Departemen Ilmu Kelautan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro, Indonesia
BibTex Citation Data :
@article{JKT23892, author = {Askiya Suryani and Delianis Pringgenies and Wilis Ari Setyati}, title = {Potensi Antioksidan dan Karakterisasi Pada Kolagen Teripang (Stichopus horrens)}, journal = {Jurnal Kelautan Tropis}, volume = {27}, number = {3}, year = {2024}, keywords = {Antioksidan; FTIR; HPLC; Kolagen}, abstract = { Stichopus horrens, a marine organism known for its numerous health benefits, particularly in the medical field, was the focus of this study, which aimed to evaluate the collagen characterization and antioxidant potential of this species. An exploratory descriptive method was employed. The S. horrens samples were extracted using NaOH, CH3COOH, and distilled water to obtain collagen. After freeze-drying, the total collagen yield was 39.3%. FTIR analysis revealed the highest collagen peak at Amide A (3280.89 cm ⁻ ¹), indicating O-H stretching. Amino acid analysis using HPLC showed L-Leucine as the highest essential amino acid (10.6%), while L-Methionine was the lowest (0.1%). Among non-essential amino acids, L-Glutamic acid was the highest (25.7%), with L-Cystine as the lowest (0.5%). Antioxidant activity was evaluated using DPPH, ABTS, and FRAP assays. The IC50 values were 91.12 ppm for DPPH and 117.17 ppm for ABTS. The FRAP assay indicated a Trolox equivalent value of 153.90 mmol/g. Antioxidant evaluation via DPPH and ABTS assays showed moderate antioxidant strength, while the FRAP assay indicated strong antioxidant potential. Teripang Stichopus horrens, organisme laut yang memiliki berbagai manfaat kesehatan, khususnya di bidang medis. Penelitian ini bertujuan untuk mengevaluasi karakterisasi kolagen dan potensi antioksidan S. horrens . Metode eksploratif deskriptif digunakan. Sampel S. horrens diekstraksi menggunakan NaOH, CH3COOH, dan air suling untuk mendapatkan kolagen. Total hasil kolagen setelah pengeringan beku adalah 39,3%. Analisis FTIR menunjukkan puncak kolagen tertinggi pada Amida A 3280,89, yang menunjukkan peregangan OH. Analisis asam amino menggunakan HPLC mengungkapkan L-Leusin sebagai asam amino esensial tertinggi (10,6%) dan L-Metionin sebagai yang terendah (0,1%). Di antara asam amino nonesensial, L-Asam Glutamat adalah yang tertinggi (25,7%), dan L-Sistin terendah (0,5%). Aktivitas antioksidan dinilai menggunakan metode DPPH, ABTS, dan FRAP, dengan nilai IC50 sebesar 91,12 ppm untuk DPPH dan 117,17 ppm untuk ABTS. Uji FRAP menunjukkan nilai ekuivalen Trolox sebesar 153,90 mmol/g. Uji antioksidan dengan metode DPPH dan ABTS menunjukan kekutan antioksidan sedang dan untuk FRAP dalam kategori yang kuat. }, issn = {2528-3111}, pages = {499--506} doi = {10.14710/jkt.v27i3.23892}, url = {https://ejournal2.undip.ac.id/index.php/jkt/article/view/23892} }
Refworks Citation Data :
Stichopus horrens, a marine organism known for its numerous health benefits, particularly in the medical field, was the focus of this study, which aimed to evaluate the collagen characterization and antioxidant potential of this species. An exploratory descriptive method was employed. The S. horrens samples were extracted using NaOH, CH3COOH, and distilled water to obtain collagen. After freeze-drying, the total collagen yield was 39.3%. FTIR analysis revealed the highest collagen peak at Amide A (3280.89 cm⁻¹), indicating O-H stretching. Amino acid analysis using HPLC showed L-Leucine as the highest essential amino acid (10.6%), while L-Methionine was the lowest (0.1%). Among non-essential amino acids, L-Glutamic acid was the highest (25.7%), with L-Cystine as the lowest (0.5%). Antioxidant activity was evaluated using DPPH, ABTS, and FRAP assays. The IC50 values were 91.12 ppm for DPPH and 117.17 ppm for ABTS. The FRAP assay indicated a Trolox equivalent value of 153.90 mmol/g. Antioxidant evaluation via DPPH and ABTS assays showed moderate antioxidant strength, while the FRAP assay indicated strong antioxidant potential.
Teripang Stichopus horrens, organisme laut yang memiliki berbagai manfaat kesehatan, khususnya di bidang medis. Penelitian ini bertujuan untuk mengevaluasi karakterisasi kolagen dan potensi antioksidan S. horrens. Metode eksploratif deskriptif digunakan. Sampel S. horrens diekstraksi menggunakan NaOH, CH3COOH, dan air suling untuk mendapatkan kolagen. Total hasil kolagen setelah pengeringan beku adalah 39,3%. Analisis FTIR menunjukkan puncak kolagen tertinggi pada Amida A 3280,89, yang menunjukkan peregangan OH. Analisis asam amino menggunakan HPLC mengungkapkan L-Leusin sebagai asam amino esensial tertinggi (10,6%) dan L-Metionin sebagai yang terendah (0,1%). Di antara asam amino nonesensial, L-Asam Glutamat adalah yang tertinggi (25,7%), dan L-Sistin terendah (0,5%). Aktivitas antioksidan dinilai menggunakan metode DPPH, ABTS, dan FRAP, dengan nilai IC50 sebesar 91,12 ppm untuk DPPH dan 117,17 ppm untuk ABTS. Uji FRAP menunjukkan nilai ekuivalen Trolox sebesar 153,90 mmol/g. Uji antioksidan dengan metode DPPH dan ABTS menunjukan kekutan antioksidan sedang dan untuk FRAP dalam kategori yang kuat.
Article Metrics:
Last update:
Upon acceptance for publication, authors agree to transfer the copyright of their article to Jurnal Kelautan Tropis, while retaining the right to reuse their work under the terms of the open license applied.
From the date of publication, the copyright for each article is held by Jurnal Kelautan Tropis. This transfer allows the journal to manage, disseminate, and preserve scholarly content in accordance with international standards and open access best practices.
Although copyright is held by the journal, all published articles are made available under the Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA 4.0). Under this license, anyone may:
Copy and redistribute the material in any medium or format
Remix, transform, and build upon the material for any purpose, even commercially
provided that:
Appropriate credit is given to the original author(s) and the source
Indications are made of any changes that were made
Derivative works are distributed under the same license (CC BY-SA 4.0)
While copyright is held by the journal, authors retain important reuse rights. Authors may:
Reuse the published version of their article in future works, including books, compilations, and lectures
Deposit the published version in institutional or subject repositories
Share the article freely, including on personal websites or academic networks
as long as the original publication in Jurnal Kelautan Tropis is cited and the CC BY-SA 4.0 license terms are respected.
Authors must ensure that any third-party content included in the article (e.g., figures, images, datasets) is either original, in the public domain, or licensed for reuse under compatible terms. If specific permissions are required, authors must obtain them prior to submission.
For questions regarding copyright or licensing, please contact the editorial office at: j.kelautantropis@gmail.com
View My Stats
Jurnal Kelautan Tropis is published by Departement of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro under a Creative Commons Attribution-ShareAlike 4.0 International License.
