Isolasi Khamir Fermentatif dari Batang Tanaman Tebu (Saccharum officinarum. L) dan Hasil Identifikasinya Berdasarkan Sekuens Internal Transcribed Spacer

*Ika Anggraini  -  Laboratorium Bioteknologi, Departemen Biologi, Fakultas Sains dan Matematika Universitas Diponegoro| Universitas Diponegoro, Indonesia
Rejeki Siti Ferniah  -  Laboratorium Bioteknologi, Departemen Biologi, Fakultas Sains dan Matematika Universitas Diponegoro| Universitas Diponegoro, Indonesia
Endang Kusdiyantini  -  Laboratorium Bioteknologi, Departemen Biologi, Fakultas Sains dan Matematika Universitas Diponegoro| Universitas Diponegoro, Indonesia

Citation Format:
Abstract

Yeast is a single-celled fungus that acts as epiphytes, endophytes or parasites. Yeast is divided into fermentative yeast and oxidative yeast. Fermentative yeast can produce primary and secondary metabolites. The role of fermentative yeast is widely used in the food industry, health and energy, so necessary to be explore fermentative yeast from sugarcane stems. The purpose of this study was to isolate fermentative yeast from sugarcane stems and identify molecular yeast based on the Internal Transcribed Spacer sequences (ITS). Isolation of epiphytic and endophytic yeast was carried out by spread plate of water soak sugarcane and sugar cane juice. Yeast isolation using 2 media, PDA and YGP with chloramphenicol. Morphological characterization was carried out by observing macroscopic and microscopic characteristics. Biochemical characterization was carried out by carbohydrate fermentation test and 50% glucose media growth test. Selected isolates were molecularly identified using Internal Transcribed Spacer (ITS) sequences. Primers used are ITS 1 and ITS 4. Phylogenetic analysis using Neighbor Joining from MEGA-6 program. The results of isolation obtained 7 yeast isolates characterized morphologically and biochemically. The based result of morphology and biochemical characterization were found 1 selected isolate with name Ed 1B. Selected yeast isolate have characteristics are round colonies, creamy white, shiny surface, raised elevation, wavy edges, ovoid cell shape, cell diameter 4,74µm, budding, glucose fermentation and sucrose fermentation, but not for lactose and grow well of 50% glucose media. The results of the Basic Alignment Search Tools (BLAST) are Ed 1B isolates had 99% homology with Kodamaea ohmeri species.

