The Microwave-assisted Synthesis of Polyethersulfone (PES) as A Matrix in Immobilization of Candida antarctica Lipase B (Cal-B)

*Khusna Widhyahrini -  Magister and Doctor Study Program, Department of Chemistry, Institut Teknologi Bandung, Bandung,, Indonesia
Nurrahmi Handayani -  Analitical Chemistry Division, Department of Chemistry, Institut Teknologi Bandung, Bandung,, Indonesia
Deana Wahyuningrum -  Organic Chemistry Division, Department of Chemistry, Institut Teknologi Bandung, Bandung, Indonesia
Santi Nurbaiti -  Biochemistry Division, Departement of Chemistry, Institut Teknologi Bandung, Bandung,, Indonesia
Cynthia Linaya Radiman -  Physical Chemistry Division, Department of Chemistry, Institut Teknologi Bandung, Bandung,, Indonesia
Received: 15 Nov 2016; Published: 28 Oct 2017.
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Section: The 2nd International Seminar on Chemistry (ISoC 2016) (Surabaya, 26-27 July 2016)
Language: EN
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In 2017: BCREC Volume 12 Issue 3 Year 2017 (SCOPUS and Web of Science Indexed, December 2017)
Statistics: 620 362

Abstract

Candida antarctica lipase B (Cal-B) has been widely used in the hydrolysis reaction. However, it has some weaknesses, such as: forming of the heavy emulsion during the process, which is difficult to resolve and has no reusability. Therefore, it needs to be immobilized into a suitable matrix. One of the suitable supporting materials is polyethersulfone (PES) and its synthesis becames the objective of this paper. The PES was synthesized via a polycondensation reaction between hydroquinone and 4,4'-dichlorodiphenylsulfonein N-methylpyrrolidone (NMP) as solvent using Microwave Assisted Organic Synthesis (MAOS) method at170 °C for 66 minutes using an irradiation power of 300 watt. The synthesized PES was characterized by FTIR and 1H-NMR (500 MHz, DMSO-d6). Then the PES membrane was prepared from 20 % of the optimized mixtures of PES, PSf (polysulfone), and PEG (polyethylene glycol) dissolved in 80 % NMP.  The Cal-B was immobilized on the PES membrane by mixing it in a shaker at 30 °C and 100 rpm for 24 h using phosphate buffered saline (PBS). The identification of the immobilized Cal-B was done by using FTIR-ATR spectroscopy and SEM micrographs. The results of Lowry assay showed that the ‘Cal-B immobilized’ blended-membrane has a loading capacity of 91 mg/cm2 in a membrane surface area of 17.34 cm2. In this work, the activity of immobilized Cal-B was twice higher than the native enzyme in p-NP (p-Nitrophenolpalmitate) hydrolyzing. The results indicated that the synthesized PES showed a good performance when used as a matrix in the immobilization of Cal-B. Copyright © 2017 BCREC Group. All rights reserved

Received: 15th November 2016; Revised: 27th May 2017; Accepted: 24th May 2017; Available online: 23rd October 2017; Published regularly: December 2017

How to Cite: Widhyahrini, K., Handayani, N., Wahyuningrum, D., Nurbaiti, S., Radiman, C.L. (2017). The Microwave-assisted Synthesis of Polyethersulfone (PES) as A Matrix in Immobilization of Candida antarctica Lipase B (Cal-B). Bulletin of Chemical Reaction Engineering & Catalysis, 12(3): 343-350 (doi:10.9767/bcrec.12.3.774.343-350)

 

Keywords
Polyethersulfone (PES); Microwave Assisted Organic Synthesis (MAOS); Immobilization; Lipase; Candida antarctica lipase B (Cal-B)

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