DOI: https://doi.org/10.9767/bcrec.12.3.774.343-350

The Microwave-assisted Synthesis of Polyethersulfone (PES) as A Matrix in Immobilization of Candida antarctica Lipase B (Cal-B)

1Magister and Doctor Study Program, Department of Chemistry, Institut Teknologi Bandung, Bandung, Indonesia

2Analytical Chemistry Division, Department of Chemistry, Institut Teknologi Bandung, Bandung, Indonesia

3Organic Chemistry Division, Department of Chemistry, Institut Teknologi Bandung, Bandung, Indonesia

4 Biochemistry Division, Departement of Chemistry, Institut Teknologi Bandung, Bandung, Indonesia

5 Physical Chemistry Division, Department of Chemistry, Institut Teknologi Bandung, Bandung, Indonesia

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Received: 15 Nov 2016; Revised: 23 May 2017; Accepted: 24 May 2017; Available online: 27 Oct 2017; Published: 1 Dec 2017.
Editor(s): Istadi Istadi, Yuly Kusumawati
Open Access Copyright (c) 2017 by Authors, Published by BCREC Group under http://creativecommons.org/licenses/by-sa/4.0.

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Abstract

Candida antarctica lipase B (Cal-B) has been widely used in the hydrolysis reaction. However, it has some weaknesses, such as: forming of the heavy emulsion during the process, which is difficult to resolve and has no reusability. Therefore, it needs to be immobilized into a suitable matrix. One of the suitable supporting materials is polyethersulfone (PES) and its synthesis becames the objective of this paper. The PES was synthesized via a polycondensation reaction between hydroquinone and 4,4'-dichlorodiphenylsulfonein N-methylpyrrolidone (NMP) as solvent using Microwave Assisted Organic Synthesis (MAOS) method at170 °C for 66 minutes using an irradiation power of 300 watt. The synthesized PES was characterized by FTIR and 1H-NMR (500 MHz, DMSO-d6). Then the PES membrane was prepared from 20 % of the optimized mixtures of PES, PSf (polysulfone), and PEG (polyethylene glycol) dissolved in 80 % NMP.  The Cal-B was immobilized on the PES membrane by mixing it in a shaker at 30 °C and 100 rpm for 24 h using phosphate buffered saline (PBS). The identification of the immobilized Cal-B was done by using FTIR-ATR spectroscopy and SEM micrographs. The results of Lowry assay showed that the ‘Cal-B immobilized’ blended-membrane has a loading capacity of 91 mg/cm2 in a membrane surface area of 17.34 cm2. In this work, the activity of immobilized Cal-B was twice higher than the native enzyme in p-NP (p-Nitrophenolpalmitate) hydrolyzing. The results indicated that the synthesized PES showed a good performance when used as a matrix in the immobilization of Cal-B. 

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Keywords: Polyethersulfone (PES); Microwave Assisted Organic Synthesis (MAOS); Immobilization; Lipase; Candida antarctica lipase B (Cal-B)

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Section: The 2nd International Seminar on Chemistry (ISoC 2016) (Surabaya, 26-27 July 2016)
Language : EN
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