DOI: https://doi.org/10.14710/aesj.v2i1.18119

Assessment of BAP and NAA on In vitro Culture of Vanilla (Vanilla planifolia Andrews.) at The Final Stage of Subculture

*N. H. Andriyani orcid  -  Department of Agriculture, Faculty of Animal and Agricultural Science, Universitas Diponegoro, Jl. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275, Indonesia
S. Anwar  -  Department of Agriculture, Faculty of Animal and Agricultural Science, Universitas Diponegoro, Jl. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275, Indonesia
F. Kusmiyati  -  Department of Agriculture, Faculty of Animal and Agricultural Science, Universitas Diponegoro, Jl. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275, Indonesia

Citation Format:
Abstract

The purpose of research was to examine BAP and NAA on in vitro culture of vanilla at final stage of subculture. The research was carried out in October 2021 – January 2022 at the Tissue Culture Laboratory of Balai Benih Tanaman Perkebunan, Salatiga. This research used a 3x4 factorial complete randomized design with 4 replicates. The first factor is the BAP concentration levels at 0, 1 and 2 mg/l. The second factor is the concentration of NAA at 0, 1, 2 and 3 mg/l. The parameters of initiation root period, number of roots, root length, shoot length and number of leaves obtained were analyzed for variance; if it had an effect, it was continued with DMRT. Data on the number of shoots was analyzed descriptively. The results showed an interaction between the additions of BAP and NAA on root length and number of leaves. The addition of BAP significantly affects the number of roots and shoot length, and the highest results were achieved at concentration of 0 mg/l. The NAA concentration had a significant effect on the initiation root period and shoot length that was best achieved at 0 mg/l. 

Fulltext View|Download

Article Metrics:

