Solid State Fermentation Parameters Effect on Cellulase Production from Empty Fruit Bunch
Copyright (c) 2018 Bulletin of Chemical Reaction Engineering & Catalysis
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Article Metrics: (Click on the Metric tab below to see the detail)
In this study, agriculture waste palm empty fruit bunch (EFB) was used as carbon/cellulose source in solid state fermentation for cheaper cellulase production. Fermentation operation parameters, such as: solid to liquid ratio, temperature, and pH, were varied to study the effect of those parameters towards crude cellulase activity. Two different fungi organisms, Trichoderma viride and Trichoderma reesei were used as the producers. Extracellular cellulase enzyme was extracted using simple contact method using citrate buffer. Assessment of the extracted cellulase activity by filter paper assay showed that Trichoderma viride is the superior organism capable of producing higher cellulase amount compared to Trichoderma reesei at the same fermentation condition. The optimum cellulase activity of 0.79 FPU/g dry substrate was obtained when solid to liquid ratio used for the fermentation was 1:1, while the optimum fermentation temperature and pH were found to be 30 °C and 5.5, respectively. The result obtained in this research showed the potential of EFB utilization for enzyme production. Copyright © 2018 BCREC Group. All rights reserved
Received: 14th December 2017; Revised:29th July 2018; Accepted: 3rd August 2018
How to Cite: Wonoputri, V., Subiantoro, S., Kresnowati, M.T.A.P., Purwadi, R. (2018). Solid State Fermentation Parameters Effect on Cellulase Production from Empty Fruit Bunch. Bulletin of Chemical Reaction Engineering & Catalysis, 13 (3): 553-559 (doi:10.9767/bcrec.13.3.1964.553-559)
- Yoon, L.W., Ang, T.N., Ngoh, G.C., Chua, A.S.M. (2014). Fungal Solid-State Fermentation and Various Methods of Enhancement in Cellulase Production. Biomass and Bioenergy, 67: 319–338.
- Ellilä, S., Fonseca, L., Uchima, C., Cota, J., Goldman, G.H., Saloheimo, M., Sacon, V., Siika, M. (2017). Development of a Low‑Cost Cellulase Production Process using Trichoderma reesei for Brazilian Biorefineries. Biotechnology for Biofuels, 10: 1-17.
- Kuhad, R.C., Deswal, D., Sharma, S., Bhattacharya, A., Jain, K.K, Kaur, A., Pletschke, B.I., Singh, A., Karp, M. (2016). Revisiting Cellulase Production and Redefining Current Strategies Based on Major Challenges. Renewable and Sustainable Energy Reviews, 55: 249–272.
- Klein-Marcuschamer, D., Oleskowicz-Popiel, P., Simmons, B.A., Blanch, H.W. (2012). The Challenge of Enzyme Cost in the Production of Lignocellulosic Biofuels. Biotechnology and Bioengineering, 109: 1083–1087.
- Behera, S.S., Ray, R.C. (2016). Solid State Fermentation for Production of Microbial Cellulases: Recent Advances and Improvement Strategies. International Journal of Biological Macromolecules, 86: 656–669.
- Cerda, A., Gea, T., Vargas-García, M.C., Sánchez, A. (2017). Towards a Competitive Solid State Fermentation: Cellulases Production from Coffee Husk by Sequential Batch Operation and Role of Microbial Diversity. Science of the Total Environment, 589: 56–65.
- Farinas, C.S. (2015). Developments in Solid-State Fermentation for the Production of Biomass-Degrading Enzymes for the Bioenergy Sector. Renewable and Sustainable Energy Reviews, 52: 179–188.
- Kim, S., Ho, C. (2012). Production of Cellulase Enzymes During the Solid-State Fermentation of Empty Palm Fruit Bunch Fiber. Bioprocess and Biosystems Engineering, 35: 61–67.
- Alam, M.Z., Mamun, A.A., Qudsieh, I.Y., Muyibi, S.A, Salleh, H.M., Omar, N.M. (2009) Solid State Bioconversion of Oil Palm Empty Fruit Bunches for Cellulase Enzyme Production using a Rotary Drum Bioreactor. Biochemical Engineering Journal, 46: 61–64.
- Wang, Z., Ong, H.X., Geng, A. (2012) Cellulase Production and Oil Palm Empty Fruit Bunch Saccharification by a New Isolate of Trichoderma koningii D-64. Process Biochemistry, 47: 1564–1571.
- Nuñez-Gaona, O., Saucedo-Castañeda, G., Alatorre-Rosas, R., Loera, O. (2010). Effect of Moisture Content and Inoculum on the Growth and Conidia Production by Beauveria bassiana on Wheat Bran. Brazilian Archives of Biology and Technology, 53: 771–777.
- Sugiharto, Y.E.C., Harimawan, A., Kresnowati, M.T.A.P., Purwadi, R., Mariyana, R., Andry, Fitriana, H.N., Hosen, H.F. (2016). Enzyme Feeding Strategies for Better Fed-Batch Enzymatic Hydrolysis of Empty Fruit Bunch. Bioresource Technology, 207: 175–179.
- Chahal, D.S. (1985). Solid-State Fermentation with Trichoderma reesei for Cellulase Production. Applied and Environment Microbiology, 49: 205–210.
- Adney, B., Baker, J. (2008). Measurement of Cellulase Activities Laboratory Analytical Procedure.
