1Department of Postgraduate Biomedical Science, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia
21. Department of Postgraduate Biomedical Science, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia
32. Department of Surgery, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia
4 Department of Pediatrics, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia
BibTex Citation Data :
@article{JBTR29306, author = {Pinandhito Latukolan and Eko Setiawan and Sri Priyantini}, title = {Hypoxia-Mesenchymal Stem Cell Exosomes As A Novel Therapy Targeting Socs3/Stat3 Signaling In Androgenic Alopecia}, journal = {Journal of Biomedicine and Translational Research}, volume = {12}, number = {2}, year = {2026}, keywords = {Androgenic Alopecia; Mesenchymal Stem Cells; Exosomes; Cell Hypoxia; Signal Transduction}, abstract = { Background : Androgenetic alopecia (AGA) is the most common form of hair loss, characterized by progressive follicular miniaturization associated with dysregulation of suppressor of cytokine signaling 3 (SOCS3) and signal transducer and activator of transcription 3 (STAT3). Current therapies, such as minoxidil, provide limited efficacy and are often accompanied by side effects. Exosomes derived from hypoxia-conditioned mesenchymal stem cells (EH-MSCs) offer regenerative, immunomodulatory, and anti-inflammatory effects that may represent a novel therapeutic approach. Objective : To evaluate the therapeutic potential of EH-MSC exosomes in regulating SOCS3 and STAT3 signaling in a dihydrotestosterone-induced AGA mouse model. Methods : An in vivo randomized post-test–only control group design was conducted using thirty-four male C57BL/6 mice divided into five groups: healthy control (K1), DHT-induced AGA + saline (K2), DHT-induced + minoxidil 5% (K3), DHT-induced + EH-MSC exosomes 100 μg/kg (K4), and DHT-induced + EH-MSC exosomes 200 μg/kg (K5). Exosomes were isolated from hypoxia-preconditioned umbilical cord MSCs using tangential flow filtration and validated with CD63/CD9 markers by flow cytometry. SOCS3 and STAT3 expression levels were analyzed by qRT-PCR. Statistical tests included Shapiro–Wilk, Levene’s, one-way ANOVA, and post hoc analysis. Results : EH-MSC exosomes significantly modulated SOCS3 and STAT3 expression in a dose-dependent manner. The high-dose EH-MSC group (K5) restored SOCS3 expression (1.25 ± 0.18 pg/mL) to levels comparable with healthy controls (1.00 ± 0.00 pg/mL) and suppressed STAT3 expression (0.89 ± 0.15 pg/mL) toward normal values. Minoxidil only partially improved SOCS3 (0.49 ± 0.16 pg/mL) and STAT3 (2.32 ± 0.19 pg/mL) expression compared to untreated AGA controls. Conclusion : Exosomes derived from hypoxia-preconditioned MSCs demonstrated superior immunomodulatory effects compared to minoxidil by enhancing SOCS3 and suppressing STAT3 expression, thereby restoring follicular homeostasis in AGA. These findings suggest EH-MSC exosomes as a promising exosome-based therapeutic strategy for androgenetic alopecia, with potential implications for clinical nursing practice in regenerative dermatology. }, issn = {2503-2178}, pages = {113--122} doi = {10.14710/jbtr.v12i2.29306}, url = {https://ejournal2.undip.ac.id/index.php/jbtr/article/view/29306} }
Refworks Citation Data :
Background: Androgenetic alopecia (AGA) is the most common form of hair loss, characterized by progressive follicular miniaturization associated with dysregulation of suppressor of cytokine signaling 3 (SOCS3) and signal transducer and activator of transcription 3 (STAT3). Current therapies, such as minoxidil, provide limited efficacy and are often accompanied by side effects. Exosomes derived from hypoxia-conditioned mesenchymal stem cells (EH-MSCs) offer regenerative, immunomodulatory, and anti-inflammatory effects that may represent a novel therapeutic approach.
Objective: To evaluate the therapeutic potential of EH-MSC exosomes in regulating SOCS3 and STAT3 signaling in a dihydrotestosterone-induced AGA mouse model.
Methods: An in vivo randomized post-test–only control group design was conducted using thirty-four male C57BL/6 mice divided into five groups: healthy control (K1), DHT-induced AGA + saline (K2), DHT-induced + minoxidil 5% (K3), DHT-induced + EH-MSC exosomes 100 μg/kg (K4), and DHT-induced + EH-MSC exosomes 200 μg/kg (K5). Exosomes were isolated from hypoxia-preconditioned umbilical cord MSCs using tangential flow filtration and validated with CD63/CD9 markers by flow cytometry. SOCS3 and STAT3 expression levels were analyzed by qRT-PCR. Statistical tests included Shapiro–Wilk, Levene’s, one-way ANOVA, and post hoc analysis.
Results: EH-MSC exosomes significantly modulated SOCS3 and STAT3 expression in a dose-dependent manner. The high-dose EH-MSC group (K5) restored SOCS3 expression (1.25 ± 0.18 pg/mL) to levels comparable with healthy controls (1.00 ± 0.00 pg/mL) and suppressed STAT3 expression (0.89 ± 0.15 pg/mL) toward normal values. Minoxidil only partially improved SOCS3 (0.49 ± 0.16 pg/mL) and STAT3 (2.32 ± 0.19 pg/mL) expression compared to untreated AGA controls.
Conclusion: Exosomes derived from hypoxia-preconditioned MSCs demonstrated superior immunomodulatory effects compared to minoxidil by enhancing SOCS3 and suppressing STAT3 expression, thereby restoring follicular homeostasis in AGA. These findings suggest EH-MSC exosomes as a promising exosome-based therapeutic strategy for androgenetic alopecia, with potential implications for clinical nursing practice in regenerative dermatology.
Article Metrics:
Last update:
The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Journal of Biomedicine and Translational Research Diponegoro University as publisher of the journal.
Copyright encompasses non-exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations.
Journal of Biomedicine and Translational Research Diponegoro University, the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal. In any way, the contents of the articles and advertisements published in the Journal of Biomedicine and Translational Research Diponegoro University (JBTR) are sole and exclusive responsibility of their respective authors and advertisers.
The Copyright Transfer Form can be downloaded here: [Copyright Transfer Form JBTR]
The copyright form should be signed originally and send to the Editorial Office in the form of original mail, scanned document or fax : Journal of Biomedicine and Translational Research Faculty of Medicine, Diponegoro UniversityJl. Prof. Soedarto, Kampus UNDIP Tembalang, Semarang, Central Java, Indonesia 50275, Telp.: +62-24-8454714, Fax.: +62-24-8454714Email: jbtr@live.undip.ac.id
JBTR by https://ejournal2.undip.ac.id/index.php/jbtr is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View My Stats
