DOI: https://doi.org/10.14710/gjec.2025.26815

EFEKTIVITAS KRIM FORMULASI TEH HIJAU (Camellia sinensis) SEBAGAI AGEN ANTIBAKTERI TERHADAP PATOGEN KULIT

*Fitra Adi Prayogo orcid scopus  -  Karya Husada University, Indonesia
Heni Wijayanti  -  Biomedical Sciences Program Study, Karya Husada University Semarang, Indonesia
Novita Rahmawati  -  Biomedical Sciences Program Study, Karya Husada University Semarang, Indonesia
Anto Budiharjo orcid  -  Biotechnology Program Study, Diponegoro University, Indonesia
Dyah Wulandari orcid  -  Food Technology Department, Soegijapranata Catholic University, Indonesia
Open Access Copyright 2025 Greensphere: Journal of Environmental Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Citation Format:
Abstract

This research aims to determine the effect of green tea extract cream 6%, 8%, and 10% w/w against Staphylococcus epidermidis and Propionibacterium acne. The method begins with making green tea extract using the maceration method, and qualitative flavonoid, alkaloid, saponin, and tannin tests are carried out. Next, S. epidermidis and P. acnes were rejuvenated on NA media and cultured at 37°C for 24 hours. Both bacteria were then inoculated into different test tubes containing 0.9% NaCl. The suspension was then vortexed, and the turbidity level was equalized with McFarland's solution. Both bacteria were then cultured on NA media, and paper disks that had previously been soaked in clindamycin (control +) and 0%, 6%, 8%, and 10% green tea extract cream were placed on the agar media. The culture was then incubated for 24 hours at 37°C, and the inhibition zone was checked with a caliper. Results show that green tea extract has positive tests for flavonoids, alkaloids, saponins, and tannins. The optimum concentration of extract cream on antibacterial activity against S. epidermidis and P. acne is 10%. Inhibition zone comparison showed a significant difference between the diameter of the inhibition zone of S. epidermidis and P. acne, p = 0,00485 (p < 0,05), which means that green tea extract cream is better at fighting P. acne.

Fulltext View|Download
Keywords: Camellia sinensis; antiacne; Staphylococcus epidermidis; Propionibacterium acne

Article Metrics:

