Microwave Irradiation-Assisted Chitosan Hydrolysis Using Cellulase Enzyme

DOI: https://doi.org/10.9767/bcrec.13.3.2378.466-474
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Submitted: 19-03-2018
Published: 04-12-2018
Section: Original Research Articles
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The influence of microwave irradiation on the chitosan hydrolysis catalyzed by cellulase enzyme was studied. The hydrolyzed chitosan was characterized by measuring its viscosity and reducing sugar. Further, it was also characterized by Fourier-Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD), and Scanning Electron Microscope (SEM). The classical Michaelis-Menten kinetic parameters were measured by analyzing the amount of reducing sugars. The results were compared with the hydrolysis by using conventional shaker incubator. The hydrolysis reaction time needed to obtain similar reducing sugar yield was significantly lower for microwave irradiation than shaker incubator. On the other hand, the reduction rate of the relative viscosity was significantly higher for the hydrolysis of chitosan using shaker incubator. A significant difference in chemical structure was observed between hydrolysis using microwave irradiation and shaker incubator. Overall, the result showed that the hydrolysis behavior of chitosan using microwave irradiation is significantly different with using shaker incubator. Copyright © 2018 BCREC Group. All rights reserved

Received: 19th March 2018; Revised: 19th June 2018; Accepted: 25th June 2018

How to Cite: Rokhati, N., Pramudono, B., Istirokhatun, T., Susanto, H. (2018). Microwave Irradiation-Assisted Chitosan Hydrolysis Using Cellulase Enzyme. Bulletin of Chemical Reaction Engineering & Catalysis, 13 (3): 466-474 (doi:10.9767/bcrec.13.3.2378.466-474)

Permalink/DOI: https://doi.org/10.9767/bcrec.13.3.2378.466-474

 

