Synthesis of Chitosan/Zinc Oxide Nanoparticles Stabilized by Chitosan via Microwave Heating

*Nurul Amira Ahmad Yusof  -  Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pa, Malaysia
Norashikin Mat Zain  -  Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pa, Malaysia
Norlin Pauzi  -  Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pa, Malaysia
Received: 1 Oct 2018; Revised: 15 Feb 2019; Accepted: 15 Feb 2019; Published: 1 Aug 2019; Available online: 30 Apr 2019.
Open Access Copyright (c) 2019 Bulletin of Chemical Reaction Engineering & Catalysis
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Nowadays, zinc oxide (ZnO) has attracted attention in research and development because of its remarkable antibacterial properties. Chitosan/ZnO nanoparticles were successfully synthesized via microwave heating. The objectives of this work were to investigate the effect of stabilizer, power heating and time heating on size of chitosan/ZnO nanoparticles and to determine antibacterial activity against pathogenic bacteria, where chitosan was used as a stabilizing agent. Chitosan/ZnO nanoparticles were analyzed  by Fourier Transform Infra Red (FTIR), X-ray Diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM), and Zetasizer instrument. The power heating and time heating were varied from 400 to 800 Watt and 4 to 8 minutes, respectively. The presence of chitosan has role on preventing the nanoparticles from agglomeration by producing a milky solution of chitosan/ZnO nanoparticles without any suspensions. The increase of power  and time heating improved the size of nanoparticles. The peak in FTIR spectrum at around 427 cm-1 was confirmed the existence of the ZnO phase. XRD patterns showed that the chitosan/ZnO nanoparticles materials were pure phase with average crystalline size is 130 nm. FESEM revealed that chitosan/ZnO nanoparticles were uniformly distributed with the mean value of size is 70 nm and spherical shaped. Further impact of power and time heating on the size of the chitosan/ZnO nanoparticles can be shown by a nanoparticles size distribution with the average of 30 to 90 nm. The results showed that chitosan/ZnO nanoparticles have displayed an antibacterial inhibition zone against Gram-positive S. aureus and Gram-negative E. coli which 16.0 and 13.3 mm, respectively. Chitosan/ZnO nanoparticles were synthesized in this work presented have potential application to prevent bacterial infections. Copyright © 2019 BCREC Group. All rights reserved


Keywords: ZnO Nanoparticles; Chitosan; Microwave Heating; Stabilizer; Antibacterial
Funding: Ministry of Education, Malaysia, and Universiti Malaysia Pahang for financial support (RDU 150333)

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