Pengaruh Gel Kombinasi Ekstrak Jintan Hitam (Nigella sativa L.) dan Kulit Manggis (Garcinia mangostana L.) terhadap Pembentukan Kolagen Penyembuhan Luka Ulkus Traumatikus pada Tikus Wistar (Rattus norvegicus)

Annisa Lathifah Zahrah; Nur Permatasari

doi:10.14710/actodont.29472

DOI: https://doi.org/10.14710/actodont.29472

Pengaruh Gel Kombinasi Ekstrak Jintan Hitam (Nigella sativa L.) dan Kulit Manggis (Garcinia mangostana L.) terhadap Pembentukan Kolagen Penyembuhan Luka Ulkus Traumatikus pada Tikus Wistar (Rattus norvegicus)

The Effect of a Combined Gel of Black Cumin (Nigella sativa L.) and Mangosteen Peel (Garcinia mangostana L.) Extracts on Collagen Formation in the Healing of Traumatic Ulcer Wounds in Wistar Rats (Rattus norvegicus)

Annisa Lathifah Zahrah¹ , Nur Permatasari²

¹Dentistry Study Programme, Faculty of Dentistry, Brawijaya University, Indonesia

²Department of Oral Biology, Faculty of Dentistry, Brawijaya University, Indonesia

Received: 21 Sep 2025; Revised: 26 Nov 2025; Accepted: 30 Nov 2025; Available online: 6 Jan 2026; Published: 1 Jun 2026.

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

BibTex Citation Data :

@article{actodont29472,
    author = {Annisa Lathifah Zahrah and Nur Permatasari},
    title = {Pengaruh Gel Kombinasi Ekstrak Jintan Hitam (Nigella sativa L.) dan Kulit Manggis (Garcinia mangostana L.) terhadap Pembentukan Kolagen Penyembuhan Luka Ulkus Traumatikus pada Tikus Wistar (Rattus norvegicus)},
    journal = {Acta Odontologica Indonesia},
  volume = {0},
    number = {0},
    year = {2026},
    keywords = {Collagen; Traumatic Ulcer; Black Cumin; Mangosteen Peel},
    abstract = {A traumatic ulcer is a lesion on oral soft tissues caused by trauma. Treatment with  triamcinolone acetonide  is effective, although prolonged use may result in adverse effects. As an alternative, black cumin and mangosteen peel, which contain  thymoquinone  and  xanthones , can promote collagen formation during the wound healing process. This study aims to determine the effect of a gel containing a combination of black cumin ( Nigella sativa L. ) and mangosteen peel ( Garcinia mangostana L. ) extracts on the formation of collagen in the healing of traumatic ulcers in Wistar rats ( Rattus norvegicus ). A randomized post test only controlled group design method was conducted using 40 Wistar rats with 2 observation times (days 6 and 12). The mice were divided into 5 groups: control groups administered with base gel (K-) and  triamcinolone acetonide  0,1% (K+), treatment groups administered with 40% black cumin (P1), 15% mangosteen peel (P2), and a combination of 20% black cumin and 10% mangosteen peel extract gel (P3). Collagen was analyzed using  Masson's trichrome  staining, followed by statistical analysis with Independent t-Test and One-Way ANOVA. The results showed that the combination gel group had a higher average collagen area on days 6 and 12 compared to the other groups. These findings indicate that the combination gel of black cumin and mangosteen peel extracts promotes collagen formation in the healing of traumatic ulcers in Wistar rats.},
   issn = {3090-7411},   pages = {23--34}  doi = {10.14710/actodont.29472},
    url = {https://ejournal2.undip.ac.id/index.php/actodont/article/view/29472}
}

Citation Format:

Abstract

A traumatic ulcer is a lesion on oral soft tissues caused by trauma. Treatment with triamcinolone acetonide is effective, although prolonged use may result in adverse effects. As an alternative, black cumin and mangosteen peel, which contain thymoquinone and xanthones, can promote collagen formation during the wound healing process. This study aims to determine the effect of a gel containing a combination of black cumin (Nigella sativa L.) and mangosteen peel (Garcinia mangostana L.) extracts on the formation of collagen in the healing of traumatic ulcers in Wistar rats (Rattus norvegicus). A randomized post test only controlled group design method was conducted using 40 Wistar rats with 2 observation times (days 6 and 12). The mice were divided into 5 groups: control groups administered with base gel (K-) and triamcinolone acetonide 0,1% (K+), treatment groups administered with 40% black cumin (P1), 15% mangosteen peel (P2), and a combination of 20% black cumin and 10% mangosteen peel extract gel (P3). Collagen was analyzed using Masson's trichrome staining, followed by statistical analysis with Independent t-Test and One-Way ANOVA. The results showed that the combination gel group had a higher average collagen area on days 6 and 12 compared to the other groups. These findings indicate that the combination gel of black cumin and mangosteen peel extracts promotes collagen formation in the healing of traumatic ulcers in Wistar rats.

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Keywords: Collagen; Traumatic Ulcer; Black Cumin; Mangosteen Peel

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Section: Original Research

Language : ID

In 2026: June Edition (Article in Press)

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