Fulltext View|Download
Article Info
Section: Articles
Language : ID
Recent articles
Identifikasi Chaetoceros sp. Secara Molekular dan Uji Antioksidan Karotenoid Antibacterial Activity Tests of Endophytic Bacteria Isolates From Tea Plant (Camellia sinensis) Againts Staphylococcus aureus and Staphylococcus epidermidis Biofilm Inhibition Activity from Obligate Marine Fungi Against Pathogenic Vibrio Bacteria in Whiteleg Shrimp, Penaeus vannamei More recent articles
  1. Afshan, N., Jan, M., Hamid, M., Nawaz, B., Jabeen, D, Ashraf, A., Alam, S. 2017 Isolation and Characterization of Indigenous Yeast Species from Yoghurt and Sugarcane Juice for Production of Bio-ethanol. PSM Microbiology 2(1): 9-
  3. Bhowmik, G. 2011. Ana Techniqs in Biotechnology. Tata McGraw Hill Education Private Limited, New Delhi : 92 – 93
  4. Boyd, A. R., Gunasekera, T. S., Attfield, P. V., Simic, K., Vincent, S. F., Veal, D. A. 2003. A Flow-Cytometric Method For Determination Of Yeast Viability And Cell Number In A Brewery. FEMS Yeast Research 3
  5. Bubnová, M., Zemančíková, J., And Sychrová,
  6. H. 2014. Osmotolerant Yeast Species
  8. Differ In Basic Physiological Parameters And In Tolerance Of Non-Osmotic Stresses. Yeast 31: 309–321
  9. Dharmayanti, N. L. P. I. 2011. Filogenetika Molekuler: Metode Taksonomi Organisme Berdasarkan Sejarah Evolusi. Wartazoa 21(1)
  10. Desjardins, P., Conklin, D. 2010. NanoDrop Microvolume Quantitation Of Nucleic Acids. Journal of Visualized Experiments and Thermo Fisher Scientific
  11. Federhen, S. 2015. Type material in the NCBI Taxonomy Database. Nucleic Acids Research
  12. Gana, N. H. T., Mendoza, B. C., And Monsalud, R. G. 2014. Isolation, Screening and Characterization of Yeasts with Amyloytic, Lipolytic, and Proteolytic Activities from the Surface of Philippine Bananas (Musa spp.). Philippine Journal of Science 143 (1): 81-
  14. Giri, S., Kindo, M. J. 2015. Evaluation Of Five Phenotypic Tests In The Identification Of Candida Species. National Journal Of Laboratory Medicine 4(4): 13-18
  15. Guo, C., Zhao, C., He, P., Lu, D., Shen, A and Jiang, N. 2006. Screening and characterization of yeasts for xylitol production. Journal of Applied Microbiology
  16. Haddad, R., Alemzadeh, E., Ahmadi, A. R., Hosseini, R., Moezzi, M. 2017. Identification of Chlorophyceae based on 18S rDNA sequences from Persian Gulf. Iran. J Microbiol 6(6): 437-442
  17. Hidayat, N., Wignyanto, Sumarsih, S., Putri,
  18. A. I. 2016. Mikologi Industri. UB Press, Surabaya
  19. Hikmatyar, M. F., Royani, J. I., Dasumiati. 2015. Isolasi Dan Amplifikasi DNA Keladi Tikus (Thyponium Flagelliform) Untuk Identifikasi Keragaman Genetik. J Bioteknol Bios Indon 2( 2): 42-48
  20. Intan, R.M.T., Cholil, A., Sulistyowati, L. 2014. Potensi Antagonis Jamur Endofit dan Khamir Pada Tanaman Pisang (Musa accumunata ) Terhadap Jamur Mycosphaerella musicola Penyebab Penyakit Bercak Kuning Sigatoka. Jurnal HPT 2(4)
  21. Isci, B., Yildirim, H. K., Altindisli, A. 2014. Evaluation of Methods for DNA
  24. Extraction from Must and Wine. J Inst Brew 120: 238–243
  25. Jimoh, S.O., Ado, S. A., Ameh, J. B., Whong,
  26. C. M. 2012. Osmotolerance and Fermentative Pattern of Brewer’s Yeast. World J Life Sci. and Medical Research 2(2): 59
  27. Khattab, S. M. R., Hadi, A. M. A., Dahab, N
  28. F. A., Atta, O. M. 2016. Isolation, Characterization, and Identification of Yeasts Associated with Foods from Assiut City, Egypt. BMRJ 13(1): 1-10
  29. Koffi, O., Samagaci, L., Goualie, B., Niamke,
  30. S. 2017. Diversity of Yeasts Involved in Cocoa Fermentation of Six Major Cocoa- Producing Regions in Ivory Coast. European Scientific Journal 13(30)
  31. Korabecna, M. 2003. The Variability in the Fungal Ribosomal DNA (ITS1, ITS2, and 5.8 S rRNA Gene): Its Biological Meaning and Application in Medical Mycology. Communicating Current Research and Educational Topics and Trends in Applied Microbiology
  32. Kurtzman, C.P., and Fell. 2011. The Yeast A Taxonomy Study. Biodiversity and Ecophysiology of Yeast. Springer-verlag, Berlin
  33. Lamey, P., Selemi, M., and Vandamme, A. M. 2009. The Phylogenetic Handbook: A Practical Approach to Phylogenetic Analysus and Hypothesis Testing. Cambrige University Press, UK
  34. Lasmini, T. 2016. Isolasi Dan Identifikasi Khamir Penghasil Asam Indol Asetat Dari Rhizosfer Anggrek Tanah Pecteilis Susannae (L.) Rafin. Jurnal Ipteks Terapan
  35. Masoud, W., Cesar, L. B., Jespersen, L., And Jakobsen, M. 2004. Yeast involved in fermentation of Coffea arabica in East Africa determined by genotyping and by direct denaturating gradient gel electrophoresis. Yeast 21: 549–556
