Pengendalian penyakit hawar (lateblight) pada Kentang (Solanum tuberosum L.) melalui penerapan solarisasi tanah dan aplikasi agen hayati Trichoderma harzianum

Eirene Brugman, Endang Dwi Purbajanti, Eny Fuskhah


DOI: https://doi.org/10.14710/joac.1.2.31-38

Abstract


The purpose of the research was to assess the effectiveness of soil solarization and biological agent Trichoderma harzianum application in controlling lateblight disease caused by Phytophthora infestans on potato. The experiment was assigned in a split plot design with soil solarization as main plot and the density of Trichoderma harzianum as sub-plot. Soil solarization treatment consisted of solarization (A1) and non-solarization (B1). Density of Trichoderma harzianum used is B1(control)= 0g, B2 = 10g (107 cfu/l), B3 = 20g (2 x 108 cfu/l), B4 = 30g (3 x 108 cfu/l) and B5 = 40 g (4 x 108 cfu/l). Soil solarization treatment is carried out by using transparent polyethylene sheets with a thickness of 45 µm. Parameters measured were soil temperature, intensity level of pathogen attack, the rate of infection and total yield. Data were subjected to ANOVA and followed by LSD test. Soil solarization for 4 weeks increase the soil temperature by 7,6oC and significantly increase the production of potato by 14.28%. Application of biological agent Trichoderma harzianum on level B4 30g (3 x 108 cfu /L) gives the best results in reducing the the intensity level of pathogen attack by 69.13% and the rate of disease infection into 1,012 unit/day. The results of this research may provide benefical information in the future for plant protection activities.

   Keywords : P.infestans, Trichoderma harzianum, soil solarization, potato


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References


Al-Hazmi, A.S. and M. TariqJaveed. 2016. Effects of different inoculum densities of Trichoderma harzianum ant Trichoderma viridae againts Meloidogyne javanica on Tomato. Saudi Journal of Biological Sciences. 23 : 288-292.

Barnett, H.L. and B.B. Hunter. 1998. Illustrated Genera of Imperfect Fungi 4th Edition. American Phytopathological Society Press, Minnesota.

Bae, S.J., T.K. Mohanta, J.Y. Chung, M. Ryu, G. Park, S. Shim, S. Hong, H. Seo, D.W. Bae, I. Bae, J. Kim and H. Bae. 2016. Trichoderma metabolites as biological control agents against Phytophthora pathogens. Biological Control 92:128-138.

Candido, Vincenzo, D.A. Trifone, M. Vito, and D. Castronuovo. 2011. Scientia horticulturae weed control and yield response of soil solarization with different plastic films in lettuce. Scientia Horticulturae. 130 (3): 491–97.

Carrieri, F., F. Raimo, A. Pentangelo, and E. Lahoz. 2013. F.poliferatum and F. tricintum as casual agent of pink root of onion bulbs and the effect of soil solarization combined with compost amendment in cotrolling their infections in field. Crop Protection. 43 : 31-37.

Culman, S.W., J.M. Duxbury, J.G. Lauren and J.E.Ã Thies. 2006. Microbial community response to soil solarization in Nepal’s rice wheat cropping system. Crop Protection. 38 : 3359-71.

Gasoni, Laura, K. Nancy, Y. Viviana, Kobayashi, B. Silvana, B. Viviana, and G. Zumelzu. 2008. Effect of soil solarization and biocontrol agents on plant stand and yield ´ Rdoba on table beet.Crop Protection 27: 337–42.

Katan, J. and J.E. DeVay. 1991. Soil Solarization. CRC Press. London.

Kartini dan Widodo. 2000. Pengaruh solarisasi tanah terhadap pertumbuhan Sclerotium rolfsii SACC. dan patogenisitasnya pada kacang tanah. Buletin Hama dan Penyakit Tumbuhan. 12(2) : 53-59

Melero-Vara, J.M., A.M. Prados-Ligero, and M.J. Basallote-Ureba 2000. Comparison of physical, chemical and biological methodsof controlling garlic white rot. J.Plant Pathology. 106 : 581–588.

