Identifikasi Jenis Pigmen dan Uji Potensi Antioksidan Ekstrak Pigmen Bakteri Serratia marcescens Hasil Isolasi dari Sedimen Sumber Air Panas Gedong Songo

*Adhitya Naufal  -  Laboratorium Bioteknologi, Departemen Biologi, Fakultas Sains dan Matematika Universitas Diponegoro| Universitas Diponegoro, Indonesia
Endang Kusdiyantini  -  Laboratorium Bioteknologi, Departemen Biologi, Fakultas Sains dan Matematika Universitas Diponegoro| Universitas Diponegoro, Indonesia
Budi Raharjo  -  Laboratorium Bioteknologi, Departemen Biologi, Fakultas Sains dan Matematika Universitas Diponegoro| Universitas Diponegoro, Indonesia

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Abstract

Pigments can be produced by plants, animals, and microbes, including the bacteria Serratia marcescens. Pigments are believed to be used as antibacterial, anticancer, antibiotic and antioxidant. The quality of the pigment is greatly determined by its type. The aim of this study was to identify the type of pigment produced by S. marcescens resulting from the sediment of Gedong Songo hot spring, and testing its antioxidant activity. S. marcescens was grown on Nutrient Broth (NB) medium for 96 hours at room temperature and sampled once every 6 hours for growth measurement and pigment measurement. Cell dry weight was used to measure the growth of S. marcescens, while the measurement of pigment production was done using spectrophotometer with 535 nm wavelength for red pigment and identification of pigment with Thin Layer Chromatography and UV-Vis and testing of antioxidant activity using carotene bleaching method. The results showed the measurement of pigment production has optimal point at 48 hours at 1,319 mg/L. Identification of pigment type S. marcescens using TLC obtained value of  0,8 with spectrophotometer wavelength at 536 nm. The value of antioxidant activity Pigment S. marcescens obtained at 13%.

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  8. Daniel, M.M. 1986. Handbook of U.S. Colorant for Food, Drugs, and Cosmetics. (2nd).:
  9. Wiley-Interscience publications, United State of America
  10. Irdawati. 2010. Pengaruh Jumlah Starter dan Waktu Fermentasi Terhadap Pigmen yang Dihasilkan oleh Monascus
  12. purpureus pada Limbah Ubi Kayu (Manihot utilisima). Eksata. 1: 19-24
  13. Madigan, M. T., John, M.T., and David, A.S. 2012. Brock biology of microorganisms13th ed. Pearson Education, Inc., Amerika
  14. Mahmoud, S.T., Khalid, J.K.L., and Reem,
  15. W.Y. 2015. Enchancement of Prodigiosin Production by Serratia marcescens S23 Via Introducing Microbial Elicitor Cells Into Culture Medium. Irai Journal of Science. 50(3): 1938-1951
  16. Manzila, I., Priyatno, T.P., Herlis., R., Rusmana, I., Samudra, I.M., dan Suryadi, Y. 2014. Pengaruh Media terhadap Produksi Prodigiosin Isolat Bakteri Entomopatogen Serratia marcescens Asal Wereng Batang Cokelat. Jurnal AgroBiogen. 10(2):77-84
  17. Phatake, Y.B., and Dharmadhikari, S.M. 2015. Studies On Production of Prodigiosin from Serratia marcescens and Statistical Optimization of Production Process by Using PBD. IJPRBS. 4(5): 146-159
  18. Pore, T.S., Ashwini, B.K. and Naiem, H.N. 2016. Production, Purification, Identification of Prodigiosin From Serratia sp. and Its Antimicrobial Activity. RJLBPCS. ISSN: 2454-6348
  19. Rosang, C. dan Billy T.W. 2016. Penentuan Kandungan Pigmen Klorofil pada Lamun Jenis Halophila ovalis di Perairan Malalayang. Jurnal Pesisir dan Laut Tropis. 1(1): 15-19
  20. Rowe, R.C., Sheskey, P.J., and Quinn, M.E. 2009. Handbook of Pharmaceutical Excipients. Pharmaceutical Press, Washington, DC
  21. Salamah, N., dan Nurushoimah. 2014. Uji aktivitas Antioksidan Ekstrak Etanol Herba Pegagagan (Centella asiatisca
  22. (L) Urb.) dengan Metode Penghambatan Degradasi Beta- Karoten. Farmasains. 2(4): 177-181
  23. Sarastani, Dewi, Soewarno T., Soekarto, T.R. Muchtadi, D.F., dan Anton A. 2002. Aktivitas Antioksidan Ekstrak dan Fraksinasi Ekstrak Biji Atung (Parinarium glaberrimum Hassk)
  25. Jurnal. Teknol. dan Industri Pangan
  26. (2): 149-156
  27. Shahitha, S., and Poornima, K. 2012. Enhanced Production of Prodigisin Productin in Serratia Marcescens. Jurnal of Applied Pharmaceutical Science. 2(8): 138-140
  28. Slonczewski, J. and Foster, J. 2009
  29. Microbiology: An Evolving Science
  30. W.W. Norton & Company, Inc., New York
  31. Song, M.J.; Bae, J.; Lee, D.S.; Kim, C.H.; Kim, J.S.; Kim, S.W.; and Hong, S.I. 2006
  32. Purification and Characterization of Prodigiosin Produced by Integrated Bioreactor from Serratia sp. KH-95. J. Biosci. Bioeng. 101: 157-161
  33. Thomas, H.W. 1985. Bailey’s Industrial Oil and Fat Product, Volume 3: Jhon Wiley & Son, New York
  34. Utami, T.S., Rita, A., Heri, H., dan Ahmad, R. 2009. Perbandingan Aktivitas Antioksidan Ekstrak Etanol Daun Simpur (Dillenia indica) dari Berbagai Metode Ekstraksi dengan Uji ANOVA. Fakultas Teknik Universitas Indonesia. Seminar Nasional Teknik Kimia Universitas Indonesia. ISBN: 987-99- 98300-1-2
  36. Venil, C.K. and Lakshmanaperumalsamy, P. 2009. Application of Statistical Design to The Optimization of Culture Medium for Prodigiosin Production by Serratia marcescens. Malaysian Journal of Microbiology. 5(1): 55-61
  37. Vora, J.U., Jain, N.K., and Modi, H.A. 2014. Extraction Characteruzation and Application Studies of Red Pigment of Halophile Serratia marcescens KH1R KM035849 Isolated from Kharaghoda Soil. Int J. Pure App. Biosct. 2(6): 160- 168
  38. Vora, J.U., Jain, N.K., and Modi, H.A. 2014. Extraction Characteruzation and Application Studies of Red Pigment of Halophile Serratia marcescens KH1R KM035849 Isolated from Kharaghoda Soil. Int J. Pure App. Biosct. 2(6): 160- 168
  39. Yuliana, N. 2008. Kinetika Pertumbuhan Bakteri Asam Laktat Islat T5 yang Berasal dari Tempoyak. Jurnal Teknologi Industri dan Hasil Pertanian. 13(2): 108-116

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