Article Info
Section: Articles
Language : ID
Recent articles
Effect of Rock Phosphate and Inoculation Arbuscular Mycorhizal Fungi and Rhizobium sp Bacteria on Growth of Soybean (Glycine max L.) Keberhasilan Stek Anggur (Vitis vinifera L.) pada Berbagai Macam Aplikasi Zat Pengatur Tumbuh Alami Dan Asal Bahan Stek The Effects of Time of Scion Defoliation and BAP on the Successful Top Grafting of Durian (Durio zibethinus Murr.) More recent articles
  1. Aprinda, O., L. Lizawati, dan E. Eliyanti. 2022. Induksi akar pada eksplan tunas anggrek (Dendrobium var. Airy Beauty) secara in vitro dengan penambahan naphtalene acetic acid (naa) dan 6-benzyl amino purin (bap). J. Agroecotania. 5(1):27-39. doi: https://doi.org/10.22437/agroecotania.v5i1.22825
  2. Ayele, Y. B., W. Tefera dan K. Bantte. 2017. Enhanced protocol development for in vitro multiplication and rooting of vanilla (Vanilla planifolia Andr.) Clone (Van. 2/05). Biotechnology J. International. 18(3):1-11. doi: https://doi.org/10.9734/BJI/2017/33726
  3. Divakaran, M., K, N. Babu, P. N. Ravindran, K. V. Peter, A. J. Plant dan S. Res. 2015. Biotechnology for micropropagation and enhancing variations in Vanilla. Asian J Plant Sci Res. 5(2):52-62
  4. Erawati, D. N., U. Fisdiana dan M. Kadafi, M. 2020. Respons eksplan vanili (Vanilla planifolia) dengan stimulasi bap dan naa melalui teknik mikropropagasi. J. of Applied Agricultural Sciences. 4(2):146-153. doi: https://doi.org/10.25047/agriprima.v4i2.362
  5. Gantait, S. dan S. Kundu. 2017. In vitro biotechnological approaches on Vanilla planifolia Andrews: advancements and opportunities. Acta Physiologiae Plantarum. 39(9):1-19. doi: https://doi.org/10.1007/s11738-017-2462-1
  6. Hasnu, S. dan B. Tanti. 2022. Multiple shoot induction and regeneration of Vanilla borneensis rolfe-a critically endangered orchid of assam, india. Plant Science Today. 9(1):96-104. doi: https://doi.org/10.14719/pst.1292
  7. Inderiati, S., Ratnawati dan Since. 2019. In vitro propagation of vanilla (Vanilla Planifolia Andr.) on different concentration of cytokinins. J. Ilmiah Terapan Budidaya dan Pengelolaan Tanaman Pertanian dan Perkebunan. 8(1):14-17
  8. Jadid N., T. Nurhidayati dan Priyono. 2015. In vitro clonal propagation of Vanilla planifolia Andrews using microshoot-derived node explants. J. of Applied Environmental and Biological Sciences. 5(6):105-110
  9. Jing, H., dan L. C. Strader. 2019. Interplay of auxin and cytokinin in lateral root development. International J. of Molecular Sciences. 20(3):5-12. doi: https://doi.org/10.3390/ijms20030486
  10. Juras, M. C. R., J. Jorge, R. Pescador, W. D. M. Ferreira, V. Tamaki dan R. M. Suzuki. 2019. In vitro culture and acclimatization of Cattleya xanthina (Orchidaceae), an endangered orchid of the Brazilian Atlantic Rainforest. Rodriguésia, 70:2-11. doi: https://doi.org/10.1590/2175-7860201970014
  11. Kartiman, R., D. Sukma, S. I. Aisyah dan A. Purwito. 2018. Multiplikasi in vitro anggrek hitam (Coelogyne pandurata Lindl.) pada perlakuan kombinasi naa dan bap. J. Bioteknologi dan Biosains Indonesia. 30(5):75-87. doi: https://doi.org/10.29122/jbbi.v5i1.2908
  12. Mangena, P. 2020. Benzyl adenine in plant tissue culture-succinct analysis of the overall influence in soybean [Glycine max (L.) Merrill.] seed and shoot culture establishment. J Biotech Res, 11:23-24
  13. Mariani, T. S., M. Febrina dan A. Wicaksono. 2014. Study on multiplication and germination of protocorm-like bodies (plb) in Phalaenopsis sp. Asian J. of Applied Sciences. 2(3):318-322
  14. Mirani, A. A., A. A. Abul-Soad dan G. S. Markhand. 2017. In vitro rooting of Dendrobium nobile orchid: multiple responses to auxin combinations. Notulae Scientia Biologicae. 9(1):84-88. doi: https://doi.org/10.15835/nsb919894
  15. Muller, D. T., K. Waldie, J. P. Miyawaki, C. W. To, J. J. Melnyk, T. Kieber, Kakimoto dan O. Leyser. Cytokinin is required for escape but not release from auxin mediated apical dominance. The Plant J. 2(82):874–886. doi: https://doi.org/10.1111/tpj.12862
  16. Nuraini, A., E. Aprilia, M. Murgayanti dan A. P. Wulandari. 2022. Pengaruh konsentrasi Benzylaminopurine terhadap pertumbuhan eksplan tunas aksilar rami klon lokal wonosobo secara in vitro. J. Kultivasi, 21(2):166-172. doi: https://doi.org/10.24198/kultivasi.v21i2.36540
  17. Oliveira, S. O. D., R. M. Sayd, T. A. Balzon dan J. E. Scherwinski-Pereira. 2013. A new procedure for in vitro propagation of vanilla (Vanilla planifolia) using a double-phase culture system. Scientia Horticulturae. 161:204-209. doi: https://doi.org/10.1016/j.scienta.2013.06.039
  18. Prabaninggar, R. A., E. R. Sasmita dan E. Wahyurini. 2021. In vitro micro-cutting of vanilla (Vanilla planifolia Andrews.) in different naa and bap. J. Techno. 7(1):27-36
  19. Qomariah, U. K. N. dan E. Semiarti. 2019. Propagasi Dendrobium stratiotes Rchb. f. dengan benziladenin secara in vitro. Agrosaintifika. 1(1):14-21
  20. Ramírez-Mosqueda, M. A., L. G. Iglesias-Andreu, E. Favián-Vega, J. A. Teixeira da Silva, O. R. Leyva-Ovalle dan J. Murguía-González. 2019. Morphogenetic stability of variegated Vanilla planifolia Jacks. plants micropropagated in a temporary immersion system (TIB®). Rendiconti Lincei. Scienze Fisiche e Naturali. 30(3):603-609. doi: https://doi.org/10.1007/s12210-019-00813-9
  21. Schaller, G. E., A. Bishopp dan J. J. Kieber. 2015. The yin-yang of hormones: cytokinin and auxin interactions in plant development. The Plant Cell. 27(1):44-63. doi: https://doi.org/10.1105/tpc.114.133595
  22. Sharma, R. U. B. Y. Dan B. Sunil. 2017. Influence of explants type and plant growth regulators on in vitro multiple shoot regeneration of Vanilla planifolia. International J. of Agricultural Science and Research. 7(2):189-196
  23. Solano, M. C. P., J. S. Ruíz, M. T. G. Arnao, O. C. Castro, M. E. G. Tovar dan J. J. B. Bello. 2019. Evaluation of in vitro shoot multiplication and ISSR marker based assessment of somaclonal variants at different subcultures of vanilla (Vanilla planifolia Jacks). Physiology and Molecular Biology of Plants. 25(2):561-567. doi: https://doi.org/10.1007/s12298-019-00645-9
  24. Tan, B. C., C. F. Chin dan P. Alderson. 2011. An improved plant regeneration of Vanilla planifolia Andrews. Plant Tissue Culture and Biotechnology. 21(1):27-33. doi: https://doi.org/10.3329/ptcb.v21i1.9560
  25. Zuraida, A. R., K. H. F. L. Izzati, O. A. Nazreena, W. S. W. Zaliha, C. M. Z. C. Radziah, Z. Zamri dan S. Sreeramanan. 2013. A simple and efficient protocol for the mass propagation of Vanilla planifolia. American J. of Plant Sciences. 4 (9):1685-1692. doi: https://doi.org/10.4236/ajps.2013.49205

Last update:

No citation recorded.

Last update:

No citation recorded.