Access Copyright 2024 Jurnal Pengelolaan Laboratorium Pendidikan

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Citation Format:
Abstract

Examination of Intestinal Nematode worm eggs is one of the practicum subjects in the Basic Science of Nursing course at the Biomedical Laboratory of the Faculty of Nursing, University of Riau. Eosin 2% is a stain used in the examination of Intestinal Nematode worm eggs. However, the availability of 2% eosin cannot be sufficient to facilitate every student. This is because the procurement of consumables in the laboratory is very limited. An alternative new coloring method is needed using natural ingredients, one of which is derived from mangosteen peel juice. This study aims to determine the effectiveness of the percentage of mangosteen peel juice that can be used as a substitute for 2% eosin. The research design was an experimental study using a completely randomized design (CRD). The subjects of the study were direct staining of mangosteen peel juice and 2% eosin as a control. The object of research is worm positive feces. As a treatment is mangosteen peel which consists of 2 (two) times the level of treatment, namely mangosteen peel juice 5%, 10%. The results showed that a concentration of 10% mangosteen peel juice was effective in examining worm eggs as a substitute for eosin. Based on the results obtained, it can be concluded that mangosteen peel juice is effective as a substitute for 2% eosin in staining worm eggs, namely at a concentration of 10%.

Fulltext View|Download

Article Metrics:

Article Info
Section: Articles
Language : ID
Recent articles
Efektifitas Perasan Kulit Mangis Sebagai Pengganti Eosin 2% Pada Pemeriksaan Telur Cacing Rancang Bangun Sheet Metal Rotary Cutting Shears Guna Mendukung Kinerja Laboratorium Kerja Plat Suplementasi Ekstrak Daun Ketapang (Terminalia catappa) pada Potato Sucrose Agar (PSA) untuk Menggantikan Dichloran Rose Bengal Chloramphenicol (DRBC) pada Praktikum Mikrobiologi Pangan dan Pengolahan More recent articles
  1. Apriani dan Ereskadi. 2022. Ektrak Kulit Manggis (Garcinia Mangostana L) Sebagai Alternatif Pengganti Eosin Untuk Pemeriksaan Telur Cacing. Journal of Indonesian Medical Laboratory and Science 3 (1) : 80 – 88
  2. Kartini, S., dan Angelia, E. 2021. Utilization of Juice Beta vulgaris L. as an Alternative Reagent for Examination of Worm Eggs Ascaris lumbricoides. JPK : Jurnal Proteksi Kesehatan. 10(1) : 20 – 25
  3. Kurniawati, A. 2020. Ekstraksi Dan Analisis Zat Warna Ekstrak Kulit Buah Manggis (Garcinia mangostana L.) Serta Aplikasinya Sebagai Indikator Asam Basa. Tesis, Universitas Negeri Semarang, Semarang
  4. Mariana, S., Permana, A.W, dan Kusumaningrum, H.D. 2009. Kapasitas dan Kadar Antioksidan Ekstrak Tepung Kulit Buah Manggis (Garcinia Mangostana L.) Pada Berbagai Pelarut Dengan Metode Maserasi. Jurnal Penelitian Pasca Panen 6 (2) : 61 – 68
  5. Munawaroh, S., dan Shofi, M. 2023. Modifikasi Pewarna Alami Ekstrak Etanol Sappan Lignum pada Pewarnaan Soil Transmitted Helminth Pengganti Eosin 2%. Bioscientist: Jurnal Ilmiah Biologi 11 (2) : 1553 – 1562
  6. Oktari, A dan Mu’tamir, A. 2017. Optimasi Air Perasan Buah Merah (pandanus sp.) Pada Pemeriksaan Telur Cacing. Jurnal Teknologi Laboratorium 6 (1) : 8 – 17
  7. Ozawa, A., & Sakaue, M. 2020. New Decolorization Method Produces More Information from Tissue Sections Stained with Hematoxylin and Eosin Stain and Masson-Trichrome Stain. Annals of Anatomy-Anatomischer Anzeiger. 227(151431) : 1-24
  8. Pustiari, P. A., Leliqia, N. P. E., dan Wijayanti, N. P. A. D. 2014. Penentuan Rendemen Antosianin Total Ekstrak Kulit Buah Manggis (Garcinia Mangostana L.) dengan Pengeringan Oven. Jurnal Farmasi Udayana 3 (2) : 9 – 12
  9. Rahma, N.A., Zanaria, T.M., Nurjannah, Husna, F., Putra, T.R.I. 2020. Faktor Resiko Terjadinya Kecacingan pada Anak Usia Sekolah Dasar. Jurnal Kesehatan Masyarakat Indonesia, 15 (2) : 29 – 33
  10. Samber, L.N., Semangun H, dan Prasetyo B. 2013. Karakteristik Antosianin Sebagai Pewarna Alami. Proceeding Biology Education Conference 10 (3) : 1 – 4
  11. Triana, L. 2021. Utilization of Andong Leaf Extract (Cordyline fruticosa) as an Alternative for Coloring the Eggs of Intestinal Nematode. Jurnal Teknologi Kesehatan Borneo 2(2) : 108-112
  12. WHO. 2017. Soil-transmitted helminth infections. https://www.who.int/news-room/fact-sheets/detail/soil-transmitted-helminth-infections, diakses pada 18 Januari 2023

Last update:

No citation recorded.

Last update:

No citation recorded.