Article Info
Section: Articles
Language : ID
Recent articles
Fabrikasi, Karakterisasi, dan Uji Antibakteri Nanopartikel Triterpenoid dari Daun Sambung Nyawa (Gynura procumbens) EFEKTIVITAS KRIM FORMULASI TEH HIJAU (Camellia sinensis) SEBAGAI AGEN ANTIBAKTERI TERHADAP PATOGEN KULIT Analisis In Silico Interaksi Enzim Esterase dari Enterobacter terhadap Berbagai Turunan Paraben sebagai Kajian Awal Potensi Biodegradasi More recent articles
  1. Nurjanah, B. Efrata Aprilia, A. Fransiskayana, M. Rahmawati, and T. Nurhayati, “Senyawa Bioaktif Rumput Laut dan Ampas Teh sebagai Antibakteri dalam Formula Masker Wajah,” JPHPI, vol. 21, no. 2, pp. 304–316, 2018, doi: https://doi.org/10.17844/jphpi.v21i2.23086
  2. L. H. Muharram, F. N. Syaputri, W. Pertiwi, and R. F. Saputri, “Aktivitas Antibakteri Ekstrak Bawang Hitam Variasi Waktu Aging Terhadap Pencegahan Dysbiosis Kulit Penyebab Jerawat,” J. Sains dan Kesehat., vol. 4, no. 2, pp. 181–188, 2022, doi: 10.25026/jsk.v4i2.1035
  3. R. Liao, T. Parker, K. Bellerose, D. Vollmer, and X. Han, “A Green Tea Containing Skincare System Improves Skin Health and Beauty in Adults : An Exploratory Controlled Clinical Study,” Cosmetics, vol. 9, no. 96, 2022, doi: https://doi.org/10.3390/cosmetics9050096
  4. M. A. K. Parvez et al., “Antibacterial activities of green tea crude extracts and synergistic effects of epigallocatechingallate (EGCG) with gentamicin against MDR pathogens,” Heliyon, vol. 5, no. 7, Jul. 2019, doi: 10.1016/j.heliyon.2019.e02126
  5. R. Yanuarti, S. Sartini, and F. Nainu, “Green tea extract-mediated augmentation of imipenem antibacterial activity against Enterobacter cloacae clinical isolates,” Pharmaciana, vol. 11, no. 1, p. 133, Mar. 2021, doi: 10.12928/pharmaciana.v11i1.16874
  6. R. Amelia, P. Sudomo, and L. Widasari, “Perbandingan Uji Eefektivitas Ekstrak Teh Hijau (Camellia sinensis) sebagai Anti Bakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli Secara Invitro,” Bina Widya, vol. 23, no. 4, 2012
  7. N. Noriko, “Potensi Daun Teh (Camellia sinensis) dan Daun Anting-anting Acalypha indica L. dalam Menghambat Pertumbuhan Salmonella typhi,” 2013
  8. S. Liu, Q. Zhang, H. Li, Z. Qiu, and Y. Yu, “Comparative Assessment of the Antibacterial Efficacies and Mechanisms of Different Tea Extracts,” Foods, vol. 11, no. 4, 2022, doi: 10.3390/foods11040620
  9. M. I. Prasanth, B. S. Sivamaruthi, C. Chaiyasut, and T. Tencomnao, “A Review of the Role of Green Tea ( Camellia sinensis ) in Antiphotoaging, Stress Resistance, Neuroprotection, and Autophagy,” Nutr. Rev., vol. 11, no. 474, 2019, doi: 10.3390/nu11020474
  10. C. Lalita and G. Shalini, “Creams: A Review on Classification, Preparation Methods, Evaluation and its Applications,” J. Drug Deliv. Ther., vol. 10, no. 5, pp. 281–289, 2020
  11. R. A. Hidayati, A. Kristijono, and A. Muadifah, “Uji Aktivitas Antibakteri Sediaan Gel Hand Sanitizer Ekstrak Kulit Buah Jengkol (Archidendron pauciflorum (Benth.) Nielsen) terhadap Bakteri Escherichia coli,” J. Sains dan Kesehat., vol. 3, no. 2, pp. 165–176, Apr. 2021, doi: 10.25026/jsk.v3i2.259
  12. J. O. Obwoge, J. K. Kinyua, D. W. Kariuki, and G. N. Magoma, “Phytochemical Screening And Antimicrobial Studies Of Green, Orthodox And Black Kenyan Tea,” JAGST, vol. 16, no. 3, pp. 82–93, 2014
  13. S. Samadi and F. R. Fard, “Biocatalysis and Agricultural Biotechnology Phytochemical properties , antioxidant activity and mineral content ( Fe , Zn and Cu ) in Iranian produced black tea , green tea and roselle calyces,” Biocatal. Agric. Biotechnol., vol. 23, no. 101472, 2020, doi: 10.1016/j.bcab.2019.101472
  14. O. Agral, P. Yamlean, and H. S. Supriati, “Sediaan Krim Anti Inflamasi Getah Tanaman Patah Tulang (Euphorbia tirucalli L),” Pharmacon, vol. 2, no. 03, pp. 5–7, 2013
  15. D. P. Sukamdi, A. Zahrah, and S. Harimurti, “Optimization of Antioxidant Gel Formula Green Tea Leaf Extract ( Camellia sinensis L .) With Virgin Coconut Oil ( VCO ),” Proc. Sel. Pap. Int. Conf. Heal. Sci. Environ. (ICHSE 2023), Adv. Biol. Sci. Res., vol. 39, 2024, doi: 10.2991/978-94-6463-431-0
  16. F. A. Prayogo, “Efek Ektstrak Etanol 96 % Akar Kalembak ( Rheum officinale ) Terhadap Isolat Bakteri dari Pasien Ulkus Diabetikum,” J. Dunia Farm., vol. 9, no. 22, pp. 133–144, 2025, doi: 10.33085/jdf.v9i2.6433
