Aktivitas Antibakteri Isolat Bakteri Asam Laktat Intestinal Udang Penaeid Tipe Liar Terhadap Bakteri Vibrio

*Subagiyo Subagiyo  -  Departemen Ilmu Kelautan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro, Indonesia
Triyanto Triyanto  -  Fakultas Pertanian, Universitas Gadjah Mada, Indonesia
Sebastian Margino  -  Fakultas Pertanian, Universitas Gadjah Mada, Indonesia
Feri Setiawan  -  Fakultas Pertanian, Universitas Gadjah Mada, Indonesia
Wilis Ari Setyati  -  Departemen Ilmu Kelautan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro, Indonesia
Rini Pramesti  -  Departemen Ilmu Kelautan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro, Indonesia
Received: 19 Jul 2017; Published: 19 Jul 2017.
Open Access Copyright (c) 2017 Jurnal Kelautan Tropis

Citation Format:

Lactic acid bacteria are groups of bacteria that have been commonly used as probiotics with functional targets controlling pathogens. In this study, BAL were isolated from penaeid shrimp intestinum was captured in natural waters and were tested of antibacterial activity against 3 types of pathogenic vibrio bacteria in shrimp. The shrimp samples were taken from the North Coast of Java (Situbondo and Semarang) and the South coast of Java (Cilacap). BAL isolation was done by pour plate method using MRS medium enriched with CaCO3. The antibacterial activity test was performed by using paper diffusion method using V. harveyi, V. anguilarum and V. parahaemolyticus as bacteria indicator. The isolation result obtained 224 isolates of BAL intestinal of penaeid shrimp. The active BAL isolates against V. Harveyi, V. anguilarum and V. parahaemolyticus were 59, 83 and 66 isolates, respectively. Isolates are active against 3 types, 2 types and 1 type of Vibrio were 51, 25 and 11 isolates respectively.



Bakteri asam laktat merupakan kelompok bakteri yang telah banyak digunakan sebagai probiotik dengan target fungsional mengendalikan pathogen. Pada penelitian ini telah dilakukan isolasi BAL dari intestinum udang penaeid yang ditangkap di perairan alam dan pengujian aktivitas antibakteri terhadap 3 jenis bakteri vibrio pathogenik pada udang. Sampel udang diambil dari Pantai Utara Jawa (Situbondo dan Semarang) dan pantai Selatan Jawa (Cilacap). Isolasi BAL dilakukan dengan metode taburan menggunakan medium MRS yang diperkaya dengan CaCO3. Uji aktivitas antibakteri dilakukan dengan paper diffusion method menggunakan bakteri uji V. harveyi, V. anguilarum dan V. parahaemolyticus. Hasil isolasi diperoleh 224 isolat BAL intestinal udang penaeid. Isolat BAL yang aktif terhadap V. Harveyi, V. anguilarum dan V. parahaemolyticus berturut-turut 59, 83 dan 66 isolat. Isolat yang aktif terhadap 3 jenis, 2 jenis dan 1 jenis Vibrio uji berurut-turut 51, 25 dan 11 isolat.

Keywords: lactic acid bacteria; antibacteria; vibrio; penaeid shrimp; bakteri asam laktat; antibakteri; vibrio; udang penaeid

Article Metrics:

