DOI: https://doi.org/10.14710/genres.v3i2.19145

Analisis Residu Enrofloksasin dalam Daging dan Telur Ayam

*Evieta Rohana  -  Program Studi Farmasi Fakultas Kedokteran Universitas Diponegoro, Indonesia
Wimzy Rizqy Prabhata  -  Program Studi Farmasi Fakultas Kedokteran Universitas Diponegoro, Indonesia
Received: 3 Jul 2023; Revised: 9 Oct 2023; Accepted: 12 Oct 2023; Available online: 16 Oct 2023; Published: 16 Oct 2023.
Open Access Copyright 2023 Generics: Journal of Research in Pharmacy

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Abstract
Salah satu antibiotik golongan fluorokuinolon yang banyak digunakan pada hewan ternak adalah enrofloksasin. Penggunaan pada hewan ternak yang tidak terkontrol dapat mengakibatkan percepatan resistensi mikroba. Salah satu upaya kontrol dan perlindungan masyarakat dapat dilakukan dengan menganalisis residu enrofloksasin dalam bahan makanan yang sering dikonsumsi masyarakat, yaitu daging dan telur ayam. Perlu dilakukan pengembangan metode karena residu enrofloksasin dalam sampel tersebut jumlahnya sedikit. Metode analisis yang digunakan harus memiliki sensitifitas tinggi. Metode yang dapat digunakan antara lain adalah HPLC. Analisis residu enrofloksasin pada penelitian ini menggunakan HPLC detektor UV-Vis, dan fase gerak 20 mM asam fosfat dalam air pH 3,0 : asetonitril (83 : 17), kolom C18, panjang gelombang 278 nm, laju alir 1,5 mL/menit. Hasil uji linearitas menunjukkan nilai R2 sebesar 0,9990, akurasi ditunjukkan dengan nilai recovery 98,75% - 101,84%, presisi ditunjukkan dengan nilai RSD 1,83 ; nilai LOD 1,23 µg/mL dan LOQ 4,09 µg/mL. Hasil uji pada sampel daging ayam menunjukkan adanya residu enrofloksasin base sebesar 185,93 µg/kg, sedangkan pada telur ayam sebesar 145,32 µg/kg.
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Keywords: Fluorokuinolon;ternak; asetonitril;asam fosfat

