skip to main content

Microwave Irradiation-Assisted Chitosan Hydrolysis Using Cellulase Enzyme

1Department of Chemical Engineering, Faculty of Engineering, Diponegoro University, Indonesia

2Department of Environmental Engineering, Faculty of Engineering, Diponegoro University, Indonesia

Received: 19 Mar 2018; Revised: 19 Jun 2018; Accepted: 25 Jun 2018; Published: 4 Dec 2018; Available online: 14 Nov 2018.
Open Access Copyright (c) 2018 by Authors, Published by BCREC Group under http://creativecommons.org/licenses/by-sa/4.0.

Citation Format:
Cover Image
Abstract

The influence of microwave irradiation on the chitosan hydrolysis catalyzed by cellulase enzyme was studied. The hydrolyzed chitosan was characterized by measuring its viscosity and reducing sugar. Further, it was also characterized by Fourier-Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD), and Scanning Electron Microscope (SEM). The classical Michaelis-Menten kinetic parameters were measured by analyzing the amount of reducing sugars. The results were compared with the hydrolysis by using conventional shaker incubator. The hydrolysis reaction time needed to obtain similar reducing sugar yield was significantly lower for microwave irradiation than shaker incubator. On the other hand, the reduction rate of the relative viscosity was significantly higher for the hydrolysis of chitosan using shaker incubator. A significant difference in chemical structure was observed between hydrolysis using microwave irradiation and shaker incubator. Overall, the result showed that the hydrolysis behavior of chitosan using microwave irradiation is significantly different with using shaker incubator. 

 

Fulltext View|Download
Keywords: Microwave Irradiation; Hydrolysis; Chitosan; Cellulase
Funding: Ministry of Research, Technology, and Higher Education, Republic of Indonesia

Article Metrics:

