Pengaruh variasi sumber karbon terhadap aktivitas enzim isolat khamir Ep A dari limbah kulit buah nanas madu (Ananas comocus L.)

Abstract

Ep A isolate is a yeast isolated from the waste of honey pineapple (Ananas comosus L.). This isolate has enzyme activity like most yeasts. The purpose of this study was to determine what enzymes were produced by Ep A yeast isolates and to analyze the effect of variations in carbon sources on the enzyme activity. The research methods included qualitative enzymatic testing using selective media for amylase, lipase, cellulase and protease, starter production in YEPG medium (Yeast Extract Peptone Glucose) for 24 hours, growth and enzyme activity using YEPG medium with a variation of 2% carbon sources of glucose, fructose and galactose using YEPG media then glucose replaced with 2% fructose and galactose. Growth was measured by cell dry weight, enzyme activity was analyzed by the DNS method. This research used a factorial completely randomized design (RAL). Data were analyzed using One-Way ANOVA test and Duncan's continued test. The qualitative test results showed that Ep A yeast isolate could only produce amylase enzyme with a clear zone around the colony visible. The highest amylase enzyme activity was produced in the treatment with glucose carbon sources, namely 0.033 U/mL, fructose 0.032 U/mL and galactose 0.031 U/mL. The results of the analysis using the One-Way ANOVA test showed a significant effect (p <0.05) of the variation in the carbon source used. This research shows that Ep A yeast isolate can produce amylase enzymes and amylase activity is influenced by carbon sources.