skip to main content

Profil Fitokomponen dan Aktivitas Ekstrak Metanol Daun Intaran Bali (Azadiractha indica Juss.) terhadap Candida albicans ATCC-10231

Meylana Ayu Melinda orcid scopus  -  Universitas Udayana, Indonesia
*Agung Nova Mahendra orcid scopus  -  Universitas Udayana, Indonesia

Citation Format:
Abstract

Background: Candidiasis, usually caused by Candida albicans is commonly found in clinical settings. Resistance of C. albicans against antifungals is an emerging issue. This study was aimed to determine the selected quantitative phytocomponent profile and evaluate the effectiveness of intaran (Azadiractha indica A. Juss) leaf extract from Bali in inhibiting the growth of C. albicans .

Methods: This experimental research was conducted at the Center for Marine Aquaculture Research and Development (Buleleng, Bali), Laboratory Service Unit, Faculty of Agricultural Technology, Udayana University, and Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Udayana University, Denpasar, Bali. Our study included the preparation of the methanol extract of intaran leaves using maceration method, phytochemical analysis, and testing the antifungal activity of the extract using the diffusion disk assay against C. albicans ATCC-10231.

Results: Phytochemical components in the methanol extract of intaran Bali leaves were chlorophyll (18434.47 mg/L), total antioxidants (4818.07 mg/L GAEAC), phenol (4391.16 mg/100gr GAE) and flavonoids (785.32 mg/100gr QE). The extract exhibited dose-dependent effect, with greatest inhibition against C. albicans ATCC-10231 growth was obtained at the concentration of 50%.

Conclusion: Methanol extract of Balinese intaran leaves contained significant amount of chlorophyll, total antioxidants, phenol, and flavonoids. The extract showed antifungal activity against C. albicans ATCC-10231. This anticandidal effect is assumed to be related to the phytocomponents of the extract.

Fulltext View|Download
Keywords: Azadiractha indica A. juss; ekstrak daun; fitokomponen; aktivitas antijamur; Candida albicans

Article Metrics:

  1. Bongomin, F., Gago, S., Oladele, R., Denning, D. 2017 Global and Multi-National Prevalence of Fungal Diseases—Estimate Precision. J. Fungi. 3: 57
  2. Lu, M.; Li, T.;Wan, J.; Li, X.; Yuan, L.; Sun, S. 2017. Antifungal effects of phytocompounds on Candida species alone and in combination with fluconazole. Int. J. Antimicrobial. Agents. 49: 125–136
  3. Costa-de-Oliveira, S., Rodrigues, A.G. 2020. Candida albicans Antifungal Resistance and Tolerance in Bloodstream Infections: The Triad Yeast-Host-Antifungal. Microorganisms. 8 (154): 1-19
  4. Jin, YS. 2019. Recent advances in natural antifungal flavonoids and their derivatives. Bioorganic & Medicinal Chemistry Letters. 29 (19): 1-13
  5. Hamzah, H., Hertiani, T., Pratiwi, S.U.T., dan Nuryastuti, T., 2020. Inhibitory Activity and Degradation of Curcumin as Anti-Biofilm Polymicrobial on Catheters. International Journal of Research in Pharmaceutical Sciences. 11: 830–835
  6. Kumar, V.S., Navaratnam V. 2013. Neem (Azadirachta indica): Prehistory to contemporary uses to humankind. Asian Pac J Trop Biomed. 3 (7): 505-514
  7. Marangoni, A., Foschi, C., Micucci, M., Palomino, R.A.N., Toschi, T.G., Vitali, B. et al. 2017. In vitro activity of Spirulina platensis water extract against different Candida species isolated from vulvo-vaginal candidiasis cases. PLOS ONE. 12 (11): 1-17
  8. Huyut Z, Beydemir S, Gülçin E. 2017. Antioxidant and Antiradical Properties of Selected Flavonoids and Phenolic Compounds. Biochem Res. Int. 17: 1-10
  9. Sucindra Dewi, Agung Nova, Widi Kencana, Made Jawi, Made Sukrama, dan Ni Luh Kartini. 2017. Ethanolic extract of the powder of red earthworm (Lumbricus rubellus) obtained from several organic farmlands in Bali, Indonesia: Analysis of total phenolic content and antioxidant capacity. Bali Medical Journal. 3 (3): 80-83
  10. Salim, E., Afritunando, Y., Febriana, A., dan Mai Efdi. 2019. Studi Optimasi Ekstraksi Kandungan Senyawa Fenolik Total dan Uji Aktivitas Antioksidan dari Daun Manggis (Garcinia mangostana Linn.). Jurnal Riset Kimia. 10 (1): 36-43
  11. Truong, D.H., Nguyen, D.H., Ta, N.T.A., Bui, A.V., Do, T.H., Nguyen H.C. 2019. Evaluation of the Use of Different Solvents for Phytochemical Constituents, Antioxidants, and In vitro Anti-Inflammatory Activities of Severinia buxifolia. Journal of Food Quality.1-9
  12. Mohanad Jawad Kadhim, Ghaidaa Jihadi Mohammed and Imad Hadi Hameed. 2016. In vitro Antibacterial, Antifungal and Phytochemical Analysis of Metanolic Extract of Fruit Cassia fistula. Oriental Journal Of Chemistry. 32 (3): 1329-1346
  13. Al Aboody, M.S., Mickymarray, S. 2020. Anti-Fungal Efficacy and Mechanisms of Flavonoids. Antibiotics. 9 (45): 1-42
  14. Benisheikh AAG, Muhammad FM, Kelluri H, Aliyu ZM, Mallam UB, Jibrin MW. 2019. Phytochemical Extraction and Antimicrobial Studies on Crude Leaf Extract of Azadirachta indica (Neem) in Semi-Arid Region of Borno State, Nigeria. International Journal of Research and Review. 6 (12): 516-522

Last update: 2021-07-26 00:03:16

No citation recorded.

Last update: 2021-07-26 00:03:16

No citation recorded.