Departemen Ilmu Kelautan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro, Indonesia
BibTex Citation Data :
@article{JKT595, author = {Wilis Setyati and Ahmad Habibi and Subagiyo Subagiyo and Ali Ridlo and Nirwani Soenardjo and Rini Pramesti}, title = {Skrining Dan Seleksi Bakteri Simbion Spons Penghasil Enzim Ekstraseluler Sebagai Agen Bioremediasi Bahan Organik Dan Biokontrol Vibriosis Pada Budidaya Udang}, journal = {Jurnal Kelautan Tropis}, volume = {19}, number = {1}, year = {2016}, keywords = {Bakteri simbion spons; enzim ekstraseluler; bioremediasi; vibriosis; Sponge symbiotic bacteria; extracellular enzymes; bioremediation}, abstract = { The rapid cultivation of aquaculture with the application of intensive systems in recent years, has raised problems in the form of a decrease in the carrying capacity of ponds for the life of aquaculture organisms. The further impact caused is the occurrence of a series of diseases and damage to environmental conditions. Anticipatory measures through the application of bioremediation are solutions to prevent more serious damage. In the bioremediation process, enzymes play the role of catalysts that accelerate biochemical reactions in ponds of soil and water. Bacteria associated with sponges have various bioactive compounds that can inhibit the growth of pathogenic bacteria. The purpose of this study is isolation and screening of bacteria associated with sponges that have extracellular enzyme activity (proteolytic, amylolytic, cellulolytic, and lipolytic) and antibacterial activity, as well as knowing the interaction of antagonism properties among the best isolates, and phenotypic identification of bacterial species. This research was conducted in 4 stages: sample collection, isolation, selection, and identification. The results showed that the total sponge symbiotic bacteria obtained were 15 isolates with the potential of 15 proteolytic isolates, 12 amylolytic isolates, 12 lipolytic isolates, and 4 cellulolytic isolates. There were 10 isolates having antibacterial activity against Vibrio harveyi and 2 isolates had antibacterial activity against Vibrio alginolyticus. The best bacterial isolates SP.1.3, SP.5.1, and SP.5.3 have no antagonistic activity between the three. Phenotypic identification of 3 isolates alleged that isolates SP.1.3, SP.5.1, and SP.5.2 were identified as Bacillus sp., Acinetobacter sp., And Pseudomonas sp. Pesatnya kegiatan budidaya perikanan dengan penerapan sistem intensif dalam beberapa tahun terakhir, telah memunculkan permasalahan berupa penurunan daya dukung tambak bagi kehidupan organisme budidaya. Dampak lanjut yang ditimbulkan adalah terjadinya serangkaian penyakit dan kerusakan kondisi lingkungan. Langkah antisipatif melalui penerapan bioremediasi merupakan solusi untuk mencegah kerusakan yang lebih serius. Dalam proses bioremediasi, enzim memainkan peran katalis yang mempercepat reaksi biokimia di kolam tanah dan air. Bakteri yang berasosiasi dengan spons memiliki beragam senyawa bioaktif yang dapat menghambat pertumbuhan bakteri patogen. Tujuan dari penelitian ini adalah isolasi dan skrining bakteri yang berasosiasi dengan spons yang memiliki aktivitas enzim ekstraseluler (proteolitik, amilolitik, selulolitik, dan lipolitik) dan aktivitas antibakteri, serta mengetahui interaksi sifat antagonisme antar isolat terbaik, dan identifikasi spesies bakteri secara fenotipik. Penelitian ini dilakukan dalam 4 tahap: koleksi sampel, isolasi, seleksi, dan identifikasi. Hasil penelitian menunjukkan bahwa total bakteri simbion spons yang diperoleh 15 isolat dengan potensi 15 isolat proteolitik, 12 isolat amilolitik, 12 isolat lipolitik, dan 4 isolat selulolitik. Terdapat 10 isolat memiliki aktivitas antibakteri terhadap Vibrio harveyi dan 2 isolat memiliki aktivitas antibakteri terhadap Vibrio alginolyticus. Isolat bakteri terbaik SP.1.3, SP.5.1, dan SP.5.3 tidak memiliki aktivitas saling antagonis antar ketiganya. Identifikasi fenotipik dari 3 isolat diduga bahwa isolat SP.1.3, SP.5.1, dan SP.5.2 diidentifikasi sebagai Bacillus sp., Acinetobacter sp., dan Pseudomonas sp. }, issn = {2528-3111}, pages = {11--20} doi = {10.14710/jkt.v19i1.595}, url = {https://ejournal2.undip.ac.id/index.php/jkt/article/view/595} }
Refworks Citation Data :
The rapid cultivation of aquaculture with the application of intensive systems in recent years, has raised problems in the form of a decrease in the carrying capacity of ponds for the life of aquaculture organisms. The further impact caused is the occurrence of a series of diseases and damage to environmental conditions. Anticipatory measures through the application of bioremediation are solutions to prevent more serious damage. In the bioremediation process, enzymes play the role of catalysts that accelerate biochemical reactions in ponds of soil and water. Bacteria associated with sponges have various bioactive compounds that can inhibit the growth of pathogenic bacteria. The purpose of this study is isolation and screening of bacteria associated with sponges that have extracellular enzyme activity (proteolytic, amylolytic, cellulolytic, and lipolytic) and antibacterial activity, as well as knowing the interaction of antagonism properties among the best isolates, and phenotypic identification of bacterial species. This research was conducted in 4 stages: sample collection, isolation, selection, and identification. The results showed that the total sponge symbiotic bacteria obtained were 15 isolates with the potential of 15 proteolytic isolates, 12 amylolytic isolates, 12 lipolytic isolates, and 4 cellulolytic isolates. There were 10 isolates having antibacterial activity against Vibrio harveyi and 2 isolates had antibacterial activity against Vibrio alginolyticus. The best bacterial isolates SP.1.3, SP.5.1, and SP.5.3 have no antagonistic activity between the three. Phenotypic identification of 3 isolates alleged that isolates SP.1.3, SP.5.1, and SP.5.2 were identified as Bacillus sp., Acinetobacter sp., And Pseudomonas sp.
