DOI: https://doi.org/10.14710/gjec.2025.26997

Analisis In Silico Interaksi Enzim Esterase dari Enterobacter terhadap Berbagai Turunan Paraben sebagai Kajian Awal Potensi Biodegradasi

*Arina Amalia Putri orcid scopus  -  Biotechnology Study Program, Department of Biology, Faculty of Sciences and Mathematics, Diponegoro University. Jl. Prof. Soedarto No.50275, Tembalang, Tembalang, Semarang, West Java, Indonesia 50275, Indonesia
Zubaidi Bachtiar orcid  -  Department of Bioengineering, Faculty of Engineering, Lombok Institute of Technology, Lombok, Indonesia, Indonesia
Dhea Ferda Pratiwi  -  Biotechnology Study Program, Graduate School, IPB University, Bogor, Indonesia, Indonesia
Husnul Khotimah  -  Microbiology Study Program, Department of Biology, Faculty of Sciences and Mathematics, , Indonesia
Open Access Copyright 2025 Greensphere: Journal of Environmental Chemistry

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Abstract

Paraben banyak digunakan sebagai pengawet pada industri kosmetik dan farmasi karena bersifat antimikroba dan stabil secara kimia.  Namun, senyawa ini tergolong sebagai endocrine disrupting chemicals (EDCs) yang mengganggu sistem hormonal dan berdampak negatif terhadap lingkungan. Residu paraben yang terakumulasi di perairan dan tanah menunjukkan sifat ‘pseudo-persisten’, sehingga diperlukan strategi mitigasi yang efektif. Studi ini menyajikan pendekatan inovatif melalui pemodelan in silico untuk mengeksplorasi potensi enzim esterase dari bakteri golongan Enterobacter dalam mendegradasi tiga turunan paraben: metilparaben, propilparaben, dan butilparaben. Hasil molecular docking menunjukkan bahwa ketiga senyawa mampu berinteraksi dengan enzim esterase, namun Interaksi butilparaben menunjukkan profil paling stabil, ditandai oleh ikatan hidrogen kuat, dominasi ikatan hidrofobik, dan salt bridge yang paling rapat. Temuan dari studi ini menunjukan potensi dari enzim esterase sebagai agen biodegradasi pada senyawa golongan paraben dengan rantai alkil yang lebih kompleks seperti butilparaben.


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Keywords: Enzim esterase, molecular docking, paraben, toksisitas, biodegradasi

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