DOI: https://doi.org/10.14710/genres.v3i2.19145

Analisis Residu Enrofloksasin dalam Daging dan Telur Ayam

*Evieta Rohana  -  Program Studi Farmasi Fakultas Kedokteran Universitas Diponegoro, Indonesia
Wimzy Rizqy Prabhata  -  Program Studi Farmasi Fakultas Kedokteran Universitas Diponegoro, Indonesia
Received: 3 Jul 2023; Revised: 9 Oct 2023; Accepted: 12 Oct 2023; Available online: 16 Oct 2023; Published: 16 Oct 2023.
Open Access Copyright 2023 Generics: Journal of Research in Pharmacy

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Abstract
Salah satu antibiotik golongan fluorokuinolon yang banyak digunakan pada hewan ternak adalah enrofloksasin. Penggunaan pada hewan ternak yang tidak terkontrol dapat mengakibatkan percepatan resistensi mikroba. Salah satu upaya kontrol dan perlindungan masyarakat dapat dilakukan dengan menganalisis residu enrofloksasin dalam bahan makanan yang sering dikonsumsi masyarakat, yaitu daging dan telur ayam. Perlu dilakukan pengembangan metode karena residu enrofloksasin dalam sampel tersebut jumlahnya sedikit. Metode analisis yang digunakan harus memiliki sensitifitas tinggi. Metode yang dapat digunakan antara lain adalah HPLC. Analisis residu enrofloksasin pada penelitian ini menggunakan HPLC detektor UV-Vis, dan fase gerak 20 mM asam fosfat dalam air pH 3,0 : asetonitril (83 : 17), kolom C18, panjang gelombang 278 nm, laju alir 1,5 mL/menit. Hasil uji linearitas menunjukkan nilai R2 sebesar 0,9990, akurasi ditunjukkan dengan nilai recovery 98,75% - 101,84%, presisi ditunjukkan dengan nilai RSD 1,83 ; nilai LOD 1,23 µg/mL dan LOQ 4,09 µg/mL. Hasil uji pada sampel daging ayam menunjukkan adanya residu enrofloksasin base sebesar 185,93 µg/kg, sedangkan pada telur ayam sebesar 145,32 µg/kg.
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Keywords: Fluorokuinolon;ternak; asetonitril;asam fosfat

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