Departemen Ilmu Kelautan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro, Indonesia
BibTex Citation Data :
@article{JKT1742, author = {Ria Azizah and Ita Riniatsih and Delianis Pringgenis and Chrisna Suryono and Suryono Suryono}, title = {Isolasi dan Identifikasi Bakteri Pembentuk Biofilm dari Tambak Udang Balai Besar Pengembangan Budidaya Air Payau Jepara untuk Menghilangkan Amoniak}, journal = {Jurnal Kelautan Tropis}, volume = {20}, number = {2}, year = {2017}, keywords = {biofilm-forming primary bacteria; ammoniacal nitrogen; degradation; bakteri primer pembentuk biofilm; amonia nitrogen; degradasi}, abstract = { Brackish water shrimp aquaculture activities often result in organic waste from excess of unconsumed foodstuff and biological waste from shrimp biological waste. The high organic contents increase the levels of ammonia, which is toxic to shrimp and many other aqua lives. One of the most widely used organic material biodegradation system as biofilters, biofilm has not yet seen many uses in shrimp aquafarm waste management. This study aims to isolate and screen biofilm-forming primary bacteria with abilities to degrade ammoniacal nitrogen compounds. The processes involved in this study are location survey, wooden and fiber panel installation, planting of panel in the ponds, isolation of bacteria by dispersion method, purification of primary bacteria by scratch method. Ammoniacal nitrogen degradation test was performed by Microwell Plate Chromatogram Assay and UV-Vis Spectrophotometry. The analysis of the bacteria isolates found 66 primary bacteria with biofilm formation abilities. Based on qualitative analysis, 20 isolates displayed potential in degrading ammoniacal nitrogen compound and 7 isolates showed low (<10%) capacity in degrading ammoniacal nitrogen. Kegiatan budidaya udang di tambak akan menghasilkan limbah organik yang berasal dari sisa pakan yang tidak termakan maupun kotoran udang. Kandungan bahan organik yang tinggi akan meningkatkan kandungan amonia yang bersifat toksik bagi udang dan biota air lainnya. Salah satu sistem biodegradasi bahan organik yang telah banyak digunakan sebagai biofilter namun belum dimanfaatkan dalam pengolahan limbah organik tambak udang adalah biofilm. Tujuan dari penelitian ini adalah mengisolasi dan skrining bakteri primer pembentuk biofilm yang mampu mendegradasi senyawa amonia nitrogen. Untuk mencapai tujuan tersebut, maka beberapa tahap penelitian yang telah dilakukan adalah survei lokasi tambak udang, pemasangan panel bahan kayu dan fiber, penanaman panel dalam badan air tambak, mengisolasi bakteri dengan metode sebaran, purifikasi bakteri primer pembentuk biofilm dengan metode goresan. Uji oksidasi amonium nitrogen dilakukan secara kualitatif dan kuantitatif dengan metode Micro well plate chromatogram assay dan UV-Vis Spektrofotometer. Hasil penelitian menunjukkan bahwa hasil isolasi diperoleh sebanyak 66 isolat bakteri primer pembentuk biofilm. Berdasarkan uji kualitatif diperoleh 20 isolat yang memiliki potensi mendegradasi senyawa amoniurn nitrogen. Namun hasil uji kuantitatif bakteri seleksi pendegradasi amonium nitrogen menunjukkan 7 isolat yang memiliki kemampuan rendah (< 10%) mendegradasi amonium nitrogen. }, issn = {2528-3111}, pages = {154--160} doi = {10.14710/jkt.v20i2.1742}, url = {https://ejournal2.undip.ac.id/index.php/jkt/article/view/1742} }
Refworks Citation Data :
Brackish water shrimp aquaculture activities often result in organic waste from excess of unconsumed foodstuff and biological waste from shrimp biological waste. The high organic contents increase the levels of ammonia, which is toxic to shrimp and many other aqua lives. One of the most widely used organic material biodegradation system as biofilters, biofilm has not yet seen many uses in shrimp aquafarm waste management. This study aims to isolate and screen biofilm-forming primary bacteria with abilities to degrade ammoniacal nitrogen compounds. The processes involved in this study are location survey, wooden and fiber panel installation, planting of panel in the ponds, isolation of bacteria by dispersion method, purification of primary bacteria by scratch method. Ammoniacal nitrogen degradation test was performed by Microwell Plate Chromatogram Assay and UV-Vis Spectrophotometry. The analysis of the bacteria isolates found 66 primary bacteria with biofilm formation abilities. Based on qualitative analysis, 20 isolates displayed potential in degrading ammoniacal nitrogen compound and 7 isolates showed low (<10%) capacity in degrading ammoniacal nitrogen.
Kegiatan budidaya udang di tambak akan menghasilkan limbah organik yang berasal dari sisa pakan yang tidak termakan maupun kotoran udang. Kandungan bahan organik yang tinggi akan meningkatkan kandungan amonia yang bersifat toksik bagi udang dan biota air lainnya. Salah satu sistem biodegradasi bahan organik yang telah banyak digunakan sebagai biofilter namun belum dimanfaatkan dalam pengolahan limbah organik tambak udang adalah biofilm. Tujuan dari penelitian ini adalah mengisolasi dan skrining bakteri primer pembentuk biofilm yang mampu mendegradasi senyawa amonia nitrogen. Untuk mencapai tujuan tersebut, maka beberapa tahap penelitian yang telah dilakukan adalah survei lokasi tambak udang, pemasangan panel bahan kayu dan fiber, penanaman panel dalam badan air tambak, mengisolasi bakteri dengan metode sebaran, purifikasi bakteri primer pembentuk biofilm dengan metode goresan. Uji oksidasi amonium nitrogen dilakukan secara kualitatif dan kuantitatif dengan metode Micro well plate chromatogram assay dan UV-Vis Spektrofotometer. Hasil penelitian menunjukkan bahwa hasil isolasi diperoleh sebanyak 66 isolat bakteri primer pembentuk biofilm. Berdasarkan uji kualitatif diperoleh 20 isolat yang memiliki potensi mendegradasi senyawa amoniurn nitrogen. Namun hasil uji kuantitatif bakteri seleksi pendegradasi amonium nitrogen menunjukkan 7 isolat yang memiliki kemampuan rendah (< 10%) mendegradasi amonium nitrogen.
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