Departemen Ilmu Kelautan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro, Indonesia
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@article{JKT4669, author = {Wahyu Febrianto and Ali Djunaedi and Suryono Suryono and Gunawan Widi Santosa and Sunaryo Sunaryo}, title = {Potensi Antioksidan Rumput Laut Gracilaria verrucosa Dari Pantai Gunung Kidul, Yogyakarta}, journal = {Jurnal Kelautan Tropis}, volume = {22}, number = {1}, year = {2019}, keywords = {Gracilaria verrucosa; antioksidan; DPPH; fitokimia; antioxidant; DPPH; phytochemical}, abstract = { Gracilaria verrucosa is red algae that has been widely used as an antioxidant. This research was conducted to test antioxidant activity, total phenolic content and bioactive compound of Gracilaria verrucosa obtained from Pok Tunggal Beach and Ngandong Beach, Gunung Kidul, Yogyakarta. Research was carried out by descriptive method. Samples were fresh and taken from the beach, then macerated for 3x24 hours in a methanol solvent. Antioxidant test was carried out by electron transfer method with DPPH 0.1 mM and measurement of antioxidant activity using. Total phenolic contents were measured using the Folin-ciocalteau method using gallic acid standard on 725 nm wavelength. The phytochemical content observated by changing of extract color by reagent. Pigment contents were measured using spectrophotometric methods at wavelengths 636 and 663 (chlorophyll-a and chlorophyll-b) and 480 nm (carotenoids). The results showed that IC50 value extract of Pok Tunggal Beach and Ngandong Beach were 188,53 ppm and 168,76 ppm. Phenolic content of each extract were 16,527 and 17,497 mg GAE / g sample weight). Chlorophyll-a levels were 7,132 and 4,357 mg/g, chlorophyll-b were 8,335 and 5,401 mg/g, carotenoids were 31,625 and 35,494 µmol/g. Gracilaria verrucosa from Ngandong Beach have antioxidant activity. Gracilaria verrucosa merupakan alga merah yang pemanfaatannya sudah banyak dilakukan sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui potensi antioksidan, menghitung kadar fenolat total, dan senyawa bioaktif yang terkandung pada Gracilaria verrucosa yang diperoleh dari Pantai Pok Tunggal dan Pantai Ngandong, Gunung Kidul, Yogyakarta. Penelitian ini dilakukan dengan metode deskriptif. Sampel segar diambil langsung dari pantai, kemudian dimaserasi selama 3x24 jam dalam pelarut metanol. Uji antioksidan dilakukan dengan metode transfer elektron dengan DPPH 0,1 mM dan pengukuran aktivitas antioksidan menggunakan perhitungan nilai IC 50 . Kadar fenolat total diukur menggunakan metode Folin-ciocalteau dengan asam galat sebagai standar pada panjang gelombang 725 nm. Kandungan fitokimia diuji menggunakan pengamatan perubahan warna ekstrak saat diberikan pereaksi. Kadar pigmen diukur menggunakan metode spektrofotometri pada panjang gelombang 636, 663 (klorofil-a dan klorofil-b) dan 480 nm (karotenoid). Hasil menunjukkan bahwa nilai IC 50 ekstrak sampel dari Pantai Pok Tunggal dan Pantai Ngandong berturut-turut adalah 188,53 ppm dan 168,76 ppm. Kadar fenolat masing-masing ekstrak sebesar 16,527 dan 17,497 mg GAE/g berat sampel). Kadar klorofil-a sebesar 7,132 dan 4,357 mg/g, klorofil-b sebesar 8,335 dan 5,401 mg/g, karotenoid sebesar 31,625 dam 35,494 µmol/g. Gracilaria verrucosa dari Pantai Ngandong dan Pantai Pok Tunggal memiliki potensi antioksidan. }, issn = {2528-3111}, pages = {81--86} doi = {10.14710/jkt.v22i1.4669}, url = {https://ejournal2.undip.ac.id/index.php/jkt/article/view/4669} }
Refworks Citation Data :
Gracilaria verrucosa is red algae that has been widely used as an antioxidant. This research was conducted to test antioxidant activity, total phenolic content and bioactive compound of Gracilaria verrucosa obtained from Pok Tunggal Beach and Ngandong Beach, Gunung Kidul, Yogyakarta. Research was carried out by descriptive method. Samples were fresh and taken from the beach, then macerated for 3x24 hours in a methanol solvent. Antioxidant test was carried out by electron transfer method with DPPH 0.1 mM and measurement of antioxidant activity using. Total phenolic contents were measured using the Folin-ciocalteau method using gallic acid standard on 725 nm wavelength. The phytochemical content observated by changing of extract color by reagent. Pigment contents were measured using spectrophotometric methods at wavelengths 636 and 663 (chlorophyll-a and chlorophyll-b) and 480 nm (carotenoids). The results showed that IC50 value extract of Pok Tunggal Beach and Ngandong Beach were 188,53 ppm and 168,76 ppm. Phenolic content of each extract were 16,527 and 17,497 mg GAE / g sample weight). Chlorophyll-a levels were 7,132 and 4,357 mg/g, chlorophyll-b were 8,335 and 5,401 mg/g, carotenoids were 31,625 and 35,494 µmol/g. Gracilaria verrucosa from Ngandong Beach have antioxidant activity.
Gracilaria verrucosa merupakan alga merah yang pemanfaatannya sudah banyak dilakukan sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui potensi antioksidan, menghitung kadar fenolat total, dan senyawa bioaktif yang terkandung pada Gracilaria verrucosa yang diperoleh dari Pantai Pok Tunggal dan Pantai Ngandong, Gunung Kidul, Yogyakarta. Penelitian ini dilakukan dengan metode deskriptif. Sampel segar diambil langsung dari pantai, kemudian dimaserasi selama 3x24 jam dalam pelarut metanol. Uji antioksidan dilakukan dengan metode transfer elektron dengan DPPH 0,1 mM dan pengukuran aktivitas antioksidan menggunakan perhitungan nilai IC50. Kadar fenolat total diukur menggunakan metode Folin-ciocalteau dengan asam galat sebagai standar pada panjang gelombang 725 nm. Kandungan fitokimia diuji menggunakan pengamatan perubahan warna ekstrak saat diberikan pereaksi. Kadar pigmen diukur menggunakan metode spektrofotometri pada panjang gelombang 636, 663 (klorofil-a dan klorofil-b) dan 480 nm (karotenoid). Hasil menunjukkan bahwa nilai IC50 ekstrak sampel dari Pantai Pok Tunggal dan Pantai Ngandong berturut-turut adalah 188,53 ppm dan 168,76 ppm. Kadar fenolat masing-masing ekstrak sebesar 16,527 dan 17,497 mg GAE/g berat sampel). Kadar klorofil-a sebesar 7,132 dan 4,357 mg/g, klorofil-b sebesar 8,335 dan 5,401 mg/g, karotenoid sebesar 31,625 dam 35,494 µmol/g. Gracilaria verrucosa dari Pantai Ngandong dan Pantai Pok Tunggal memiliki potensi antioksidan.
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