Nathasia, A.A.V., Abadi, A.L., dan T. Wardiyati. 2014. Uji ketahanan 7 klon tanaman kentang terhadap penyakit hawar daun (Phytopthora infestans (Mont.) de Barry). Jurnal Produksi Tanaman. 1 (6) : 540-548

Nirwanto, H. 2007. Pengantar Epidemi dan Manajemen Penyakit Tanaman. UPN Veteran jawa Timur. Surabaya.

Oka, I.Y. 1993. Pengantar Epidemiologi Penyakit Tanaman. Gajah Mada University Press. Yogyakarta.

Otieno, W., T. Aad, J. Mike, and C.O. Othieno. 2003. Efficacy of soil solarization, Trichoderma harzianum, and coffee pulp amendment against Armillaria Sp.. 22: 325–31.

Purnomo, H. 2010. Pengantar Pengendalian Hayati. ANDI, Yogyakarta.

Porras, M. Ã., C. Barrau, and F. Romero. 2007. Effects of soil solarization and trichoderma on strawberry production. Crop Protection. 26: 782–87.

Romao-Dumareq, A.S., N.J. Talbolt, and C.R. Thornton. 2012. RNA interference of endochitinases in the sugarcanes endophyte Trichoderma virens 223 reduces its fitness as a biocontrol agent of pineapple disease. PloS One 7, e47888

Sastrahidayat, I. R., dan S. Djauhari. 2014. Teknik Penelitian Fitopatologi. Universitas Brawijaya Press. Malang.

Sastrahidayat, I.R. 2011. Tanaman Kentang dan Pengendalian Penyakitnya. UB Press, Malang.

Shofiyani, A. dan G.P. Budi. 2014. Efektifitas solarisasi tanah terhadap penekanan perkembangan jamur fusarium ada lahan tanaman pisang yang terinfeksi. Prosiding Seminar Nasional Hasil-hasil penleitian dan Pengabdian LPPM UMP 2014

Soesanto, L., E. Mugiastuti, dan R.F. Rahayuniati. 2011. Inventarisasi dan identifikasi patogen tular-tanah pada pertanaman kentang di Kabupaten Purbalingga. J. Hortikultura 21 (3) : 254-264

Stapleton, J.J. and J.E. DeVay.1995. Soil Solarization: a Natural Mechanism of Integrated Pest Management. CRC Press, Boca Raton.

Suwandi, W.M., Pradjadinata D., Ruswandi, P., Leksono dan M. Nobuo. 2001. Visualisasi gejala infeksi penyakit dan hama pada tanaman dan ubi kentang varietas granola. BPSB-TPH. Jawa Barat.

Vinale, F., K. Sivasithamparam, L. Ghisalberti, R. Marra, and M. Lorito. 2008. Trichoderma-plant pathogen interactions. Soil Biol. Bhiochemical. 40 : 1-10.

Vos, C.M.F., De Cremer, K., Cammue, B.P.A., and B. Connick. 2015. The toolbox of Trichoderma spp. in the biocontrol of botrys cinerea disease. Mol. Plant Pathology 16 : 400 – 412.

www.bps.go.id (diakses pada 16 April 2016)

Youssef, S.A., A.K. Tartoura, and G.A. Abdelraouf. 2016. Evaluation of Trichoderma harzianum and Serratia proteamaculans effect on disease suppression, stimulation of ROS-scavenging enzymes and improving tomato growth infected by Rhizoctonia solani. Biological Control. 100 :79-86.

Zhang, F., H. Ge, F. Zhang, N. Guo, Y. Wang, L. Chen, X. Ji and C. Li. 2016. Biocontrol Potential of Trichoderma harzianum isolate T-aloe against Sclerotinia sclerotiorum in soybean. Plant Physiology and Biochemistry. 100: 64-74.

Zayed, M. S. 2013. Productivity of pepper crop (Capsicum Annuum L.) as affected by organic fertilizer, soil solarization, and endomycorrhizae. Annals of Agricultural Sciences 58 (2) : 131 – 137




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