Immobilised Chlorella vulgaris as An Alternative for The Enhancement of Microalgae Oil and Biodiesel Production

Nur Hanani Rushan  -  Faculty of Chemical & Process Engineering Technology, College of Engineering Technology, Universiti Malaysia Pahang, Malaysia
*Nur Hidayah Mat Yasin  -  Faculty of Chemical & Process Engineering Technology, College of Engineering Technology, Universiti Malaysia Pahang, Malaysia
Farhan Mohd Said  -  Faculty of Chemical & Process Engineering Technology, College of Engineering Technology, Universiti Malaysia Pahang, Malaysia
Nagaarasan Ramesh  -  Faculty of Chemical & Process Engineering Technology, College of Engineering Technology, Universiti Malaysia Pahang, Malaysia
Received: 25 Dec 2019; Revised: 4 Apr 2020; Accepted: 6 Apr 2020; Published: 1 Aug 2020; Available online: 30 Jul 2020.
Open Access Copyright (c) 2020 Bulletin of Chemical Reaction Engineering & Catalysis
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Microalgae are a promising alternative for biodiesel production and a valuable source of fatty acid methyl ester (FAME). In this research, Chlorella vulgaris has been chosen as the suitable microalgae because this species was able to produce highest oils for biodiesel processing. Previously, sodium alginate (SA) was used to entrap the microalgae in the culturing process due to its low toxicity and high transparency. However, SA have some disadvantages such as bead disruption which leading to the loss of microalgae cell. Therefore, this research has been conducted to evaluate the oil production of immobilised Chlorella vulgaris using different matric systems at different ratios which are 0.3:1, 1:1 and 2:1. Currently, six matric systems have been developed, they are SA as a control, a combination of SA and chitosan (SA+CT), SA and carrageenan (SA+CR), SA and gelatin (SA+GT), SA and calcium alginate (SA+CA), and SA and sodium carboxymethylcellulose (SA+CMC). The microalgae was first cultivated, harvested and extracted to produce oil, prior to use in the transesterification process. The SA+GT showed the highest oil yield with 59.14% and a total FAME of 0.56 mg/g. The FAME profile of oil extracted microalgae showed high potential for biodiesel production as it consisted of palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acid (C18:3). The results proved that the combination of SA+GT had improved the oil yield and fatty acid composition as compared to the other matric systems, which may have useful application for the biodiesel industry. Copyright © 2020 BCREC Group. All rights reserved


Keywords: Chlorella vulgaris; Immobilised; Oil yield; Matric systems; Biodiesel; Microalgae

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  1. Rajak, U., Nashine, P., Verma, T.N., Pugazhendhi, A. (2019). Alternating the environmental benefits of Aegle-diesel blends used in compression ignition. Fuel, 256, 115835. DOI: 10.1016/j.fuel.2019.115835
  2. Rajak, U., Nashine, P., Verma, T.N., Pugazhendhi, A. (2019). Performance, combustion and emission analysis of microalgae Spirulina in a common rail direct injection diesel engine. Fuel, 255, 115855. DOI: 10.1016/j.fuel.2019.115855
  3. Anto, S., Pugazhendhi, A., Mathimani, T. (2019). Lipid enhancement through nutrient starvation in Chlorella sp. and its fatty acid profiling for appropriate bioenergy feedstock. Biocatalysis and Agricultural Biotechnology, 20, 101179. DOI: 10.1016/j.bcab.2019.101179
  4. Chi, N.T.L., Duc, P.A., Mathimani, T., Pugazhendhi, A. (2019). Evaluating the potential of green alga Chlorella sp. for high biomass and lipid production in biodiesel viewpoint. Biocatalysis and agricultural biotechnology, 17, 184-188. DOI: 10.1016/j.bcab.2018.11.011
  5. Anto, S., Mukherjee, S.S., Muthappa, R., Mathimani, T., Deviram, G., Kumar, S.S., Pugazhendhi, A. (2020). Algae as green energy reserve: Technological outlook on biofuel production. Chemosphere, 242, 125079. DOI: 10.1016/j.chemosphere.2019.125079
