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Efek Serbuk Kunyit dan Kurkumin pada Spermatogenesis Mencit (Mus musculus) yang Diberi Minuman Beralkohol

*Helena Chika Valencia Hanisa  -  Program Studi Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro, Jl. Prof. Soedarto, SH, Tembalang, Semarang 50275, Indonesia, Indonesia
Tyas Rini Saraswati orcid scopus  -  Program Studi Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro, Jl. Prof. Soedarto, SH, Tembalang, Semarang 50275, Indonesia, Indonesia
Silvana Tana  -  Program Studi Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro, Jl. Prof. Soedarto, SH, Tembalang, Semarang 50275, Indonesia, Indonesia
Open Access Copyright 2021 Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology)

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Abstract

Kunyit mengandung senyawa kurkumin yang dapat digunakan sebagai zat antiinflamasi dan membantu memperbaiki sel-sel yang rusak. Tujuan dari penelitian ini menganalisis pengaruh serbuk kunyit dan kurkumin pada jumlah dan ukuran sel spermatogonium; spermatosit primer; dan spermatosit sekunder; bobot testis serta diameter tubulus seminiferus Mus musculus yang diberi minuman beralkohol. Penelitian ini merupakan penelitian eksperimental dengan Rancangan Acak Lengkap (RAL), menggunakan 12 ekor Mus musculus jantan yang dibagi kedalam 4 kelompok perlakuan dan 3 kali ulangan. R0 merupakan kontrol, R1 kontrol alkohol, R2 pemberian serbuk kunyit sebanyak 0,1 mg/hari, R3 pemberian kurkumin sebanyak 0,01 mg/hari. Perlakuan diberikan selama 30 hari. Data penelitian dianalisis menggunakan analysis of variance (ANOVA) pada taraf kepercayaan 95%. Berdasarkan hasil penelitian yang diperoleh dapat disimpulkan bahwa tidak terdapat perbedaan bermakna (p>0.05) pada jumlah spermatogonium dan ukuran sel (spermatogonium, spermatosit primer, dan spermatosit sekunder), namun terdapat perbedaan bermakna pada (P<0,05) pada bobot testis, diameter tubulus seminiferus dan jumlah sel (spermatosit primer, dan spermatosit sekunder).

 

Turmeric contains curcumin compounds that can be used as anti-inflammatory substances and help repair damaged cells. The purpose of this study was to analyze the effect of turmeric powder and curcumin on the number and size of spermatogonia cells; primary spermatocytes; and secondary spermatocytes; testicular weight and diameter of the seminiferous tubules of Mus musculus given alcoholic beverages. This study is an experimental study with a completely randomized design (CRD), using 12 male Mus musculus which were divided into 4 treatment groups and 3 replications. R0 is control, R1 is alcohol control, R2 is 0.1 mg/day of turmeric powder, R3 is 0.01 mg/day of curcumin. The treatment was given for 30 days. The research data were analyzed using analysis of variance (ANOVA) at the 95% confidence level. Based on the results obtained, it can be concluded that there is no significant difference (p>0.05) in the number of spermatogonia and cell size (spermatogonia, primary spermatocytes, and secondary spermatocytes), but there is a significant difference (P<0.05) in testicular weight, diameter of the seminiferous tubules and the number of cells (primary spermatocytes, and secondary spermatocytes).

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Keywords: antiinflamasi; bobot testis; tubulus seminiferus; spermatogenesis
Funding: Universitas Diponegoro under contract SPK: 385-51/UN7.P4.3/PP/2018

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  1. Antari. 2012. Penurunan Jumlah Sel Spermatogeni Setelah Pemberian Alkohol Peroral Secara Kronis Pada Tikus Putih. Prosiding Seminar Nasional Prodi Biologi F. MIPA Udayana. ISBN:978-602-9138-68-9
  2. Aznam, N. 2004. Uji aktivitas antioksidan ekstrak kunyit (Curcuma domestika, Val.). Prosiding Semnas Penelitian, Pendidikan dan Penerapan MIPA. 111-117. Badan Standarisasi Nasional. 1996. SNI 01-4277-1996
  3. Bachri, M. S. 2011. Efek Toksisitas Fraksi Etil Asetat Akar Senggani (Melastoma affine D.Don ) pada Organ Hepar, Ginjal dan Testis. Jurnal Ilmiah Kefarmasian, (1) : 27 – 31
  4. Cichoz-Lach H. 2006. Genetic polymorphism of alcohol dehydrogenase 3 in alcohol liver cirrhosis and in alcohol chronic pancreatitis. Alcohol Alcohol , 41(1): 14-17
  5. Hartono, Rodhi, Soewono, Tri R. 2019. Pengaruh Pemberian Alkohol Peroral Terhadap Nilai Mean Corpuscular Volume, Morfologi Eritrosit Darah Tepi dan Normoblas Sumsum Tulang. Jaringan Laboratorium Medis, 1(1)
  6. Karimi, S., Khorsandi, L., Nejaddehbashi, F. 2019. Protective effects of Curcumin on testicular toxicity induced by titanium dioxide nanoparticles in mice. JBRA assisted reproduction, 23(4) : 344 – 351
  7. Khorsandi L., Mirhoseini M., Mohamadpour M., Orazizadeh M., Khaghani S. 2013. Effect of curcumin on dexamethasone-induced testicular toxicity in mice. Pharm Biol. , 51(2):206-212
  8. Lin, C. 2015. Curcumin dose-dependently improves spermatogenic disorders induced by scrotal heat stress in mice. Food Funct, (6) : 3770-3777
  9. Mahmoudi R., Honarmand Z., Karbalay-Doust S., Jafari-Barmak M., Nikseresht M., Noorafshan A. 2017. Using curcumin to prevent structural impairments of testicles in rats induced by sodium metabisulfite. EXCLI J, (16) : 583 - 592
  10. Magista M, Nuryanti, Archadian, Wahyudi, Ivan A. 2014. Pengaruh Lama Perendaman dan Jenis Minuman Beralkohol Bir dan Tuak terhadap Kekerasan Email Gigi Manusia (In Vitro). Maj. Ked Gi, 21(1): 47 – 55
  11. Mardiati, S. M., Saraswati, T. R., 2014. Buku Penuntun Praktikum Mikroteknik Hewan. Semarang: Universitas Diponegoro
  12. Mu Y., Yan W., Yin T., Yang J. 2016. Curcumin ameliorates high‑fat diet‑induced spermatogenesis dysfunction. Molecular Medicine Reports, (14) : 3588-3594. https://doi.org/10.3892/mmr.2016.5712
  13. Nurrochmad, Arief. 2004. REVIEW: Pandangan Baru Kurkumin dan Aktivitasnya sebagai Antikanker. Biofarmasi, 2(2) : 75-80
  14. Pfeiffer, F., Hohle. S., Solyom, A.M., Metzler, M. 2003. Studies on the stability of turmeric constituens. Journal of Food Engineering, (56) : 257-259
  15. Pietrangelo, A. 2014. The Effects of alcohol on the Body. Medically Reviewed by George Krucik, MD, MBA
  16. Rees, T.J. 2005. The Toxicology of Male Reproduction. Literature Review in Applied Toxicology. Portsmouth University
  17. Widyastuti, W., Mardiati, S. M., Saraswati, T. R., 2014. Pertumbuhan Puyuh (Coturnix coturnix japonica) Setelah Pemberian Tepung Kunyit (Curcuma longa L.) Pada Pakan. Buletin Anatomi dan Fisiologi, 22(2), pp. 12-20

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