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Effect of a 10% Forest Honey Mouth Rinse on Plaque Index Scores in Children Aged 9–12 Years

Dentistry Study Program, Faculty of Medicine, Universitas Sriwijaya, Indonesia

Received: 4 Aug 2026; Revised: 25 Aug 2026; Accepted: 24 Sep 2026; Published: 1 Jun 2027.
Open Access Copyright 2026 Siti Rusdiana Puspa Dewi, Fairuz Mudiah, Budi Asri, Ulfa Yasmin
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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Abstract
Mechanical plaque control through toothbrushing is the primary method of maintaining oral hygiene, while mouthwash is commonly used as an adjunct. Because conventional mouthwashes may cause oral side effects, natural alternatives such as forest honey have gained attention due to their antibacterial properties. This study evaluated the effect of a 10% forest honey mouthwash on dental plaque scores in children aged 9–12 years. A quasi-experimental study with a pretest–posttest control group design was conducted. Participants were assigned to a control group (distilled water) or an intervention group (10% forest honey mouthwash). They rinsed with 10 mL of the assigned solution for 30 seconds, three times daily, for four consecutive days. Plaque scores were recorded before and after the intervention. Data were analyzed using paired and independent t-tests. The intervention group showed a significant reduction in plaque score from 2.22 ± 0.59 to 1.51 ± 0.57 (p < 0.05), whereas no significant change was observed in the control group (p > 0.05). It can be concluded that a 10% forest honey mouthwash effectively reduced dental plaque scores in children aged 9–12 years.
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Keywords: Child; Dental Plaque; Honey; Oral hygiene; Mouthwash.