  6. Mathimani, T., Pugazhendhi, A. (2019). Utilization of algae for biofuel, bio-products and bio-remediation. Biocatalysis and agricultural biotechnology, 17, 326-330. DOI: 10.1016/j.bcab.2018.12.007
  7. Saravanan, A.P., Mathimani, T., Deviram, G., Rajendran, K., Pugazhendhi, A. (2018). Biofuel policy in India: a review of policy barriers in sustainable marketing of biofuel. Journal of cleaner production, 193, 734-747. DOI: 10.1016/j.jclepro.2018.05.033
  8. Sivasankar, P., Poongodi, S., Lobo, A.O., Pugazhendhi, A. (2020). Characterization of a novel polymeric bioflocculant from marine actinobacterium Streptomyces sp. and its application in recovery of microalgae. International Biodeterioration & Biodegradation, 148, 104883. DOI: 10.1016/j.ibiod.2020.104883
  9. Lam, M.K., Yusoff, M.I., Uemura, Y., Lim, J.W., Khoo, C.G., Lee, K.T., Ong, H.C. (2017). Cultivation of Chlorella vulgaris using nutrients source from domestic wastewater for biodiesel production: Growth condition and kinetic studies. Renewable Energy, 103, 197-207. DOI: 10.1016/j.renene.2016.11.032
  10. Khan, M.I., Shin, J.H., Kim, J.D. (2018). The promising future of microalgae: current status, challenges, and optimization of a sustainable and renewable industry for biofuels, feed, and other products. Microbial cell factories, 17(1), 36. DOI: 10.1186/s12934-018-0879-x
  11. Sharma, J., Kumar, S.S., Bishnoi, N.R., Pugazhendhi, A. (2019). Screening and enrichment of high lipid producing microalgal consortia. Journal of Photochemistry and Photobiology B: Biology, 192, 8-12. DOI: 10.1016/j.jphotobiol.2019.01.002
  12. Sharma, J., Kumar, S.S., Bishnoi, N.R., Pugazhendhi, A. (2018). Enhancement of lipid production from algal biomass through various growth parameters. Journal of Molecular Liquids, 269, 712-720. DOI: 10.1016/j.molliq.2018.08.103
  13. Garlapati, D., Chandrasekaran, M., Devanesan, A., Mathimani, T., Pugazhendhi, A. (2019). Role of cyanobacteria in agricultural and industrial sectors: an outlook on economically important byproducts. Applied microbiology and biotechnology, 103(12), 4709-4721. DOI: 10.1007/s00253-019-09811-1
  14. Deviram, G., Mathimani, T., Anto, S., Ahamed, T.S., Ananth, D.A., Pugazhendhi, A. (2019). Applications of microalgal and cyanobacterial biomass on a way to safe, cleaner and a sustainable environment. Journal of Cleaner Production, 253, 119770. DOI: 10.1016/j.jclepro.2019.119770
  15. Rincon, S.M., Romero, H.M., Aframehr, W.M., Beyenal, H. (2017). Biomass production in Chlorella vulgaris biofilm cultivated under mixotrophic growth conditions. Algal Research, 26, 153-160. DOI: 10.1016/j.algal.2017.07.014
  16. Tandon, P., Jin, Q., Huang, L. (2017). A promising approach to enhance microalgae productivity by exogenous supply of vitamins. Microb. Cell Fact., 16, 219. DOI: 10.1186/s12934-017-0834-2
  17. Panahi, Y., Khosroushahi, A.Y., Sahebkar, A., Heidari, H.R. (2019). Impact of Cultivation Condition and Media Content on Chlorella vulgaris Composition. Advanced Pharmaceutical Bulletin, 9(2), 182–194. DOI: 10.15171/apb.2019.022
  18. Hamedi, S., Mahdavi, M.A., Gheshlaghi, R. (2016). Improved lipid and biomass productivities in Chlorella vulgaris by differing the inoculation medium from the production medium. Biofuel Res J, 3, 410–416. DOI: 10.18331/brj2016.3.2.6
  19. Mushtaq, F., Maqbool, W., Mat, R., Ani, F.N. (2013). Fossil fuel energy scenario in Malaysia-prospect of indigenous renewable biomass and coal resources. IEEE Conference on Clean Energy and Technology (CEAT). DOI: 10.1109/ceat.2013.6775632
  20. Difusa, A., Mohanty, K., Goud, V.V. (2015). Advancement and Challenges in Harvesting Techniques for Recovery of Microalgae Biomass. In Environmental Sustainability, 159-169.