  36. Mycobank. 2018. Kodamaea ohmeri. http://www.mycobank.org/BioloMICS
  37. Agustus 2018
  38. Obasi, B. C., Whong, C. M. Z., Ado, S. A., and Abdullahi, I. O. 2017. Leavening Ability Of Some Wild Yeasts And The Mutant Species Isolated From Fermented Orange Juice In Bakery Product (Bread). FUW Trends In Science & Technology Journal 2(1)
  40. Obasi, B.C., Whong, C. M. Z., Ado, S. A., and Abdullahi, I.O. 2014. Isolation and Identification Of Yeast Associated With Fermented Orange Juice. The International Journal Of Engineering And Science (IJES) 3 (9): 64-69
  41. Okwulehie, Cyriacus, I., Alfred, Kingsley, N. 2010. Fungi Associated With Deterioration Of Sour-Sop (Anona muricata. linn) Fruits In Abia State, Nigeria. African Journal Of Microbiology Research 4 (3): 143-146
  42. Periadnadi., Sari, D. K., Nurmiati. 2018. Isolaso dan Keberadaan Khamir Potensial Pemfermentasi Nira Aren (Arenga Pinnata Merr.) dari Dataran Rendah dan Dataran Tinggi di Sumatera Barat. Bioeksperimen 4(1) : 29-
  44. Purnamasari, M. I., Prihatna, C., Gunawan, A.W., dan Suwanto, A. 2012. Isolasi dan Identifikasi Secara Molekuler Ganoderma spp. yang Berasosiasi dengan Penyakit Busuk Pangkal Batang di Kelapa Sawit. Jurnal Fitopatologi Indonesia 8(1)
  45. Rada, A. H., dan Kasaie, Z. 2017. A Comparative Study On Different Methods For The Evaluation Of Baker’s Yeast Bioactivity. International Journal Of Food Properties 20(1): 100–106
  46. Rahmansyah, dan Kanti, A. 1999. Isolat-Isolat Khamir dari Minuman Tradisional Laru Di NTT. Berita Biologi 4(5)
  47. Rashid, A. N. M. M. O., Dash, B. K.,
  48. Chowdhury, N. A. 2013. Exploration of Potential Baker’s Yeast From Sugarcane Juice: Optimation and Evaluation. Pakistan Journal Of Biological Sciences. 16(13): 617-623
  49. Sandy, Y.A., Djauhari, S., Sektiono, A. W. 2015. Indentifikasi Molekuler Jamur Antagonis Trichoderma harzianum Diisolasi dari Tanah Pertanian di Malang Jawa Timur. Jurnal HPT 3(3)
  50. Santoso, Yahya, Suryaningtyas, N. H., Rahayu, K. S. 2015. Deteksi mikrofilaria Brugia malayi pada nyamuk Mansonia spp dengan pembedahan dan metode PCR di Kabupaten Tanjung Jabung Timur. Aspirator 7(1):29-35
  51. Sari, R.M.T., Saputro, T. B., Muhibuddin, A. 2016. Uji Potensi Fermentasi Etanol Yeast Tanah yang Diisolasi dari Metode Budidaya Sdn di Daerah Batu Jawa
  54. Timur. Jurnal Sains dan Seni ITS 5(2): 2337-3520
  55. Sharma, S., Arora, A., Sharma, P., Singh, S., Nain, L., And Paul, D. 2018. Notable Mixed Substrate Fermentation By Native Kodamaea ohmeri Strains Isolated From Lagenaria siceraria Flowers And Ethanol Production On Paddy Straw Hydrolysates. Chemistry Central Journal 12:8
  56. Stamps, J. A.,Yang, L. H., Morales, V. M., Mills, K. L. B. 2012. Drosophila Regulate Yeast Density and Increase Yeast Community Similarity in a Natural Substrate. Plos One 7 (7)
  57. Sumerta, I.N., Kanti, A. 2017. Keragaman Jenis Khamir Penghasil Etanol yang Diisolasi dari Makanan Fermentasi di Kepulauan Riau. Jurnal Biologi Indonesia 13(1): 61-69
  58. Takrama, J. F., Kumi, W. O., Otoo, G., Addo, K., and Camu, N. 2015 Optimization of Cocoa Pulp Juice Fermentation with Yeast Starter Cultures of Cocoa Heap Fermentations. Journal of Agricultural Science and Food Technology 1 (3): 22-
  60. Talaro, K. P., dan Barry, C. 2012. Foundations In Microbiology. MC Graw-Hill Comparies, New York
  61. Tam, H.M., Diep, C. N. 2014. Isolation, characterization and identification of endophytic bacteria in sugarcane
  63. (Saccharum spp. L.) cultivated on soils of the Dong Nai province, Southeast of Vietnam. American Journal of Life Sciences 2(6): 361-368
  64. Tesfaw, A., Assefa, F. 2014. Current Trends in Bioethanol Production by Saccharomyces cerevisiae: Substrate, Inhibitor Reduction, Growth Variables, Coculture, and Immobilization. Hindawi Publishing Corporation International Scholarly Research Notices
  65. Tikka, C., Osuru, H. P., Atluri, N., Raghavulu,
  66. P. C. V., Yellapu, N. K., Mannur, I. S.,
  67. Prasad, U. V., Aluru, S., Varma, N., And Bhaskar, M. 2013. Isolation and characterization of ethanol tolerant yeast strains. Bioinformation 9(8): 421-425
  68. Widiastutik, N., Alami, N. H. 2014. Isolasi dan Identifikasi Yeast dari Rhizosfer Rhizopora mucronataWonorejo. Jurnal Sains dan Seni Pomits 3(1): 2337-3520
  69. Yamada, Y.,,Suzuki, T., Matsuda, M., And Mikata, K. 1995. The Phylogeny of Yamadazyma ohmeri (Etchells Et Bell) Billon-Grand Based On The Partial Sequences of 18S And 26S Ribosomal RNAs: The Proposal of Kodamaea Gen. Nov. (Saccharomycetaceae). Biosci Biotech Biochem 59 (6): 1172-1174

Last update:

No citation recorded.

Last update:

No citation recorded.