The Effect of a Mixture of Amylase and Cellulase Enzymes at Four Concentrations on Biogas Production from Rice Husk Waste under Liquid Anaerobic Digestion (L-AD) Conditions

Debora Christina  -  Universitas Diponegoro, Indonesia
*Syafrudin Syafrudin  -  Universitas Diponegoro, Indonesia
Winardi Dwi Nugraha  -  Universitas Diponegoro, Indonesia

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Abstract
Cellulose, hemicellulose, and lignin are some of the components found in rice husks, where cellulose plays an important role as a substrate for biogas fermentation. This study aims to determine the optimum concentration of amylase-cellulase enzyme mixture added to rice husks using the liquid anaerobic digestion (L-AD) method. Rice husks from the Rowosari area were chemically pretreated by soaking in 6% NaOH for 24 hours (control), then given biological pretreatment with varying enzyme concentrations of 9%, 12%, 15%, and 18%. The digestion process was carried out in batches using a 200 mL bioreactor, and the biogas volume was monitored every two days for 60 days. The total biogas yields at enzyme concentrations of 9%, 12%, 15%, and 18% were 5; 10; 11; and 13 mL/grTS, respectively, while the control reactor produced 9 mL/grTS. The highest production was achieved at an enzyme concentration of 18% with a maximum potential (A) of 13 mL/grTS, a production rate (U) of 1 mL/grTS·day, and a minimum biogas formation time (λ) of 1 day.
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Keywords: amylase enzyme; biogas production; cellulase enzyme; liquid anaerobic digestion; rice husk;
Article Info
Section: Original Research Paper
Language : EN
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