- Ghose, T.K. (1987). Measurement of Cellulase Activities. Pure and Applied Chemistry, 59: 257-268
- Piarpuzán, D., Quintero, J.A., Cardona, C.A. (2011). Empty Fruit Bunches from Oil Palm as a Potential Raw Material for Fuel Ethanol Production. Biomass and Bioenergy, 35: 1130–1137.
- Wen, Z., Liao, W., Chen, S. (2005). Production of Cellulase by Trichoderma reesei from Dairy Manure. Bioresource Technology, 96: 491–499.
- Gomes, I., Shaheen, M., Rahman, S.R., Gomes, D.J. (2006). Comparative Studies on Production of Cell Wall-Degrading Hydrolases by Trichoderma reesei and T. viride in Submerged and Solid-State Cultivations. Bangladesh Journal of Microbiology, 23: 149–155.
- Singhania, R.R., Sukumaran, R.K., Pandey, A. (2007). Improved Cellulase Production by Trichoderma reesei RUT C30 under SSF Through Process Optimization. Applied Biochemistry and Biotechnology, 142: 60–70.
- Latifian, M., Hamidi-Esfahani, Z., Barzegar, M. (2007). Evaluation of Culture Conditions for Cellulase Production by Two Trichoderma reesei Mutants under Solid-State Fermentation Conditions, Bioresource Technology, 98: 3634–3637.
- Shahriarinour, M., Wahab, M. (2013) Effect of Medium Composition and Cultural Condition on Cellulase Production by Aspergillus terreus. African Journal of Biotechnology, 10: 7459–7467.
- Idris, A.S.O., Pandey, A., Rao, S.S., Sukumaran, R.K. (2017). Cellulase Production through Solid-State Tray Fermentation, and Its Use for Bioethanol from Sorghum Stover. Bioresource Technology, 242: 265–271.
- Vitcosque, G.L., Fonseca, R.F., Rodríguez-Zúñiga, U.F., Neto V.B., Couri, S., Farinas, C.S. (2012). Production of Biomass-Degrading Multienzyme Complexes under Solid-State Fermentation of Soybean Meal Using a Bioreactor. Enzyme Research, vol. 2012, Article ID 248983, 9 pages. DOI: 10.1155/2012/248983.
- Pensupa, N., Jin, M., Kokolski, M., Archer, D.B., Du, C. (2013). A Solid State Fungal Fermentation-Based Strategy for the Hydrolysis of Wheat Straw. Bioresource Technology, 149: 261–267.
- Djakaria, B. (2017). Optimization of Xylanase Production Using Empty Fruit Bunches by Solid-State Fermentation in a Tray Bioreactor. Master Thesis, Department of Chemical Engineering, Institut Teknologi Bandung.
- Singhania, R.R., Sukumaran, R.K., Pillai, A., Prema, P., Szakacs, G., Pandey, A. (2006). Solid-State Fermentation of Lignocellulosic Substrates for Cellulase Production by Trichoderma reesei NRRL11460. Indian Journal of Biotechnology, 5: 332–336.
- Gomes, I., Gomes, J., Steiner, W., Esterbauer, H. Production of Cellulase and Xylanase by a Wild Strain of Trichoderma viride. Applied Microbiology and Biotechnology, 36: 701–707.
- Xia, L., Cen, P. (1999). Cellulase Production by Solid State Fermentation on Lignocellulosic Waste from The Xylose industry, Process Biochemistry, 34: 909–912.
- Sukumaran, R.K., Singhania, R.R., Mathew, G.M., Pandey, A. (2009). Cellulase Production Using Biomass Feed Stock and its Application in Lignocellulose Saccharification for Bio-Ethanol production. Renewable Energy, 34: 421–424.
- Lever, M., Ho, G., Cord-Ruwisch, R. (2010). Ethanol from Lignocellulose Using Crude Unprocessed Cellulase from Solid-State Fermentation. Bioresource Technology, 101: 7083–7087.
- Behera, A., Arora, R., Nandhagopal, N., Kumar, S. (2014). Importance of Chemical Pretreatment for Bioconversion of Lignocellulosic Biomass. Renewable and Sustainable Energy Reviews, 36: 91–106.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors and readers can copy and redistribute the material in any medium or format, as well as remix, transform, and build upon the material for any purpose, even commercially, but they must give appropriate credit (cite to the article or content), provide a link to the license, and indicate if changes were made. If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
Copyright Transfer Agreement
The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Bulletin of Chemical Reaction Engineering & Catalysis journal and Department of Chemical Engineering Diponegoro University as publisher of the journal.
Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc., will be allowed only with a written permission from Bulletin of Chemical Reaction Engineering & Catalysis journal and Department of Chemical Engineering Diponegoro University.
Bulletin of Chemical Reaction Engineering & Catalysis journal and Department of Chemical Engineering Diponegoro University, the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal. In any way, the contents of the articles and advertisements published in the Bulletin of Chemical Reaction Engineering & Catalysis are sole and exclusive responsibility of their respective authors and advertisers.
The Copyright Transfer Form can be downloaded here: [Copyright Transfer Form BCREC 2016]
The copyright form should be signed originally and send to the Editorial Office in the form of original mail, scanned document or fax :
Prof. Dr. I. Istadi (Editor-in-Chief)
Editorial Office of Bulletin of Chemical Reaction Engineering and Catalysis
Department of Chemical Engineering, Diponegoro University
Jl. Prof. Soedarto, Kampus Undip Tembalang, Semarang, Central Java, Indonesia 50275
Telp.: +62-24-7460058, Fax.: +62-24-76480675