  1. Shahidi, F., Abuzaytoun, R. (2005). Chitin, Chitosan, and Co-Products: Chemistry, Production, Applications, and Health Effects. Advances in Food and Nutrition Research, 49: 93-135
  2. Pillai, C.K.S., Paul, W., Sharma, C.P. (2009). Chitin and Chitosan Polymers: Chemistry, Solubility and Fiber Formation. Progress in Polymer Science, 34(7): 641-678
  3. Prashanth, K., Tharanathan, R.N. (2007). Chitin/chitosan: Modifications and their Unlimited Application Potential-An Overview. Trends in Food Science & Technology, 18(3): 117-131
  4. Rinaudo, M. (2006). Chitin and Chitosan: Properties and Applications. Progress in Polymer Science, 31(7): 603-632
  5. Huang, K.S., Wu, W.J., Chen, J.B., Lian, H.S. (2008). Application of Low-Molecular-Weight Chitosan in Durable Press Finishing. Carbohydrate Polymers, 73(2): 254-260
  6. Dash, M., Chiellini, F., Ottenbrite, R.M., Chiellini E. (2011). Chitosan-A Versatile Semi-synthetic Polymer in Biomedical Applications. Progress in Polymer Science, 36(8): 981-1014
  7. Anitha, A., Sowmya, S., Kumar, P.S., Deepthi, S., Chennazhi, K.P., Ehrlich, H., Tsurkan, M., Jayakumar, R. (2014). Chitin and Chitosan in Selected
  8. Biomedical Applications. Progress in Polymer Science, 39(9): 1644-1667
  9. Tsao, C.T., Chang, C.H., Lin, Y.Y., Wu, M.F., Han, J.L., Hsieh, K.H. (2011). Kinetic Study of Acid Depolymerization of Chitosan and Effects of Low Molecular Weight Chitosan on Erythrocyte Rouleaux Formation. Carbohydrate Research, 346(1): 94-102
  10. Zhang, Z., Li, C., Wang, Q., Zhao, Z.K. (2009). Efficient Hydrolysis of Chitosan in Ionic Liquids. Carbohydrate Polymers, 78(4): 685-689
  11. Roncal, T., Oviedo, A., de Armentia, I.L., Fernández, L., Villarán, M.C. (2007). High Yield Production of Monomer-Free Chitosan Oligosaccharides by Pepsin Catalyzed Hydrolysis of a High Deacetylation Degree Chitosan. Carbohydrate Research, 342(18): 2750-2756
  12. Cabrera, J.C., Van Cutsem, P. (2005). Preparation of Chitooligosaccharides with Degree of Polymerization Higher than 6 by Acid or Enzymatic Degradation of Chitosan. Biochemical Engineering Journal, 25(2): 165-172
  13. Lee, D.X., Xia, W.S., Zhang, J.L. (2008). Enzymatic Preparation of Chitooligosaccharides by Commercial Lipase. Food Chemistry, 111(2): 291-295
  14. Li, J.F., Wei, F., Dong, X.Y., Guo, L.L., Yuan, G.Y., Huang, F.H., Jiang, M.L., Zhao, Y.D., Li, G.M., Chen, H. (2010). Microwave-assisted Approach for the Rapid Enzymatic Digestion of Rapeseed Meal. Food Science and Biotechnology, 19(2): 463-469
  15. Warrand, J., Janssen, H.G. (2007). Controlled Production of Oligosaccharides from Amylose by Acid-Hydrolysis under Microwave Treatment: Comparison with Conventional Heating. Carbohydrate Polymers, 69(2): 353-362
  16. Li, K., Xing, R., Liu, S., Qin, Y., Meng, X., Li, P. (2012). Microwave-assisted Degradation of Chitosan for a Possible Use in Inhibiting Crop Pathogenic Fungi. International Journal of Biological Macromolecules, 51(5): 767-773
  17. Saxena, R.K., Isar, J., Saran, S., Kaushik, R., Davidson, W.S. (2005). Efficient Microwave-assisted Hydrolysis of Triolein and Synthesis of Bioester, Biosurfactant and Glycerides using Aspergillus carneus Lipase. Current Science, 89(6): 1000-1003
  18. Xia, W., Liu, P., Liu, J. (2008). Advance in Chitosan Hydrolysis by Non-specific Cellulases. Bioresource Technology, 99(15): 6751-6762
  19. Klein, B., Vanier, N.L., Moomand, K., Pinto, V.Z., Colussi, R., da Rosa Zavareze, E., Dias, A.R.G. (2014). Ozone Oxidation of Cassava Starch in Aqueous Solution at Different pH. Food Chemistry, 155:167-173
  20. Li, J., Du, Y., Liang, H. (2007). Influence of Molecular Parameters on the Degradation of Chitosan by a Commercial Enzyme. Polymer Degradation and Stability, 92(3): 515-524
  21. Lin, S.B., Lin, Y.C., Chen, H.H. (2009). Low Molecular Weight Chitosan Prepared with the Aid of Cellulase, Lysozyme and Chitinase: Characterisation and Antibacterial Activity. Food Chemistry, 116(1): 47-53
  22. Mello, P.A., Barin, J.S., Guarnieri, R.A. (2014). Microwave Heating. pp.60-75. In: Microwave-Assisted Sample Preparation for Trace Element Determination. Flores EMM (ed). Elsevier, Amsterdam
  23. Galema, S.A. (1997). Microwave Chemistry. Chemical Society Reviews, 26(3): 233-238
  24. Su, P., Wang, S., Shi, Y., Yang, Y. (2013). Application of Cellulase-polyamidoamine Dendrimer-modified Silica for Microwave-assisted Chitosan Enzymolysis. Process Biochemistry, 48(4): 614-619
  25. Kumar, A.B.V., Varadaraj, M.C., Gowda, L.R., Tharanathan, R.N. (2007). Low Molecular Weight Chitosans-preparation with the Aid of Pronase, Characterization and their Bactericidal Activity towards Bacillus Cereus and Escherichia coli. BBA General Subjects, 1770(4): 495-505
  26. Prasertsung, I., Damrongsakkul, S., Saito, N. (2013). Degradation of β-chitosan by Solution Plasma Process (SPP). Polymer Degradation and Stability, 98(10): 2089-2093
  27. Li, J., Du, Y., Yang, J., Feng, T., Li, A., Chen, P. (2005). Preparation and Characterisation of Low Molecular Weight Chitosan and Chito-Oligomers by a Commercial Enzyme. Polymer Degradation and Stability, 87(3): 441-448
  28. Singh, J., Dutta, P.K., Dutta, J., Hunt, A.J., Macquarrie, D.J., Clark, J.H. (2009). Preparation and Properties of Highly Soluble Chitosan–L-glutamic Acid Aerogel Derivative. Carbohydrate Polymers, 76(2): 188-195
  29. Luo, W.B., Han, Z., Zeng, X.A., Yu, S.J., Kennedy, J.F. (2010). Study on the Degradation of Chitosan by Pulsed Electric Fields Treatment. Innovative Food Science and Emerging Technologies. 11(4): 587-591

Last update:

No citation recorded.

Last update:

No citation recorded.