EKSPRESI GEN PENYANDI PEROKSIDASE CABAI MERAH (Capsicum annuum L.) (CaPer) SEBAGAI RESPONS TERHADAP Fusarium oxysporum

*Athried Elsima  -  Universitas Diponegoro, Indonesia
Hermin Pancasakti Kusumaningrum  -  Universitas Diponegoro, Indonesia
Rejeki Siti Ferniah  -  Universitas Diponegoro, Indonesia

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Abstract
Cabai merah (Capsicum annuum L.) merupakan komoditas pertanian penting di Indonesia yang sering terkena serangan OPT ( organisme pengganggu tanaman) misalnya Fusarium oxysporum. Tanaman cabai merah memiliki sifat ketahanan kimiawi aktif yaitu mengekspresikan peroksidase sebagai respons terhadap infeksi patogen. Penelitian ini bertujuan untuk mengetahui ekspresi gen penyandi peroksidase pada tanaman cabai merah sebagai respons terhadap jamur F. oxysporum. Penelitian ini diawali dengan inokulasi jamur pada tanaman cabai merah dilakukan dengan metode rendaman akar. Analisis ekspresi gen penyandi peroksidase menggunakan qRT-PCR dilakukan pada gen CaPer dengan gen 18s rRNA sebagai gen pembaku, pada interval 6, 48, dan 96 jam setelah inokulasi. Hasil penelitian gen CaPer terekspresi paling tinggi pada 6 jam setelah inokulasi.
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