Dampak Inklusi In-ovo Feeding Vitamin E dan Selenium pada Histomorfometri Bursa Embrio Itik Hibrida

Abstract

Vitamin E and selenium are two important nutrients that play a role in maintaining body health and supporting vital organ function. The role of vitamin E (VE) and selenium (Se) as antioxidants can be utilized to improve duck hatchability. This study aims to analyze the histological structure of the bursa fabricius of duck embryos after in-ovo inclusion of vitamin E and selenium. This study used a completely randomized design (CRD) consisting of four treatments, namely K0 (control), K1 (in-ovo inclusion of 0.1 mL of 0.89% NaCl), K2 (in-ovo inclusion of 0.1 mL of VE and Se), and K3 (in-ovo inclusion of 0.15 mL of VE and Se). The treatments were administered when the eggs were 14 days old; egg incubation ended on day 26. Bursa preparations were made using the paraffin method and stained with hematoxylin-eosin. The parameters observed were the number and diameter of plicae, the number and diameter of follicles, the thickness of the epithelium, the diameter of the lumen, and the thickness of the cortex and medulla. The data were analyzed using Analysis of Variance (ANOVA) at a significance level of 5% (α = 0.05) and the Kruskal-Wallis test. The results showed a significant difference (P<0.05) in the number and diameter of follicles. The number and diameter of plicae,  epithelium thickness, lumen diameter, cortex and medulla thickness showed no significant differences (P>0.05). Based on the results of this study, it can be concluded that in-ovo injection of VE and Se has the potential to optimize the histological structure of the bursa fabricius follicles, which in turn supports the immune system of hybrid ducks.