  1. Alakomi, H. L., Skytta, E., Saarela, M., Mattila-Sandholm, T., Latva-Kala, K. Aguirre-Guzmán, G., M., Lara-Flores, J. G., Sánchez-Martínez, A. I. and Campa-Córdova, 2012, The Use of Probiotics in Aquatic Organisms: A review, African Journal of Microbiology Research, 6 : 4845-4857
  2. Balcazar, J. L., de Blas, I., Ruiz-Zarzuela, I., Cunningham, D., Vendrell, D., Mu´zquiz, J. L., 2006, The Role of Probiotics in Aquaculture, Veterinary Microbiology, 114 : 173–186
  3. Balcázar, J., Decamp, O., Vendrell, D., De Blas, I. and Ruiz-Zarzuela, I., 2006, Health and Nutritional Properties of Probiotics in Fish and Shellfish, Microbial Ecology in Health and Disease,18 : 65-70
  4. Bogale, A. T. and Prapulla, S.G., 2015, Studies on Amylase from Probiotic Lactic Acid Bacteria, International Journal of Modern Chemistry and Applied Science, 2(4) : 211-214
  5. Bourouni, O. C., Bour, M. E., Mraouna, R., Abdennaceur, H., Boudabous, A., 2007, Preliminary Selection Study of Potential Probiotic Bacteria from Aquacultural Area in Tunisia, Annals of Microbiology, 57 : 185-190
  6. Cruz, P.M., Ibanez, A. L., MonroyHermosillo, O. A., and Saad, H. C. R., 2012, Use of Probiotics in Aquaculture, ISRN Microbiology, Article ID 916845, 13 pages, http://dx.doi.org/10.5402/2012/916845
  7. da Silva, B.C., Vieira, F.N., Mouriño, J.L.P., Ferreira, G.S., Seiffert, W.Q., 2013, Salts of Organic Acids Selection by Multiple Characteristics for Marine Shrimp Nutrition, Aquaculture, 384–387 : 104–110
  8. De Schryver P, Defoirdt T, Sorgeloos P (2014) Early Mortality Syndrome Outbreaks: A Microbial Management Issue in Shrimp Farming? PLoS Pathogen 10(4): e1003919. https://doi.org/10.1371/journal.ppat.1003919
  9. Drancourt, M. and Raoult, D., 2005, Sequence-Based Identification of New Bacteria: a Proposition for Creation of an Orphan Bacterium Repository, Journal of Clinical Microbiology.,43 (9) : 4311–4315
  10. Farzanfar, A., 2006, The use of Probiotic in Shrimp Aquaculture, FEMS Immunology & Medical Microbiology 48:149-158
  11. Heenatigala, P. P. M. & Fernando, M. U. L., 2016, Occurrence of Bacteria Species Responsible for Vibriosis in Shrimp Pond Culture Systems in Sri Lanka and Assessment of the Suitable Control Measures, Sri Lanka Journal of Aquatic Science, 21(1) : 1–17
  12. Hong, X. P., Xu, D., Zhuo, Y., Liu H. Q., Lu, L. Q., 2016, Identification and pathogenicity of Vibrio parahaemolyticus isolates and immune responses of Penaeus (Litopenaeus) vannamei (Boone), Journal of Fish Disease, 39(9):1085-1097
  13. Kosin, B. and Rakshit, S. K., 2006, Microbial and Processing Criteria for Production of Probiotics: A Review , Food Technology and Biotechnology, 44 (3) : 371–379
  14. Ljungh, A, and Wadström, T., 2006, Lactic Acid Bacteria as Probiotics, Current Issues in Intestinal Microbiology, 7(2) : 73-89
  15. Maeda, M. 1., Shibata, A., Biswas, G., Korenaga, H., Kono, T., Itami, T., Sakai, M., 2014, Isolation of Lactic Acid Bacteria from Kuruma Shrimp (Marsupenaeus japonicus) Intestine and Assessment of Immunomodulatory Role of a Selected Strain as Probiotic, Marine Biotechnology (NY),16(2) : 181-92
  16. Mine, S., & Boopathy, R., 2011, Effect of organic acids on shrimp pathogen, Vibrio harveyi, Current Microbiology, 63(1):1-7
