Antibacterial Activity from Temulawak Extract (Curcuma xanthorrhiza Roxb) on Growth Inhibition of Staphylococcus epidermidis In Vitro

*Ni Wayan Mutiara Warmasari  -  Universitas Udayana, Indonesia
Desak Ketut Ernawati  -  Universitas Udayana, Indonesia
Agung Wiwiek Indrayani  -  Universitas Udayana, Indonesia
Ni Wayan Sucindra Dewi  -  Universitas Udayana, Indonesia
I Made Jawi  -  Universitas Udayana, Indonesia
Received: 25 Dec 2019; Published: 27 Feb 2020.
Open Access License URL: http://creativecommons.org/licenses/by-sa/4.0

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Section: Articles
Language: EN
Statistics: 339 203
Abstract

Background: Infection is one of the main causes of disease in tropical regions like Indonesia. One of them can be caused by bacterial infection of Staphylococcus epidermidis. Staphylococcus epidermidis is an opportunistic bacterium that attacks individuals when the body's immune system is weak. The incidence of bacterial resistance to antibiotics that already exist so that requires an alternative treatment, one of which is the use of temulawak rhizome extract (Curcuma xanthorrhiza Roxb). Temulawak rhizome is one of the spices which is still often used as traditional medicine among Indonesian people. Temulawak rhizome extract is known to have a variety of chemical contents with active compounds in the form of essential oils xanthorrhizol and curcumin which can affect the rate of bacterial growth.

Methods: This study aims to determine the antibacterial activity of temulawak rhizome extract on the growth of Staphylococcus epidermidis bacteria in vitro. The method used in this study is the true experimental post test only control group design. Samples were divided into four treatment concentrations such as the extract concentration of 25%, 50%, 75%, and 100% of temulawak rhizome which was tested by agar diffusion method (Kirby-Bauer).

Result: The results of the analyze of variant (ANOVA) test showed a significant difference (p = 0,000). The temulawak extract could inhibit bacterial growth with a medium inhibitory response (5-10 mm). Temulawak rhizome extract showed the greatest inhibition at 100% concentration with an average inhibition zone diameter of 9.20 mm.

Conclusion : The extract of temulawak rhizome has antibacterial activity. The higher concentration of temulawak rhizome extract (Curcuma xanthorrhiza Roxb), the greater ability to inhibit the growth of Staphylococcus epidermidis bacteria.

 

Keywords: Antibacteria; Curcuma xanthorrhiza Roxb; Staphylococcus epidermidis; In Vitro; Growth Inhibition

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  1. Anjos LM, Marcondes MB, Lima MF, Mondelli AL, Okoshi MP. 2014. Streptococcal acute pharyngitis. Revista da Sociedade Brasileira de Medicina Tropical. 47 (4): 409-413.
  2. Movita T. 2013. Acne Vulgaris – Clinical Aspects. Continuing Medical Education Journal – 203. 40 (4): 269-272
  3. Pinheiro L, Brito CI, de Oliveira A, Martins PY, Pereira VC, da Cunha Mde L. 2015. Staphylococcus epidermidis and Staphylococcus haemolyticus: Molecular Detection of Cytotoxin and Enterotoxin Genes. Toxins. 7: 3688-3699.
  4. Lieve M, Shahrooei M, Hofmans D, Eldere JV. 2012. Immunoprophylaxis and immunotheraphy of Staphylococcus epidermidis infections: challenges and prospects. Expert Rev. Vaccines. 11 (3): 319-334
  5. Salea R, Edward W, Bambang Veriansyah, and Raymond R. 2014. Optimizing oil and xanthorrhizol extraction from Curcuma xanthorrhiza Roxb. Rhizome by supercritical carbon dioxide. J Food Sci Technol. 51 (9): 2197-2203
  6. Leite B, Gomes F, Teixera P, Pizzolitto E, Olivia CW, Oliveira R. 2011. In vitro Activity of Daptomycin, Linezolid and Rifampicin on Staphylococcus epidermidis Biofilms. Curr Microbiol. 63:313-317
  7. Mashita, A. 2017. Efek Antimikroba Ekstrak Rimpang Temulawak (Curcuma xanthorrhiza) Terhadap Pertumbuhan Staphylococcus Aureus. Saintika Medika. 10(2): 138.
  8. Mekni MA, Achour W, Ben HA. 2017. Overview of Genetic Background Beyond Polysaccharide Intercellular Adhesion Production in Staphylococcus epidermidis. Jundishapur J Microbiol. 10 (1): 2-12.
  9. Dermawaty, D. E. 2015. Potential Extract Curcuma ( Curcuma Xanthorrizal , Roxb ) As Antibacterial. Majority. 4 :5–11.
  10. Amalia, R. And Sari, R. 2017. Penentuan Nilai FICI Kombinasi Ekstrak Kulit Daun Lidah Buaya (Aloe Vera (L.) Burm. F.) Dan Gentamisin Sulfat Terhadap Bakteri Staphylococcus Aureus. Traditional Medicine Journal. 22 (3) : 175–181.
  11. Ngadino, Setiawan, Koerniasari, Ernawati, Sudjarwo SA. 2018. Evaluation of antimicrobacterial activity of Curcuma xanthorrhiza ethanolic extract against Mycoacterium tuberculosis H37Rv in vitro. Veterinary world. 11(3): 368-372
  12. Suhartati, R. 2018. Aktivitas Antibakteri Ekstrak Etanol Kulit Buah Naga Merah (Hylocereus Polyrhizus) Terhadap Bakteri Streptococcus Pyogenes. Jurnal Kesehatan Bakti Tunas Husada: Jurnal Ilmu-Ilmu Keperawatan. Analis Kesehatan Dan Farmasi. 17(2) : 513.
  13. Rekik A. 2012. Anti-inflammatory and Antimicrobial Effects of Heatclearing Chinese Herbs: A Current Review. Journal of Traditional and Complementary Medicine. 4(2): 93‑98
  14. Suswati, I. 2017. Efek Ekstrak Daun Sirih Merah (Piper Crocatum Ruiz & Pav) Terhadap Pertumbuhan (Streptococcus Pneumoniae). Saintika Medika. 8(1) : 1–5.
  15. Khaerana. 2008. Pengaruh Cekaman Kekeringan Dan Umur Panen Terhadap Pertumbuhan Dan Kandungan Xanthorrhizol Temulawak (Curcuma xanthorrhiza Roxb.). Pengaruh Cekaman Kekeringan Dan Umur Panen Terhadap Pertumbuhan Dan Kandungan Xanthorrhizol Temulawak (Curcuma xanthorrhiza Roxb.). 36(3) : 241–247.
  16. Purnamaningsih, N. 2017. Uji Aktivitas Antibakteri Ekstrak Temulawak (Curcuma xanthorrhiza) Terhadap Bakteri Escherichia Coli ATCC 11229 Dan Staphylococcus Aureus ATCC 25923. Jurnal Penelitian Saintek. 22(2) : 140–147.
  17. Jawetz, E., Melnick, J. L., Adelberg, E. A. (2008). Medical microbiology. Jakarta: Salemba Medika.
  18. Suhartati, R. 2018. Aktivitas Antibakteri Ekstrak Etanol Kulit Buah Naga Merah (Hylocereus Polyrhizus) Terhadap Bakteri Streptococcus Pyogenes. Jurnal Kesehatan Bakti Tunas Husada: Jurnal Ilmu-Ilmu Keperawatan. Analis Kesehatan Dan Farmasi. 17(2) : 513.