  17. F. Kalalinia et al., “Topical green tea formulation with anti-hemorrhagic and antibacterial effects,” Iran J Basic Med Sci, vol. 23, no. 12, pp. 1085–1090, 2020, doi: 10.22038/ijbms.2020.41397.9782
  18. J. Anand, B. Upadhyaya, P. Rawat, and N. Rai, “Biochemical characterization and pharmacognostic evaluation of purified catechins in green tea ( Camellia sinensis ) cultivars of India,” 3 Biotech, vol. 5, pp. 285–294, 2015, doi: 10.1007/s13205-014-0230-0
  19. S. R. Mita, P. Husni, N. A. Putriana, R. Maharani, R. P. Hendrawan, and D. A. Dewi, “A Recent Update on the Potential Use of Catechins in Cosmeceuticals,” 2024
  20. D. Febriyanti, E. Halimatushadyah, D. A. Waluyo, and K. Rahma, “Formulasi Dan Uji Aktivitas Krim Antijerawat Ekstrak Daun Belimbing Wuluh ( Averrhoa bilimbi L .) Terhadap Bakteri Staphylococcus epidermidis,” no. 1, pp. 129–140, 2023
  21. L. Zeng, M. Ma, C. Li, and L. Luo, “Stability of tea polyphenols solution with different pH at different temperatures,” Int. J. Food Prop., vol. 20, no. 1, pp. 1–18, 2017, doi: 10.1080/10942912.2014.983605
  22. M. C. M. Pratasik, P. V. Y. Yamlean, and W. I. Wiyono, “Formulasi dan Uji Stabilitas Fisik Sediaan Krim Ekstrak Etanol Daun Sesewanua (Clerodendron squamatum Vahl.),” Pharmacon, vol. 8, no. 2, pp. 261–267, 2019
  23. D. Dominica and D. Handayani, “Formulasi dan Evaluasi Sediaan Lotion dari Ekstrak Daun Lengkeng (Dimocarpus Longan) sebagai Antioksidan,” J. Farm. dan Ilmu Kefarmasian Indones., vol. 6, no. 1, pp. 1–7, 2019
  24. K. Kartini, B. M. Winarjo, E. W. Fitriani, and R. Islamie, “Formulation and pH-Physical Stability Evaluation of Gel and Cream of Plantago major Leaves Extract,” Media Pharm. Indones., vol. 1, no. 3, 2017
  25. L. Ouchari, A. Boukeskasse, B. Bouizgarne, and Y. Ouhdouch, “Antimicrobial potential of actinomycetes isolated from the unexplored hot Merzouga desert and their taxonomic diversity,” Biol. Open, vol. 8, no. bio035410, 2019, doi: 10.1242/bio.035410
  26. A. Wulandari, Y. Farida, and S. Taurhesia, “Perbandingan Aktivitas Ekstrak Daun Kelor Dan Teh Hijau Serta Kombinasi Sebagai Antibakteri Penyebab Jerawat,” J. Fitofarmaka Indones., vol. 7, no. 2, pp. 23–29, 2020, doi: 10.33096/jffi.v7i2.535
  27. M. Miklasinska-Majdanik, K. Małgorzata, R. D. Wojtyczka, D. Idzik, and T. J. Wasik, “Phenolic Compounds Diminish Antibiotic Resistance of Staphylococcus Aureus Clinical Strains,” Int J Env. Res Public Heal., vol. 15, no. 10, p. 2321, 2018, doi: 10.3390/ijerph15102321
  28. O. Pramiastuti, N. Hidayah, E. Istriningsih, and G. R. Fisrty, “Potensi Antibakteri Daun Kersen Muntingia calabura L .) Dan Daun Teh Hijau ( Camellia sinensis L .) Terhadap Staphylococcus epidermis JURNAL FARMASI & SAINS INDONESIA,” J. Farm. dan Sains Indones., vol. 6, no. 2, pp. 131–138, 2023, doi: 10.52216/jfsi.vol6no2p131-138
  29. T. J. Hossain, “Methods for screening and evaluation of antimicrobial activity : A review of protocols , advantages , and limitations,” Eur. J. Microbiol. Immunol., vol. 14, no. 2, pp. 97–115, 2024, doi: 10.1556/1886.2024.00035
  30. R. M. Alkufeidy, L. A. Altuwijri, N. S. Aldosari, N. Alsakabi, and T. M. Dawoud, “Antimicrobial and synergistic properties of green tea catechins against microbial pathogens,” J. King Saud Univ. - Sci., vol. 36, no. 8, p. 103277, 2024, doi: 10.1016/j.jksus.2024.103277
  31. N. Widyaningrum, “Development of Anti Acne Cream ( w / o / w Multiple Emulsion ) Containing Green Tea Leaf Waste,” Sains Med., vol. 8, no. 2, pp. 74–78, 2017
  32. Q. Hafizah, L. Permatasari, and N. R. I. Muchlishah, “Faktor-Faktor yang mempengaruhi Aktivitas Terhadap Bakteri Staphylococcus aureus,” J. Kesehat. TAMBUSAI, vol. 5, no. 2, pp. 3829–3836, 2024
  33. W. C. Reygaert, “Green Tea Catechins: Their Use in Treating and Preventing Infectious Diseases.,” Biomed Res. Int., vol. 2018, p. 9105261, 2018, doi: 10.1155/2018/9105261
  34. S. Saric, M. Notay, and R. K. Sivamani, “Green Tea and Other Tea Polyphenols: Effects on Sebum Production and Acne Vulgaris.,” Antioxidants (Basel, Switzerland), vol. 6, no. 1, Dec. 2016, doi: 10.3390/antiox6010002

Last update:

No citation recorded.

Last update:

No citation recorded.