  21. Lam, M.K., Lee, K.T. (2012). Immobilization as a feasible method to simplify the separation of microalgae from water for biodiesel production. Chem. Eng. J., 191, 263–268. DOI: 10.1016/j.cej.2012.03.013
  22. Eroglu, E., Smith, S.M., Raston, C.L. (2015). Application of various immobilization techniques for algal bioprocesses, in Biomass and Biofuels from Microalgae, in: Moheimani, N.R., McHenry, M.P., de Boer, K., Bahri, P. (Eds.). Biofuel and Biorefinery Technologies, 19–44. DOI: 10.1007/978-3-319-16640-7
  23. Vasilieva, S., Shibzukhova, K., Morozov, A., Solovchenko, A., Bessonov, I., Kopitsyna, M., Lobakova, E. (2018). Immobilization of microalgae on the surface of new cross-linked polyethylenimine-based sorbents. Journal of Biotechnology, 281, 31–38. DOI: 10.1016/j.jbiotec.2018.03.011
  24. Smidsrod, O., Skjakbrk, G. (1990). Alginate as immobilization matrix for cells. Trends in Biotechnology, 8, 71-78. DOI: 10.1016/0167-7799(90)90139-o
  25. Abu Sepian, N.R., Mat Yasin, N.H., Zainol, N., Rushan, N.H., Ahmad, A.L. (2019). Fatty acid profile from immobilised Chlorella vulgaris cells in different matrices. Environmental Technology, 40(9), 1110–1117. DOI: 10.1080/09593330.2017.1408691
  26. Culture Collection of Algae and Protozoa. (1 September 2017). Internet sources URL
  27. Abdullah, N., Amran, N.M., Mat Yasin, N.H. (2017). Algae Oil Extraction From Freshwater Microalgae Chlorella vulgaris. Malaysian Journal of Analytical Science, 21(3), 735-744. DOI: 10.17576/mjas-2017-2103-23
  28. Nawaz, K., Shelly, J., Jacobi, A.M. (2015). A parametric study on mass diffusion coefficient of desiccants for dehumidification applications: Silica aerogels and silica aerogel coatings on metal foams. Science and technology for the Built Environment, 21(5), 637-647, DOI: 10.1080/23744731.2015.1007835
  29. Gautier, A., Carpentier, B., Dufresne, M., Dinh, Q.V., Paullier, P., Legallais, C. (2011). Impact of alginate type and bead diameter on mass transfers and the metabolic activities of encapsulated C3A cells in bioartificial liver applications. European Cells and Materials, 21, 94–106. DOI: 10.22203/ecm.v021a08
  30. Alteriis, E.D., Parascandola, P., Pecorella, M.A., Scardi, V. (1987). Entrapment of microbial cells within a gelatin matrix: A comparison of three procedures. Biotechnology Techniques, 1(2), 109-114. DOI: 10.1007/bf00159331
  31. Munjal, N., Sawhney, S. (2002). Stability and properties of mushroom tyrosinase entrapped in alginate, polyacrylamide and gelatin gels. Enzyme and Microbial Technology, 30(5), 613–619. DOI: 10.1016/s0141-0229(02)00019-4
  32. Rushan, N.H., Yasin, N.H.M., Sepian, N.R.A., Said, F.M., Shafei, N.I. (2019). Effect of Immobilization Method on the Growth of Chlorella vulgaris and Fatty Acid Profile for Biodiesel Production. Indonesian Journal of Chemistry, 19(3), 767-776. DOI: 10.22146/ijc.39800
  33. Kumar, B.R., Deviram, G., Mathimani, T., Duc, P.A., Pugazhendhi, A. (2019). Microalgae as rich source of polyunsaturated fatty acids. Biocatalysis and agricultural biotechnology, 17, 583-588. DOI: 10.1016/j.bcab.2019.01.017
  34. Mathimani, T., Baldinelli, A., Rajendran, K., Prabakar, D., Matheswaran, M., van Leeuwen, R.P., Pugazhendhi, A. (2019). Review on cultivation and thermochemical conversion of microalgae to fuels and chemicals: process evaluation and knowledge gaps. Journal of cleaner production, 208, 1053-1064. DOI: 10.1016/j.jclepro.2018.10.096
  35. Linares, D.M., Gómez, C., Renes, E., Fresno, J.M., Tornadijo, M.E., Ross, R.P., Stanton, C. (2017). Lactic Acid Bacteria and Bifidobacteria with Potential to Design Natural Biofunctional Health-Promoting Dairy Foods. Frontiers in Microbiology, 8, 846, DOI: 10.3389/fmicb.2017.00846
  36. Sharma, J., Kumar, V., Kumar, S.S., Malyan, S.K., Mathimani, T., Bishnoi, N.R., Pugazhendhi, A. (2020). Microalgal consortia for municipal wastewater treatment–Lipid augmentation and fatty acid profiling for biodiesel production. Journal of Photochemistry and Photobiology B: Biology, 202, 111638. DOI: 1016/j.jphotobiol.2019.111638
  37. Idris, N.A. (2017). Cultivation of Microalgae in Medium Containing Palm Oil Mill Effluent and Its Conversion into Biofuel. Journal of Oil Palm Research, 29(2), 291-299. DOI: 10.21894/jopr.2017.2902.13
  38. Yeh, K., Chang, J. (2012). Effects of cultivation conditions and media composition on cell growth and lipid productivity of indigenous microalga Chlorella vulgaris ESP-31. Bioresource Technology, 105, 120-127. DOI: 10.1016/j.biortech.2011.11.103

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