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  1. Alquadeib, B. T. (2019) ‘Development and Validation of New HPLC Analytical Method for the Determination of Diclofenac in Tablets’, Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society, 27(1), pp. 66–70. doi: 10.1016/j.jsps.2018.07.020
  2. Beyi, A. F. et al. (2022) ‘Comparisons of Plasma and Fecal Pharmacokinetics of Danofoxacin and Enrofoxacin in Healthy and Mannheimia Haemolytica Infected Calves’, Scientific reports, 12(1), p. 5107. doi: 10.1038/s41598-022-08945-z
  3. Brito, J. C. et al. (2022) ‘Development and Validation of a Rapid and Reliable HPLC-FLD Method for the Quantification of Ciprofloxacin and Enrofloxacin Residues in Zea mays’, Journal of the Brazilian Chemical Society, 33(2), pp. 128–134. doi: 10.21577/0103-5053.20210129
  4. Donepudi, S. and Achanta, S. (2018) ‘Validated HPLC-UV Method for Simultaneous Estimation of Linagliptin and Empagliflozin in Human Plasma’, International Journal of Applied Pharmaceutics, 10(3), pp. 56–61. doi: 10.22159/ijap.2018v10i3.24662
  5. Hu, S. et al. (2019) ‘Using Molecular Desceiptors for Assisted Screening of Heterologous Competitive Antigens to Improve The Sensitivity of ELISA for Detection of Enrofloxacin in Raw Milk’, Journal of Dairy Science, 102(7), pp. 6036–6046. doi: 10.3168/jds.2018-16048
  6. International Council for Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (2005) Validation of Analytical Procedures: Text and Methodology Q2(R1). International Council for Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use
  7. Janecko, N. et al. (2016) ‘Implications of Fluoroquinolone Contamination for The Aquatic Environment-A review’, Environmental Toxicology and Chemistry, 35(11), pp. 2647–2656. doi: 10.1002/etc.3552
  8. Karunarathna, N. B. et al. (2021) ‘Occurrence Of Enrofloxacin And Ciprofloxacin Residues In Broiler Meat Sold In Sri Lanka’, Journal of the National Science Foundation of Sri Lanka, 49(4), pp. 479–492. doi: 10.4038/jnsfsr.v49i4.10113
  9. Kowalska, M. et al. (2022) ‘Management of Validation of HPLC method for Determination of Acetylsalicylic Acid Impurities in a New Pharmaceutical Product’, Scientific Reports, 12(1). doi: 10.1038/s41598-021-99269-x
  10. Lei, X. et al. (2022) ‘lImmunochromatographic Assays for Ultrasensitive and High Specific Determination of Enrofloxacin in Milk, Eggs, Honey, and Chicken Meat’, Journal of Dairy Science, 105(3), pp. 1999–2010. doi: 10.3168/jds.2021-20276
  11. Naseef, H., Moqadi, R. and Qurt, M. (2018) ‘Development and Validation of an HPLC Method for Determination of Antidiabetic Drug Alogliptin Benzoat in Bulk and Tablets’, Journal of Analytical Methods in Chemistry, eCollectio. doi: 10.1155/2018/1902510
  12. Rahman, M. R. T., Fliss, I. and Biron, E. (2022) ‘Insights in the Development and Uses of Alternatives to Antibiotic Growth Promoters in Poultry and Swine Production’, Antibiotics (Basel, Switzerland), 11(6), p. 766. doi: 10.3390/antibiotics11060766
  13. Salama, F. M. et al. (2018) ‘Potentiometric Determination Of Enrofloxacin Using PVC And Coated Graphite Sensors’, Global Drugs and Therapeutics, 3(3), pp. 1–6. doi: 10.15761/GDT.1000146
  14. Santoso, S. W. H., Ardana, I. B. K. and Gelgel, K. T. P. (2020) ‘Prevalensi Colibacillosis pada Broiler yang Diberi Pakan Tanpa Antibitic Growth Promoters’, Indonesia Medicus Veterinus, 9(2), pp. 197–205. doi: 10.19087/imv.2020.9.2.197
  15. Schneider, M. J. (2004) ‘Rapid Fluorescence Screening Assay for Enrofloxacin and Tetracyclines in Chicken Muscle’, Journal of Agricultural and Food Chemistry, 52(26), pp. 7809–7813. doi: 10.1021/jf048464u
  16. Tasneem O., E., Shaza W. A., S. and Mohamed E., A. (2019) ‘Development and Validation of UV-Spectrophotometric method for the Determination of Enrofloxacin in Synthetic form and Veterinary Injectible Dosage forms’, Asian Journal of Pharmaceutical Analysis, 9(1), pp. 11–14. doi: 10.5958/2231-5675.2019.00004.8
  17. The European Commission (2010) ‘Commission regulation (EU) No 37/2010 of 22 December 2009 on Pharmacologically Active Substances and Their Classification Regarding Maximum Residue Limits in Foodstuffs of Animal Origin’, Official Journal of the European Union, 15(L15), pp. 1–72
  18. Tim Penyusun Farmakope Obat Hewan Indonesia (2008) Farmakope Obat Hewan Indonesia. Direktorat. Jakarta
  19. U.S. Food & Drug Administration (2021) 2020 Summary Report on Antimicrobials Sold or Distributed for Use in Food-Producing Animals
  20. Widiyanti, P. M. et al. (2019) ‘Penggunaan Antibiotika Enrofloksasin sebagai Obat Hewan dan Bahaya Residunya terhadap Kesehatan Masyarakat’, WARTAZOA, 29(2), pp. 75–84. doi: 10.14334/wartazoa.v29i2.2015

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