DOI: https://doi.org/10.14710/aesj.v2i2.19471

Fitoremediasi Lahan Tercemar Pb menggunakan Helianthus annuus L. dengan Bacillus subtilis dan Pseudomonas aeruginosa

*C. N. Al-Hayah  -  Agroecotechnology, Universitas Diponegoro, Indonesia
S. Budiyanto  -  Agroecotechnology, Universitas Diponegoro, Indonesia
E. Fuskhah  -  Agroecotechnology, Universitas Diponegoro, Indonesia

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Abstract

This study aimed to evaluate the effect of adding various doses and types of bacteria on the growth and ability of sunflower plants to absorb Pb from polluted land. The research was carried out in the study used a factorial experiment with a completely randomized design (CRD). The first factor is the dose of bacteria consisting of (A) 25 ml/plant, 50 ml/plant, and 75 ml/plant. The second factor was the type of bacteria (B), which consisted of Bacillus subtilis, Pseudomonas aeruginosa, and Bacillus subtilis and Pseudomonas aeruginosa. The observation parameters included plant height, number of leaves, root length, dry matter, Pb levels in the soil, and Pb levels in the leaves, stems, and roots. The data obtained were processed using Analysis of Variance, followed by the Least Significant Difference Test at the 5% level. There is an interaction between the dose of bacteria and the type of bacteria on the parameters of the number of leaves, and root length. The dose of bacteria affects root length, Pb levels in leaves, and Pb levels in stems. The best bacterial dose was 75 ml. The treatment of bacterial species did not affect plant growth, Pb uptake in plants, and Pb adsorption in soil

 

Keywords: Bacillus subtilis, Pseudomonas aeruginosa, Pb

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Funding: niversitas Diponegoro under contract MetMu123456

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