  17. Ng, W., Koh, C., Teoh, C., Romano, N., 2015, Farm-Raised Tiger Shrimp, Penaeus Monodon, Fed Commercial Feeds With Added Organic Acids Showed Enhanced Nutrient Utilization, Immune Response And Resistance To Vibrio Harveyi Challenge, Aquaculture, 449 : 69-77
  18. Nikoskelainen, S., Salminen, S., Bylund, G., Ouwehand, A.C., 2001a., Characterization of the Properties of Human and Dairy Derived Probiotics for Prevention of Infectious Diseases in Fish. Applied and Environmental Microbiology, 67 : 2430-2435
  19. Noordiana, N., Fatimah, A. B. and Mun, A. S., 2013, Antibacterial Agents Produced by Lactic Acid Bacteria Isolated from Threadfin Salmon and Grass Shrimp, International Food Research Journal, 20(1) : 117-124
  20. Rattanachaikunsopon, P., and Phumkhachorn, P., 2010, Lactic Acid Bacteria : their Antimicrobial Compounds and their Uses in Food Production, Annals of Biological Research, 1 : 218-228
  21. Ricke, S. C., 2003, Perspectives on the Use of Organic Acids and Short Chain Fatty Acids as Antimicrobials, Poultry Science, 82 : 632–639
  22. Romano, N., Koh, C., Ng, W. K., 2015, Dietary Microencapsulated Organic Acids Blend Enhances Growth, Phosphorus Utilization, Immune Response, Hepatopancreatic Integrity and Resistance Against Vibrio harveyi in White Shrimp, Litopenaeus vannamei, Aquaculture, 435 : 228–236
  23. Sha, Y., Wang, L., Liu, M., Jiang, K., Xin, F., Wang, B., 2016, Effects of Lactic Acid Bacteria and the Corresponding Supernatant on the Survival, Growth Performance, Immune Response and Disease Resistance of Litopenaeus vannamei, Aquaculture, 452 : 28-36
  24. Shakila, R. J., Saravanakumar, R., Vyla, S. A. P., Jeyasekaran, G., Jasmine, G. I., 2006, Antagonistic Activity of the Gut Microflora Isolated from Farmed Tiger Shrimp (Penaeus monodon), Asian Fisheries Science,19 : 247-255
  25. Soto-Rodriguez, S. A., Gomez-Gil, B., Lozano, R., 2010, ‘Bright-red’ syndrome in Pacific white shrimp Litopenaeus vannamei is caused by Vibrio harveyi, Diseases Of Aquatic Organisms, 92: 11–19
  26. Sunaryanto, R. dan Tarwadi, 2015, Isolasi dan Karakterisasi Bakteriosin yang Dihasilkan oleh oleh lactobacillus lactis dari Sedimen Laut, JPB Kelautan dan Perikanan, 10 (1 ) : 11–18
  27. Suskovic, J., Kos, B., Beganovic, J., Pavunc, A. L., Habjanic, K., Matosic, S., 2010, Antimicrobial Activity–the Most Important Property of Probiotic and Starter Lactic, Food Technology and Biotechnology, 48 : 296–307
  28. Vesterlund, S., Karp, M., Salminen, S. and Ouwehand, A. C., 2006, Staphylococcus aureus Adheres To Human Intestinal Mucus but can be Displaced by Certain Lactic Acid Bacteria. Microbiology, 152 : 1819–1826
  29. Vieira, F. P., Jatobá A., Mouriño, J. L. P., Vieira, E. A., Soares, M., da Silva, B. C., Seiffert, W. Q., Martins, M. L. and Vinatea, L. A., 2013, In vitro selection of Bacteria with Potential for Use as Probiotics in Marine Shrimp Culture, Pesquisa Agropecuária Brasileira, 48(8) : 998-1004
  30. Zhitnitsky, D., Rose, J., & Lewinson, O. (2017). The highly synergistic, broad spectrum, antibacterial activity of organic acids and transition metals. Scientific Reports, 7, 44554. http://doi.org/10.1038/srep 44554
  31. Zhou, X., Wang, Y., 2003. Probiotic in aquaculture-Benefits to the Health, Technological Applications and Safety, dalam Health and Environment in Aquaculture, E. Carvalho (ed.), InTech Europe, Croatia. p.215-226

Last update: 2021-04-17 01:15:02

No citation recorded.

Last update: 2021-04-17 01:15:03

No citation recorded.