DOI: https://doi.org/10.14710/sat.v10i2.33328

Dynamics of Dissolved Oxygen Release from Hydrogen Peroxide Under Different Temperature Regimes in Closed System

1Department of Aquatic Resource Management, Faculty of Fisheries and Marine Science, Diponegoro University, Jl. Prof. H. Soedarto, S.H, Tembalang, Semarang, Jawa Tengah, Indonesia

2Postgraduate program on Aquatic Resource Management, Faculty of Fisheries and Marine Science, Diponegoro University, Jl. Prof. H. Soedarto, S.H, Tembalang, Semarang, Jawa Tengah, Indonesia

3Aquaculture Departement, Faculty of Fisheries and Marine Science, Diponegoro University, Jl. Prof. H. Soedarto, S.H, Tembalang, Semarang, Jawa Tengah, Indonesia

4 Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium., Belgium

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Open Access Copyright 2026 Viola Nur Maghribfa, Slamet Budi Prayitno, Desrina Desrina, Sri Hastuti, Kanya Wijaya, Alfabetian Harjuno Condro Haditomo

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Abstract

Dissolved oxygen (DO) is an important parameter in aquaculture systems as it directly influences water quality stability and the biological activity of aquatic organisms. This study aimed to evaluate the dynamics of dissolved oxygen resulting from hydrogen peroxide decomposition under different temperature conditions in a closed system. The experiment was conducted at two temperature regimes, namely 25°C and room temperature (27–28°C), using hydrogen peroxide doses of 0 mL/L, 0.025 mL/L, 0.050 mL/L, and 0.075 mL/L. Observations were carried out over a 24-hour period, with measurements recorded every two hours. The results showed that the addition of hydrogen peroxide increased dissolved oxygen levels during the first four hours, after which the values tended to stabilize. At room temperature, dissolved oxygen fluctuations were higher compared to those at 25°C. The presence of fish in the water medium resulted in a more rapid increase in dissolved oxygen, starting from the first hour of observation. This indicates that biological activity accelerates hydrogen peroxide decomposition. However, dissolved oxygen gradually decreased over time in treatments with fish due to oxygen consumption by organisms and associated microbial activity. Water quality parameters indicated that temperature remained relatively stable throughout the observation period, whereas pH tended to decrease over time, particularly in treatments with fish. This decline is likely due to carbon dioxide accumulation resulting from respiration. Overall, the findings demonstrate that temperature and biological activity are the main factors influencing dissolved oxygen dynamics and the rate of oxygen release from hydrogen peroxide in a closed system.

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Funding: The authors gratefully acknowledge Mr. Alfabetian Harjuno Condro Haditomo and Slamet Budi Prayitno for their support and provision of research facilities.

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