Separation of Saponin Using Nanofiltration Membrane

*Nur 'Aini Hamada  -  Membrane Research Center, Integrated Laboratory for Research and Services, Universitas Diponegoro, Indonesia
Ilham Dwiyanto Emzar  -  1. Department of Chemical Engineering, Faculty of Engineering, Diponegoro University, Indonesia
Yuniar Luthfia Listyadevi  -  1. Department of Chemical Engineering, Faculty of Engineering, Diponegoro University, Indonesia
Open Access Copyright 2021 Journal of Membranes and Materials under http://creativecommons.org/licenses/by-sa/4.0.

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Abstract
Separation of pure saponin and saponin-BSA protein mixture by nanofiltration membranes have been investigated in this study to understand the nanofiltration potential to obtain high purity saponin. Commercial NF membranes: NF, NF270, and DSS-ETNA01PP were used. The effects of the operating conditions such as pressure, the concentration of feed, and the composition of feed were evaluated. The permeate flux and rejection rate of saponin and saponin-BSA were the criteria of this evaluation. The increasing operating pressure increased the permeate flux. In addition to the membranes‘ MWCO, electrostatic repulsion between the charged membrane interface and solute determined the saponin and saponin-BSA solution's rejection rate. The flux of pure saponin feed was greater but generated lower rejection rates than the saponin-BSA feed.  Increasing feed concentration resulted in an increased rejection rate. However, the flux decreased with increasing pure saponin concentration but increased with a higher dose of saponin-BSA. The DSS-ETNA01PP membrane had the largest flux value and the smallest rejection value compared to other membranes. The results indicated that nanofiltration was potential for the saponin purifying process.
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Keywords: Electrostatic; Nanofiltration; Rejection; Repulsion
Article Info
Section: Articles
Language : EN
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  2. Bart, H.J. Extraction of natural products from plants—An introduction. Industrial scale natural products extraction, 1st ed.; Wiley-VCH: Verlag, 2011; pp. 1–26
  3. Carvalho, A.I.; Maugeri, F.; Pradanos, P.; Silva V.; Hernandez A, 2011. Separation of potassium clavulanate and potassium chloride by nanofiltration transport and evaluation of membranes, Separation and Purification Technology, 83, 23-30
  4. Chaiyasut, C.; Tsuda T, 2001. Isoelectric Points Estimation of Proteins by Electroosmotiv Flow: pH Relationship Using Physically Adsorbed Proteins on Silica Gel, Chromatography, 22(2)
  5. Giichi, H. 1987. Production of saponin containing no isoflavone from soybean embryo bud. JP Patent 62,005,917
  6. Guclu-Untundag, O.; Mazza, G, 2007. Saponins: Properties, Applications and Processing. Critical Reviews in Food Science and Nutrition, 47, 231–258
  7. Hostettmann, K.; Marston, A. Saponins (Chemistry and Pharmacology of Natural Products). Cambridge University Press: Cambridge, 1995
  8. Kensil, C. A.; Marciani, D. J. 1991. Saponin adjuvant. US Patent 5,057,540
  9. Kezwon, A.; Wojciechowski, K., 2014. Interaction of Quillaja bark saponins with food-relevant proteins. Advances in Colloid and Interface Science, 209, 185-195
  10. Kitagawa, I. 1986. Method of isolating soya saponins. US Patent 4,594,412
  11. Lin, C. J.; Rao P.; Shirazi, S, 2004. Effect of operating parameters on permeate flux decline caused by cake formation – a model study, Desalination, 171, 95-105
  12. Norman, L.N.; Fane, A G.; Winston Ho, W.S.; Matsuura T. Nanofiltration. In Advanced Membrane Technology and Applications; John Willey & Sons, Inc., New Jersey, USA, 2008, pp. 271
  13. Nozomi, O.; Haruo, S.; Shisai, R.; Fuku, S.; Hikari, J.; Toshi, H.; Bunshi, K. 1986. Saiko saponin. JP Patent 61,282,395
  14. Pedebos, C.; Pol-Fachin, L.; Pons, R.; Teixeira, C.V.; Verli, H., 2014. Atomic Model and Micelle Dynamics of QS-21 Saponin. Molecules, 19, 3744-3760
  15. Sparg, S. G.; Light, M. E.; van Staden, J., 2004. Biological activities and distribution of plant saponins. Journal of Ethnopharmacology, 94, 219–243
  16. Salgin, S.; Salgin, U.; Bahadir, S., 2012. Zeta Potensials and Isoelectric Points of Biomolecules: The Effect of Ion Types and Ionic Strenghts
  17. International Journal of Electrochemical Science, 7, 12404 – 12414
  18. Susanto, H.; Stahra, N.; Ulbricht, M., 2009. High performance polyethersulfone microfiltration membrane having high flux and stable hydrophilic property. Journal of Membrane Science, 342, 153-164
  19. Sutzkover-Gutman, I.; Hasson, D.; Semiat, R.; 2010. Humic substances fouling in ultrafiltration processes. Desalination, 261(3), 218-231
  20. Takeuchi, T. M.; Pereira, C. G.; Braga, M. E. M.; Maróstica, M. R.; Leal, P. F.; Meireles, M. A.A. 2009. Low-pressure solvent extraction (solid–liquid extraction, microwave assisted, and ultrasound assisted) from condimentary plants. In M. A. A. Meireles (Ed.), Extracting bioactive compounds for food products—Theory and applications (pp. 140–144). Boca Raton: CRC Press, 151-158
  21. Vongsangnak, W.; Gua, J.; Chauvatcharin, S.; Zhong, J. -J., 2004. Towards efficient extraction of notoginseng saponins from cultured cells of Panax notoginseng. Biochemical Engineering Journal, 18, 115–120
  22. Wei, J.; Helm, G.S.; Corner-Walker, N.; Hou, X., 2006. Characterization of a non-fouling ultrafiltration membrane. Desalination, 192, 252-261
  23. Wu, J.; Lin, L.; Chau, L., 2001. Ultrasound-assisted Extraction of Ginseng Saponins from Ginseng Roots and Cultured Ginseng Cells. Ultrasonics Sonochemistry, 8, 347–352

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