1Departemen Ilmu Kelautan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro, Indonesia
2Tropical Marine Biodiversity Laboratorium Terpadu, Universitas Diponegoro, Indonesia
3Natural Product Laboratory, Laboratorium Terpadu, Universitas Diponegoro, Indonesia
BibTex Citation Data :
@article{JKT3559, author = {Agus Trianto and Sri Sedjati and Ocky Karna Radjasa and Rachmat Afriyanto and Sakti Imam Muchlisin and Septhy Kusuma Radjasa and Muhammad Syaifudien Bahry}, title = {Pemanfaatan Jamur Simbion Sponge dalam Bioisomerasi Asam Lemak pada Dedak untuk Menghasilkan Asam Lemak Cis}, journal = {Jurnal Kelautan Tropis}, volume = {21}, number = {2}, year = {2018}, keywords = {Sponge; jamur simbion; tran-cis isomerasi; asam lemak}, abstract = { Utilization of Sponges Symbiotic Fungus in the Bioisomeration of Fatty Acids in Brans to Produce Cis Fatty Acid Trans fatty acids are known to have a negative impact on human health such as triggering the coronary heart and blood vessel related diseases. However, many food and animal feed contain trans fatty acids. Bran, which is widely used as animal feed has high fatty acid content and the majority are trans fatty acids. This study aims to determine the potential of Trichoderma harzanum , a sponge symbiont fungus, in the process of fatty acid bioconversion in bran. Bran was fermented in saline and non-salin condition for 15 days in the room temperature. Both fermented and not bran was extracted with methanol, and the extracts were concentrated with rotary evaporator. Fatty acid extracts were analyzed on the GC with hydrolysis and methylation as pre-treatments. The test results showed the fatty acid composition changed, and the was formation of new fatty acids. The bioisomeration process occurs in the Trans-9-Elaidic acid Methyl esther into Cis-9-Oleic Methyl ester. Cis-9-oleic acid is known as oleic acid which is widely found in olive and sunflowers oil. The fungus has potential as biocatalys for production of cis fatty acid. Asam lemak trans dikenal mempunyai dampak negatif terhadap kesehatan manusia seperti memicu terjadinya penyakit jantung koroner dan penyakit terkait pembuluh darah. Namun asam lemak trans banyak terkandung dalam berbagai jenis bahan makanan maupun pakan ternak diantaranya adalah dedak. Dedak banyak digunakan sebagai pakan ternak mempunyai kandungan asam lemak yang tinggi dan mayoritas adalah asam lemak trans. Penelitian ini bertujuan untuk mengetahui potensi jamur simbion spons Trichoderma harzanum dalam proses biokonversi asam lemak pada dedak. Fermentasi dedak dilakukan dalam kondisi salin dan unsalin selama 15 hari pada suhu ruang. Dedak yang difermentasi maupun tidak kemudian diekstrak dengan metanol kemudian dipekatkan dengan rotari eveporator. Ekstrak asam lemak degan GC dengan pre-treatment meliputi hidrolisis dan metilasi. Hasil uji menunjukan adanya perubahan komposisi dan terbentuknya asam lemak baru. Proses bioisomerasi terjadi pada Trans-9-Elaidic acid Methyl estermenjadi Cis-9-Oleic Methyl ester. Cis-9-oleic acid yang dikenal dengan asam oleat yang banyak terdapat dalam minyak zaitun dan bunga matahari. Jamur T. Harzianum mempunyai potensi sebagai biokatalis untuk memproduksi asam lemak cis. }, issn = {2528-3111}, pages = {121--128} doi = {10.14710/jkt.v21i2.3559}, url = {https://ejournal2.undip.ac.id/index.php/jkt/article/view/3559} }
Refworks Citation Data :
Utilization of Sponges Symbiotic Fungus in the Bioisomeration of Fatty Acids in Brans to Produce Cis Fatty Acid
Trans fatty acids are known to have a negative impact on human health such as triggering the coronary heart and blood vessel related diseases. However, many food and animal feed contain trans fatty acids. Bran, which is widely used as animal feed has high fatty acid content and the majority are trans fatty acids. This study aims to determine the potential of Trichoderma harzanum, a sponge symbiont fungus, in the process of fatty acid bioconversion in bran. Bran was fermented in saline and non-salin condition for 15 days in the room temperature. Both fermented and not bran was extracted with methanol, and the extracts were concentrated with rotary evaporator. Fatty acid extracts were analyzed on the GC with hydrolysis and methylation as pre-treatments. The test results showed the fatty acid composition changed, and the was formation of new fatty acids. The bioisomeration process occurs in the Trans-9-Elaidic acid Methyl esther into Cis-9-Oleic Methyl ester. Cis-9-oleic acid is known as oleic acid which is widely found in olive and sunflowers oil. The fungus has potential as biocatalys for production of cis fatty acid.
