Solid State Fermentation Parameters Effect on Cellulase Production from Empty Fruit Bunch

DOI: https://doi.org/10.9767/bcrec.13.3.1964.553-559
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Submitted: 14-12-2017
Published: 04-12-2018
Section: Original Research Articles
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In this study, agriculture waste palm empty fruit bunch (EFB) was used as carbon/cellulose source in solid state fermentation for cheaper cellulase production. Fermentation operation parameters, such as: solid to liquid ratio, temperature, and pH, were varied to study the effect of those parameters towards crude cellulase activity. Two different fungi organisms, Trichoderma viride and Trichoderma reesei were used as the producers. Extracellular cellulase enzyme was extracted using simple contact method using citrate buffer. Assessment of the extracted cellulase activity by filter paper assay showed that Trichoderma viride is the superior organism capable of producing higher cellulase amount compared to Trichoderma reesei at the same fermentation condition. The optimum cellulase activity of 0.79 FPU/g dry substrate was obtained when solid to liquid ratio used for the fermentation was 1:1, while the optimum fermentation temperature and pH were found to be 30 °C and 5.5, respectively. The result obtained in this research showed the potential of EFB utilization for enzyme production. Copyright © 2018 BCREC Group. All rights reserved

Received: 14th December 2017; Revised:29th July 2018; Accepted: 3rd August 2018

How to Cite: Wonoputri, V., Subiantoro, S., Kresnowati, M.T.A.P., Purwadi, R. (2018). Solid State Fermentation Parameters Effect on Cellulase Production from Empty Fruit Bunch. Bulletin of Chemical Reaction Engineering & Catalysis, 13 (3): 553-559 (doi:10.9767/bcrec.13.3.1964.553-559)

Permalink/DOI: https://doi.org/10.9767/bcrec.13.3.1964.553-559

 

Keywords

Cellulase; Empty Fruit Bunch; Trichoderma; Solid State Fermentation

  1. Vita Wonoputri  Scopus
    Department of Chemical Engineering, Faculty of Industrial Technology, Institut Teknologi Bandung , Ganesa 10, Bandung 40132, Indonesia
    Lecturer
  2. Subiantoro Subiantoro 
    Department of Chemical Engineering, Faculty of Industrial Technology, Institut Teknologi Bandung , Ganesa 10, Bandung 40132, Indonesia
  3. Made Tri Ari Penia Kresnowati 
    Department of Chemical Engineering, Faculty of Industrial Technology, Institut Teknologi Bandung , Ganesa 10, Bandung 40132, Indonesia
  4. Ronny Purwadi 
    Department of Chemical Engineering, Faculty of Industrial Technology, Institut Teknologi Bandung , Ganesa 10, Bandung 40132, Indonesia
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