Antibacterial Effect of Coleus scutellarioides Leaf Extract Against the Growth of Streptococcus mutans

Khofifa Arda Anggelina Putri; Siti Rusdiana Puspa Dewi; Ibnu Ajiedarmo; Novita Idayani

doi:10.14710/actodont.34015

DOI: https://doi.org/10.14710/actodont.34015

Antibacterial Effect of Coleus scutellarioides Leaf Extract Against the Growth of Streptococcus mutans

Khofifa Arda Anggelina Putri, Siti Rusdiana Puspa Dewi

, Ibnu Ajiedarmo, Novita Idayani

Dentistry Study Programme, Faculty of Medicine, Universitas Sriwijaya, Indonesia

Received: 3 Aug 2026; Revised: 22 Sep 2026; Accepted: 10 Oct 2026; Available online: 11 Nov 2026; Published: 1 Jun 2027.

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

BibTex Citation Data :

@article{actodont34015,
    author = {Khofifa Putri and Siti Dewi and Ibnu Ajiedarmo and Novita Idayani},
    title = {Antibacterial Effect of Coleus scutellarioides Leaf Extract Against the Growth of Streptococcus mutans},
    journal = {Acta Odontologica Indonesia},
  volume = {0},
    number = {0},
    year = {2027},
    keywords = {Antibacterial agents; Coleus scutellarioides; Dental caries; Phytotherapy; Streptococcus mutans.},
    abstract = {Natural antibacterial agents have attracted considerable attention as safer and more affordable alternatives to synthetic antimicrobials.  Coleus scutellarioides  (miana) leaves contain flavonoids, alkaloids, tannins, and saponins with potential antibacterial properties. This study evaluated the antibacterial activity of  C. scutellarioides  leaf extract against  Streptococcus mutans , the primary causative bacterium of dental caries. An in vitro laboratory experiment with a post-test-only control group design was conducted. Leaf extract was tested at concentrations of 15.62, 31.25, 62.5, 125, and 250 mg/mL. Chlorhexidine 0.2% and dimethyl sulfoxide (DMSO) served as positive and negative controls, respectively. Antibacterial activity was assessed using the disk diffusion method, while the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by broth dilution. The extract showed concentration-dependent antibacterial activity. Mean inhibition zones were 3.80 ± 0.20, 5.25 ± 0.25, 7.50 ± 0.30, and 9.80 ± 0.20 mm at concentrations of 31.25, 62.5, 125, and 250 mg/mL, respectively. No inhibition zone was observed at 15.62 mg/mL or in the negative control. The MIC and MBC were 62.5 mg/mL and 125 mg/mL, respectively. It can be concluded that  Coleus scutellarioides  leaf extract demonstrated antibacterial activity against  Streptococcus mutans  and shows potential as a natural antibacterial agent for caries prevention.},
   issn = {3090-7411},  doi = {10.14710/actodont.34015},
    url = {https://ejournal2.undip.ac.id/index.php/actodont/article/view/34015}
}

Citation Format:

Abstract

Natural antibacterial agents have attracted considerable attention as safer and more affordable alternatives to synthetic antimicrobials. Coleus scutellarioides (miana) leaves contain flavonoids, alkaloids, tannins, and saponins with potential antibacterial properties. This study evaluated the antibacterial activity of C. scutellarioides leaf extract against Streptococcus mutans, the primary causative bacterium of dental caries. An in vitro laboratory experiment with a post-test-only control group design was conducted. Leaf extract was tested at concentrations of 15.62, 31.25, 62.5, 125, and 250 mg/mL. Chlorhexidine 0.2% and dimethyl sulfoxide (DMSO) served as positive and negative controls, respectively. Antibacterial activity was assessed using the disk diffusion method, while the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by broth dilution. The extract showed concentration-dependent antibacterial activity. Mean inhibition zones were 3.80 ± 0.20, 5.25 ± 0.25, 7.50 ± 0.30, and 9.80 ± 0.20 mm at concentrations of 31.25, 62.5, 125, and 250 mg/mL, respectively. No inhibition zone was observed at 15.62 mg/mL or in the negative control. The MIC and MBC were 62.5 mg/mL and 125 mg/mL, respectively. It can be concluded that Coleus scutellarioides leaf extract demonstrated antibacterial activity against Streptococcus mutans and shows potential as a natural antibacterial agent for caries prevention.

