DOI: https://doi.org/10.14710/sat.v10i2.33412

Molecular Identification and Antibacterial Activity of Mangrove Sediment Associated Fungi Against Vibrio harveyi

1Postgraduate program on Aquatic Resource Management, Faculty of Fisheries and Marine Science, , Indonesia

2Diponegoro University, Jl. Prof. H. Soedarto, S.H, Tembalang, Semarang, Jawa Tengah, Indonesia

3Department of Aquatic Resources, Faculty of Fisheries and Marine Science, Universitas , Indonesia

4 Diponegoro, Semarang, Indonesia , Indonesia

5 Master Program of Aquatic Resources Management, Faculty of Fisheries and Marine , Indonesia

6 Science, Universitas Diponegoro, Semarang, Indonesia , Indonesia

7 Tropical Marine Biotechnology Laboratory, Faculty of Fisheries and Marine Science, , Indonesia

8 University Diponegoro, Semarang, Indonesia, Indonesia

9 Science, Universitas Diponegoro, Semarang, Indonesia , Indonesia

10 Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas , Indonesia

11 Diponegoro, Semarang, Indonesia, Indonesia

12 Laboratory of Genome Science, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan, Japan

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Open Access Copyright 2026 Amelia Rahmawati, Aninditia Sabdaningsih, Sarjito Sarjito, Dion Saputra

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Abstract

Vibrio harveyi is one of the major bacterial pathogens causing vibriosis in shrimp aquaculture, leading to significant reductions in production and economic losses. This study aimed to molecularly identify a mangrove sediment-associated fungal isolate and evaluate its antibacterial activity against V. harveyi. The research was conducted experimentally through molecular identification using ITS1 and ITS4 primers, phylogenetic analysis, and antibacterial activity assessment using the agar plug method. The fungal isolate exhibiting the largest inhibition zone against V. harveyi was selected for further analysis. Molecular identification based on BLAST analysis revealed that isolate MS.02 shared 100% sequence similarity with Trichoderma yunnanense. Phylogenetic analysis further confirmed the close evolutionary relationship between isolate MS.02 and Trichoderma kunmingese.

Antibacterial testing showed that isolate MS.02 inhibited the growth of V. harveyi with a moderate inhibition category, producing a clear inhibition zone of 8.45 ± 3.65 mm around the test medium. The formation of the inhibition zone indicated the production of bioactive compounds with antibacterial properties. These findings highlight the potential of mangrove sediment-associated fungi as a natural source of antibacterial agents against V. harveyi. The study provides scientific evidence supporting the utilization of mangrove-derived fungi as environmentally friendly alternatives for disease management in shrimp aquaculture. In conclusion, isolate MS.02 possesses promising antibacterial activity against V. harveyi and may serve as a potential biocontrol agent for sustainable aquaculture practices.

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Funding: Master Thesis Research Scheme (BIMA), Ministry of Higher Education, Science and Technology, Republic of Indonesia under contract 601-118/UN7.D2/PP/VI/2024

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