Keywords

Microwave Irradiation; Hydrolysis; Chitosan; Cellulase

  1. Nur Rokhati 
    Department of Chemical Engineering, Faculty of Engineering, Diponegoro University, Indonesia
  2. Bambang Pramudono 
    Department of Chemical Engineering, Faculty of Engineering, Diponegoro University, Indonesia
  3. Titik Istirokhatun 
    Department of Environmental Engineering, Faculty of Engineering, Diponegoro University, Indonesia
  4. Heru Susanto  Orcid Scopus Scholar Sinta
    Department of Chemical Engineering, Faculty of Engineering, Diponegoro University, Indonesia
  1. Shahidi, F., Abuzaytoun, R. (2005). Chitin, Chitosan, and Co-Products: Chemistry, Production, Applications, and Health Effects. Advances in Food and Nutrition Research, 49: 93-135.
  2. Pillai, C.K.S., Paul, W., Sharma, C.P. (2009). Chitin and Chitosan Polymers: Chemistry, Solubility and Fiber Formation. Progress in Polymer Science, 34(7): 641-678.
  3. Prashanth, K., Tharanathan, R.N. (2007). Chitin/chitosan: Modifications and their Unlimited Application Potential-An Overview. Trends in Food Science & Technology, 18(3): 117-131.
  4. Rinaudo, M. (2006). Chitin and Chitosan: Properties and Applications. Progress in Polymer Science, 31(7): 603-632.
  5. Huang, K.S., Wu, W.J., Chen, J.B., Lian, H.S. (2008). Application of Low-Molecular-Weight Chitosan in Durable Press Finishing. Carbohydrate Polymers, 73(2): 254-260.
  6. Dash, M., Chiellini, F., Ottenbrite, R.M., Chiellini E. (2011). Chitosan-A Versatile Semi-synthetic Polymer in Biomedical Applications. Progress in Polymer Science, 36(8): 981-1014.
  7. Anitha, A., Sowmya, S., Kumar, P.S., Deepthi, S., Chennazhi, K.P., Ehrlich, H., Tsurkan, M., Jayakumar, R. (2014). Chitin and Chitosan in Selected
  8. Biomedical Applications. Progress in Polymer Science, 39(9): 1644-1667.
  9. Tsao, C.T., Chang, C.H., Lin, Y.Y., Wu, M.F., Han, J.L., Hsieh, K.H. (2011). Kinetic Study of Acid Depolymerization of Chitosan and Effects of Low Molecular Weight Chitosan on Erythrocyte Rouleaux Formation. Carbohydrate Research, 346(1): 94-102.
  10. Zhang, Z., Li, C., Wang, Q., Zhao, Z.K. (2009). Efficient Hydrolysis of Chitosan in Ionic Liquids. Carbohydrate Polymers, 78(4): 685-689.
  11. Roncal, T., Oviedo, A., de Armentia, I.L., Fernández, L., Villarán, M.C. (2007). High Yield Production of Monomer-Free Chitosan Oligosaccharides by Pepsin Catalyzed Hydrolysis of a High Deacetylation Degree Chitosan. Carbohydrate Research, 342(18): 2750-2756.
  12. Cabrera, J.C., Van Cutsem, P. (2005). Preparation of Chitooligosaccharides with Degree of Polymerization Higher than 6 by Acid or Enzymatic Degradation of Chitosan. Biochemical Engineering Journal, 25(2): 165-172.
  13. Lee, D.X., Xia, W.S., Zhang, J.L. (2008). Enzymatic Preparation of Chitooligosaccharides by Commercial Lipase. Food Chemistry, 111(2): 291-295.
  14. Li, J.F., Wei, F., Dong, X.Y., Guo, L.L., Yuan, G.Y., Huang, F.H., Jiang, M.L., Zhao, Y.D., Li, G.M., Chen, H. (2010). Microwave-assisted Approach for the Rapid Enzymatic Digestion of Rapeseed Meal. Food Science and Biotechnology, 19(2): 463-469.
  15. Warrand, J., Janssen, H.G. (2007). Controlled Production of Oligosaccharides from Amylose by Acid-Hydrolysis under Microwave Treatment: Comparison with Conventional Heating. Carbohydrate Polymers, 69(2): 353-362.
  16. Li, K., Xing, R., Liu, S., Qin, Y., Meng, X., Li, P. (2012). Microwave-assisted Degradation of Chitosan for a Possible Use in Inhibiting Crop Pathogenic Fungi. International Journal of Biological Macromolecules, 51(5): 767-773.
  17. Saxena, R.K., Isar, J., Saran, S., Kaushik, R., Davidson, W.S. (2005). Efficient Microwave-assisted Hydrolysis of Triolein and Synthesis of Bioester, Biosurfactant and Glycerides using Aspergillus carneus Lipase. Current Science, 89(6): 1000-1003.
  18. Xia, W., Liu, P., Liu, J. (2008). Advance in Chitosan Hydrolysis by Non-specific Cellulases. Bioresource Technology, 99(15): 6751-6762.
  19. Klein, B., Vanier, N.L., Moomand, K., Pinto, V.Z., Colussi, R., da Rosa Zavareze, E., Dias, A.R.G. (2014). Ozone Oxidation of Cassava Starch in Aqueous Solution at Different pH. Food Chemistry, 155:167-173.
  20. Li, J., Du, Y., Liang, H. (2007). Influence of Molecular Parameters on the Degradation of Chitosan by a Commercial Enzyme. Polymer Degradation and Stability, 92(3): 515-524.
  21. Lin, S.B., Lin, Y.C., Chen, H.H. (2009). Low Molecular Weight Chitosan Prepared with the Aid of Cellulase, Lysozyme and Chitinase: Characterisation and Antibacterial Activity. Food Chemistry, 116(1): 47-53.
  22. Mello, P.A., Barin, J.S., Guarnieri, R.A. (2014). Microwave Heating. pp.60-75. In: Microwave-Assisted Sample Preparation for Trace Element Determination. Flores EMM (ed). Elsevier, Amsterdam.
  23. Galema, S.A. (1997). Microwave Chemistry. Chemical Society Reviews, 26(3): 233-238.
  24. Su, P., Wang, S., Shi, Y., Yang, Y. (2013). Application of Cellulase-polyamidoamine Dendrimer-modified Silica for Microwave-assisted Chitosan Enzymolysis. Process Biochemistry, 48(4): 614-619.
  25. Kumar, A.B.V., Varadaraj, M.C., Gowda, L.R., Tharanathan, R.N. (2007). Low Molecular Weight Chitosans-preparation with the Aid of Pronase, Characterization and their Bactericidal Activity towards Bacillus Cereus and Escherichia coli. BBA General Subjects, 1770(4): 495-505.
  26. Prasertsung, I., Damrongsakkul, S., Saito, N. (2013). Degradation of β-chitosan by Solution Plasma Process (SPP). Polymer Degradation and Stability, 98(10): 2089-2093.
  27. Li, J., Du, Y., Yang, J., Feng, T., Li, A., Chen, P. (2005). Preparation and Characterisation of Low Molecular Weight Chitosan and Chito-Oligomers by a Commercial Enzyme. Polymer Degradation and Stability, 87(3): 441-448.
  28. Singh, J., Dutta, P.K., Dutta, J., Hunt, A.J., Macquarrie, D.J., Clark, J.H. (2009). Preparation and Properties of Highly Soluble Chitosan–L-glutamic Acid Aerogel Derivative. Carbohydrate Polymers, 76(2): 188-195.
  29. Luo, W.B., Han, Z., Zeng, X.A., Yu, S.J., Kennedy, J.F. (2010). Study on the Degradation of Chitosan by Pulsed Electric Fields Treatment. Innovative Food Science and Emerging Technologies. 11(4): 587-591.