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Section: Original Research
Language : EN
  1. Sasitharee Nathamtong. Site-Specific Factors Influencing Dental Plaque Accumulation in Gingivitis: A Cross-Sectional Study. Journal of International Dental and Medical Research. 2025:18(3);1221-1227
  2. Gaur S, Agnihotri R. Is dental plaque the only etiological factor in Amlodipine induced gingival overgrowth? A systematic review of evidence. J Clin Exp Dent. 2018 Jun 1;10(6):e610-e619. doi: 10.4317/jced.54715
  3. Korona-Glowniak I, Skawinska-Bednarczyk A, Wrobel R, Pietrak J, Tkacz-Ciebiera I, Maslanko-Switala M, Krawczyk D, Bakiera A, Borek A, Malm A, Mielnik-Blaszczak M. Streptococcus sobrinus as a Predominant Oral Bacteria Related to the Occurrence of Dental Caries in Polish Children at 12 Years Old. Int J Environ Res Public Health. 2022 Nov 15;19(22):15005. doi: 10.3390/ijerph192215005
  4. Dewi SRP, Nabiilah ZY. Potential of Uncaria Gambir Extract Against Glucosyltransferase Enzyme: A Narrative Review. interdental [Internet]. 2026 Apr. 30 [cited 2026 Aug. 3];22(1):141-6. https://doi.org/10.46862/interdental.v22i1.10706
  5. McGrath C, Clarkson J, Glenny AM, Walsh LJ, Hua F. Effectiveness of Mouthwashes in Managing Oral Diseases and Conditions: Do They Have a Role? Int Dent J. 2023 Nov;73 Suppl 2(Suppl 2):S69-S73. doi: 10.1016/j.identj.2023.08.014
  6. Živković JM, Jovanović N, Matić SL, Joković N, Dubroja Krstić A, Mitić V, Stojanović G, Zlatanović Đajić I, Nikolić J, Najdanović J, Mitrović T, Cvetković VJ, Stankov Jovanović V. Honey as a multifunctional natural product with health-promoting potential: comparative insights into antioxidant, antimicrobial, and DNA-protective activities. BMC Complement Med Ther. 2026 Apr 9;26(1):186. doi: 10.1186/s12906-026-05371-w
  7. Mahani, Ferdian PR, Ghibran HM, Herlina AF, Nurhasanah S, Nurjanah N, Elfirta RR, Pribadi A, Amalia RLR, Samudra IM. A report on the physicochemical and antioxidant properties of three Indonesian forest honeys produced by Apis dorsata. Food Chem X. 2025 Jan 3;25:102156. doi: 10.1016/j.fochx.2025.102156
  8. Almasaudi S. The antibacterial activities of honey. Saudi J Biol Sci. 2021 Apr;28(4):2188-2196. doi: 10.1016/j.sjbs.2020.10.017
  9. Kusumastiwi RPO, Octavia A. Effectiveness of oral health education intervention among 9–12-year-old school children. Berita Kedokteran Masyarakat, 2026;42 (01): e26482
  10. Deglovic J, Majtanova N, Majtan J. Antibacterial and Antibiofilm Effect of Honey in the Prevention of Dental Caries: A Recent Perspective. Foods. 2022 Sep 2;11(17):2670. doi: 10.3390/foods11172670
  11. Halimeh, A., Farhad, R. B., Naseh, S., & Karim, N. (2020). Comparative efficacy of honey 12.5% and chlorhexidine 0.2% mouthwashes on the oropharyngeal bacterial colonization in mechanically-ventilated patients: a randomized controlled trial. Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan, 40(3), 440–446. https://doi.org/10.19852/j.cnki.jtcm.2020.03.012
  12. Romário-Silva D, Alencar SM, Bueno-Silva B, Sardi JdCO, Franchin M, Carvalho RDPd, Ferreira TEdSA, Rosalen PL. Antimicrobial Activity of Honey against Oral Microorganisms: Current Reality, Methodological Challenges and Solutions. Microorganisms. 2022; 10(12):2325. https://doi.org/10.3390/microorganisms10122325
  13. Shamsudin NF, Ahmed QU, Mahmood S, Ali Shah SA, Khatib A, Mukhtar S, Alsharif MA, Parveen H, Zakaria ZA. Antibacterial Effects of Flavonoids and Their Structure-Activity Relationship Study: A Comparative Interpretation. Molecules. 2022 Feb 9;27(4):1149. doi: 10.3390/molecules27041149
  14. do Nascimento JB, da Costa JGM. Flavonoids: A Review of Antibacterial Activity Against Gram-Negative Bacteria. Int J Microbiol. 2025 Sep 15;2025:9961121. doi: 10.1155/ijm/9961121
  15. Erttmann, S.F., Gekara, N.O. Hydrogen peroxide release by bacteria suppresses inflammasome-dependent innate immunity. Nat Commun 10, 3493 (2019). https://doi.org/10.1038/s41467-019-11169-x
  16. Grinn-Gofroń A, Kołodziejczak M, Hrynkiewicz R, Lewandowski F, Bębnowska D, Adamski C, Niedźwiedzka-Rystwej P. Antimicrobial Potential of Bee-Derived Products: Insights into Honey, Propolis and Bee Venom. Pathogens. 2025 Aug 6;14(8):780. doi: 10.3390/pathogens14080780
  17. Savitri TM, Sembiring LS, Sugiaman. VK. The effect of forest honey solution on the plaque and gingivitis index in adolescent. Makassar Dental Journal 2022;11(2):204-209 https://doi.org/10.35856/mdj.v11i2.596
  18. Husna SA, Dinar N, Vidyahayati IL. The Effectiveness of Rinsing With 10% Forest Honey Solution on Plaque Accumulation in Fixed Orthodontic Patients. Jurnal Kesehatan Gigi 2025;12(2): 130-137. https://doi.org/10.31983/jkg.v12i1.12146
  19. Abid M, Baig MI, Khurshid A , Kumar A , Farooq S, Iqbal Z. Comparing the Efficacy of Chlorhexidine Mouthwash and Natural Honey in Reducing Plaque and Improving Gingival Health. A Randomized Controlled Trial. Pakistan Journal of Health Sciences 2025;18(3):95-99
  20. Reduan MH, Zulkaflee NS, Sabarudin MA, Mat Baharin NH, Nik Azis NM, Abdul Hayei NA. Effects of a Stingless Bee Honey Mouth Rinse on Dental Plaque Accumulation: A Randomised Clinical Trial. Cureus. 2025 Jun 18;17(6):e86307. doi: 10.7759/cureus
  21. Singhal R, Siddibhavi M, Sankeshwari R, Patil P, Jalihal S, Ankola A. Effectiveness of three mouthwashes - Manuka honey, Raw honey, and Chlorhexidine on plaque and gingival scores of 12-15-year-old school children: A randomized controlled field trial. J Indian Soc Periodontol. 2018 Jan-Feb;22(1):34-39. doi: 10.4103/jisp.jisp_356_17

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