Isolasi khamir penghasil enzim inulinase dari buah kersen (Muntingia calabura) serta pengaruh mikronutrien mangan (Mn) pada produksi enzimnya

Abstract

The purpose of this research is to obtain isolates yeast enzyme-producing inulinase from Muntingia calabura and knowing the effect of adding manganese ions to the production of inulinase enzyme from isolation results. The method to be performed is yeast isolation, yeast growth test and inulinase production test. This research is conducted experimentally using Randomized Complete Block Design (RCBD) factorial pattern with 2 factors and three times repitition. The first factor was yeast isolate. The second factor was manganese concentration level (mM) of 0,5 (M1), 1.5 (M2) and 2.5 (M3). The collected data were analyzed using ANOVA 5% signification (α = 0.05) and completed by the Duncan test. The results of this research there are are 3 isolates of yeast that produce inulinase enzyme were isolated from Muntingia calabura. The maximum production of inulinase produced by yeast K2 and K4 is at the 6th hour of incubation time (logarithmic phase) . Maximum inulinase enzyme production produced by the yeast K2 and K4 at the 6th hour of incubation. ANOVA analysis showed variations in the contribution of manganese had a significant effect on the production of the enzyme Y2 and K4 inulinase at the 6th hour of incubation time. Post Hoc Duncan test showed the concentration of manganese M1 (0.5 mM) was the best treatment and had a significant effect on the inulinase production at the 6th hour of incubation time.