Asam lemak trans dikenal mempunyai dampak negatif terhadap kesehatan manusia seperti memicu terjadinya penyakit jantung koroner dan penyakit terkait pembuluh darah. Namun asam lemak trans banyak terkandung dalam berbagai jenis bahan makanan maupun pakan ternak diantaranya adalah dedak. Dedak banyak digunakan sebagai pakan ternak mempunyai kandungan asam lemak yang tinggi dan mayoritas adalah asam lemak trans. Penelitian ini bertujuan untuk mengetahui potensi jamur simbion spons Trichoderma harzanum dalam proses biokonversi asam lemak pada dedak. Fermentasi dedak dilakukan dalam kondisi salin dan unsalin selama 15 hari pada suhu ruang. Dedak yang difermentasi maupun tidak kemudian diekstrak dengan metanol kemudian dipekatkan dengan rotari eveporator. Ekstrak asam lemak degan GC dengan pre-treatment meliputi hidrolisis dan metilasi. Hasil uji menunjukan adanya perubahan komposisi dan terbentuknya asam lemak baru. Proses bioisomerasi terjadi pada Trans-9-Elaidic acid Methyl estermenjadi Cis-9-Oleic Methyl ester. Cis-9-oleic acid yang dikenal dengan asam oleat yang banyak terdapat dalam minyak zaitun dan bunga matahari. Jamur T. Harzianum mempunyai potensi sebagai biokatalis untuk memproduksi asam lemak cis.
Article Metrics:
Last update:
Upon acceptance for publication, authors agree to transfer the copyright of their article to Jurnal Kelautan Tropis, while retaining the right to reuse their work under the terms of the open license applied.
From the date of publication, the copyright for each article is held by Jurnal Kelautan Tropis. This transfer allows the journal to manage, disseminate, and preserve scholarly content in accordance with international standards and open access best practices.
Although copyright is held by the journal, all published articles are made available under the Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA 4.0). Under this license, anyone may:
Copy and redistribute the material in any medium or format
Remix, transform, and build upon the material for any purpose, even commercially
provided that:
Appropriate credit is given to the original author(s) and the source
Indications are made of any changes that were made
Derivative works are distributed under the same license (CC BY-SA 4.0)
While copyright is held by the journal, authors retain important reuse rights. Authors may:
Reuse the published version of their article in future works, including books, compilations, and lectures
Deposit the published version in institutional or subject repositories
Share the article freely, including on personal websites or academic networks
as long as the original publication in Jurnal Kelautan Tropis is cited and the CC BY-SA 4.0 license terms are respected.
Authors must ensure that any third-party content included in the article (e.g., figures, images, datasets) is either original, in the public domain, or licensed for reuse under compatible terms. If specific permissions are required, authors must obtain them prior to submission.
For questions regarding copyright or licensing, please contact the editorial office at: j.kelautantropis@gmail.com
View My Stats
Jurnal Kelautan Tropis is published by Departement of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro under a Creative Commons Attribution-ShareAlike 4.0 International License.