Pesatnya kegiatan budidaya perikanan dengan penerapan sistem intensif dalam beberapa tahun terakhir, telah memunculkan permasalahan berupa penurunan daya dukung tambak bagi kehidupan organisme budidaya. Dampak lanjut yang ditimbulkan adalah terjadinya serangkaian penyakit dan kerusakan kondisi lingkungan. Langkah antisipatif melalui penerapan bioremediasi merupakan solusi untuk mencegah kerusakan yang lebih serius. Dalam proses bioremediasi, enzim memainkan peran katalis yang mempercepat reaksi biokimia di kolam tanah dan air. Bakteri yang berasosiasi dengan spons memiliki beragam senyawa bioaktif yang dapat menghambat pertumbuhan bakteri patogen. Tujuan dari penelitian ini adalah isolasi dan skrining bakteri yang berasosiasi dengan spons yang memiliki aktivitas enzim ekstraseluler (proteolitik, amilolitik, selulolitik, dan lipolitik) dan aktivitas antibakteri, serta mengetahui interaksi sifat antagonisme antar isolat terbaik, dan identifikasi spesies bakteri secara fenotipik. Penelitian ini dilakukan dalam 4 tahap: koleksi sampel, isolasi, seleksi, dan identifikasi. Hasil penelitian menunjukkan bahwa total bakteri simbion spons yang diperoleh 15 isolat dengan potensi 15 isolat proteolitik, 12 isolat amilolitik, 12 isolat lipolitik, dan 4 isolat selulolitik. Terdapat 10 isolat memiliki aktivitas antibakteri terhadap Vibrio harveyi dan 2 isolat memiliki aktivitas antibakteri terhadap Vibrio alginolyticus. Isolat bakteri terbaik SP.1.3, SP.5.1, dan SP.5.3 tidak memiliki aktivitas saling antagonis antar ketiganya. Identifikasi fenotipik dari 3 isolat diduga bahwa isolat SP.1.3, SP.5.1, dan SP.5.2 diidentifikasi sebagai Bacillus sp., Acinetobacter sp., dan Pseudomonas sp.
Article Metrics:
Last update:
Upon acceptance for publication, authors agree to transfer the copyright of their article to Jurnal Kelautan Tropis, while retaining the right to reuse their work under the terms of the open license applied.
From the date of publication, the copyright for each article is held by Jurnal Kelautan Tropis. This transfer allows the journal to manage, disseminate, and preserve scholarly content in accordance with international standards and open access best practices.
Although copyright is held by the journal, all published articles are made available under the Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA 4.0). Under this license, anyone may:
Copy and redistribute the material in any medium or format
Remix, transform, and build upon the material for any purpose, even commercially
provided that:
Appropriate credit is given to the original author(s) and the source
Indications are made of any changes that were made
Derivative works are distributed under the same license (CC BY-SA 4.0)
While copyright is held by the journal, authors retain important reuse rights. Authors may:
Reuse the published version of their article in future works, including books, compilations, and lectures
Deposit the published version in institutional or subject repositories
Share the article freely, including on personal websites or academic networks
as long as the original publication in Jurnal Kelautan Tropis is cited and the CC BY-SA 4.0 license terms are respected.
Authors must ensure that any third-party content included in the article (e.g., figures, images, datasets) is either original, in the public domain, or licensed for reuse under compatible terms. If specific permissions are required, authors must obtain them prior to submission.
For questions regarding copyright or licensing, please contact the editorial office at: j.kelautantropis@gmail.com
View My Stats
Jurnal Kelautan Tropis is published by Departement of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro under a Creative Commons Attribution-ShareAlike 4.0 International License.