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Keywords: Antibacterial agents; Coleus scutellarioides; Dental caries; Phytotherapy; Streptococcus mutans.

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Section: Original Research

Language : EN

In 2027: June Edition (Article in Press)

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Matei AT, Visan AI. Mechanism, Efficacy, and Safety of Natural Antibiotics. Antibiotics. 2025; 14(10):981. https://doi.org/10.3390/antibiotics14100981
Stan D, Enciu AM, Mateescu AL, Ion AC, Brezeanu AC, Stan D, Tanase C. Natural Compounds With Antimicrobial and Antiviral Effect and Nanocarriers Used for Their Transportation. Front Pharmacol. 2021 Sep 6;12:723233. doi: 10.3389/fphar.2021.723233
Aprillia K, Sianturi S. Antibacterial activity of miana (Coleus scutellarioides) against Streptococcus pyogenes and Streptococcus mutans. Bioeksperimen: Jurnal Penelitian Biologi 2026;12(1):72–78. https://doi.org/10.23917/bioeksperimen.v12i1.9152
Prayoga T. Evaluation of antibacterial activity in nanoparticles ointment preparation using ethanol extract of miana leaves (Coleus atropurpureus (L.) Benth). 2019;4(2):69-76
Dewi SR, Arif CI, Tuzzahra AH, Handayani P. The effect of toothpaste containing uncaria gambir roxb. extract in oral health. Journal of Dentomaxillofacial Science 2025;10(2): 54-59 https://doi.org/10.15562/jdmfs.v10i2.1860
Dewi SRP, Nabiilah ZY. Potential of Uncaria Gambir Extract Against Glucosyltransferase Enzyme: A Narrative Review. interdental [Internet]. 2026 Apr. 30 [cited 2026 Aug. 3];22(1):141-6. https://doi.org/10.46862/interdental.v22i1.10706
Kartini. Shevira RA, Setiawan F, Pradana AT, Azminah, Sukweenadhi J, Rosyidah A, Widyowati R. Botanical, phytochemical, and bioactivity characterization of Coleus scutellarioides. Biodiversitas Journal of Biological Diversity 2025;26(7):3623-3633
Shamsudin NF, Ahmed QU, Mahmood S, Ali Shah SA, Khatib A, Mukhtar S, Alsharif MA, Parveen H, Zakaria ZA. Antibacterial Effects of Flavonoids and Their Structure-Activity Relationship Study: A Comparative Interpretation. Molecules. 2022 Feb 9;27(4):1149. doi: 10.3390/molecules27041149
Zheng X, Gallot G. Dynamics of Cell Membrane Permeabilization by Saponins Using Terahertz Attenuated Total Reflection. Biophys J. 2020 Aug 18;119(4):749-755. doi: 10.1016/j.bpj.2020.05.040
Othman L, Sleiman A, Abdel-Massih RM. Antimicrobial Activity of Polyphenols and Alkaloids in Middle Eastern Plants. Front. Microbiol. 2019;10:911. doi: 10.3389/fmicb.2019.00911
Poppolo Deus F, Ouanounou A. Chlorhexidine in Dentistry: Pharmacology, Uses, and Adverse Effects. Int Dent J. 2022 Jun;72(3):269-277. doi: 10.1016/j.identj.2022.01.005
Ishak A, Mazonakis N, Spernovasilis N, Akinosoglou K, Tsioutis C. Bactericidal versus bacteriostatic antibacterials: clinical significance, differences and synergistic potential in clinical practice. J Antimicrob Chemother. 2025 Jan 3;80(1):1-17. doi: 10.1093/jac/dkae380
Woo SH, Sung JM, Park H, Kim J, Kim YJ, Kim TK, Lee H, Choi YS. Inhibitory effect of natural extract mixtures on microbial growth and lipid oxidation of sausages during storage. J Anim Sci Technol. 2023 Jan;65(1):225-243. doi: 10.5187/jast.2022.e92

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