Karakteristik morfologi dan sitologi tanaman Sutra Bombay (Portulaca grandiflora Hook) hasil poliploidisasi dengan kolkisin pada berbagai konsentrasi dan frekuensi aplikasi

Betty Perdana Sari, Karno Karno, Syaiful Anwar


DOI: https://doi.org/10.14710/joac.1.2.39-48

Abstract


Moss Rose (Portulaca grandiflora Hook) is one of the ornamental plants and medicinal plants. The purpose of this research was to evaluate cytological and morphological responses of Moss Rose (Portulaca grandiflora) with colcichine treatment. Colcichine treatment was done with drop method technique in different concentrations and different frequency of application. This research used Completely Randomized Design in 4x3 factorial arrangement and used descriptive analysis to compare diploid plant and tetraploid plant characters. The first factor was variation of cholcichine concentration which were K0 (0%), K1 (0.1%), K2 (0.3%), and K3 (0.6%). The second factor was variation of application frequency which were A1 (2 drop per day for a day), A2 (2 drop per day for 2 days), and A3 (2 drop per day for 3 days). The observed parameters were shoot response by colcichine treatment, number of chromosomes, length and width of stomata, stomata density, stem diameter, flower diameter, plant high, fresh weight, number of branches, and number of leaves. The result showed that Moss Rose’s shoot was intolerant with colcichine treatment in high concentration and long time application. The tetraploid plants was randomly produced by a number of treatment which were 0.1% colcichine 2 drops for 3 days, 0.3% colcichine 2 drops for 2 days, 0.6% colcichine 2 drops for 2 days, and 0.6% colcichine 2 drops for 3 days. The shoot’s growth was inhibited. The induced of tetraploid plant in Moss Rose was accompanied by double chromosome number as 2n=4x=36, larger stomata (length and width), lower stomata density, and higher morphological characters. Moss Rose (Portulaca grandiflora Hook) is one of the ornamental plants and medicinal plants. The purpose of this research was to evaluate cytological and morphological responses of Moss Rose (Portulaca grandiflora) with colcichine treatment. Colcichine treatment was done with drop method technique in different concentrations and different frequency of application. This research used Completely Randomized Design in 4x3 factorial arrangement and used descriptive analysis to compare diploid plant and tetraploid plant characters. The first factor was variation of cholcichine concentration which were K0 (0%), K1 (0.1%), K2 (0.3%), and K3 (0.6%). The second factor was variation of application frequency which were A1 (2 drop per day for a day), A2 (2 drop per day for 2 days), and A3 (2 drop per day for 3 days). The observed parameters were shoot response by colcichine treatment, number of chromosomes, length and width of stomata, stomata density, stem diameter, flower diameter, plant high, fresh weight, number of branches, and number of leaves. The result showed that Moss Rose’s shoot was intolerant with colcichine treatment in high concentration and long time application. The tetraploid plants was randomly produced by a number of treatment which were 0.1% colcichine 2 drops for 3 days, 0.3% colcichine 2 drops for 2 days, 0.6% colcichine 2 drops for 2 days, and 0.6% colcichine 2 drops for 3 days. The shoot’s growth was inhibited. The induced of tetraploid plant in Moss Rose was accompanied by double chromosome number as 2n=4x=36, larger stomata (length and width), lower stomata density, and higher morphological characters. 

Full Text:

PDF

References


Anghel A. I., O. T. Olaru, F. Gatea, M. Dinu, R. Viorel, Ancuceanu, and V. Istudor. 2013. Preliminary research on Portulaca Grandiflora Hook. species (Portulacaceae) for therapeutic use. J. Farmacia. 61 (4) : 694-702.

Ariyanto, S. E., dan P. Supriyadi. 2011. Pengaruh kolkisin terhadap fenotipe dan jumlah kromosom jahe (Zingiber officinale Rosc.). ISSN : 1979-6870.

As’sadah, M., T. Rahayu, dan A. Haryati. 2016. Metode pemberian kolkisin terhadap respon morfologis tanaman zaitun (Olea europea). J. Biosaintropis. 2 (1) :46-52.

Ciuffo, M. and M. Turina. 2004. A potexvirus related to Papaya mosaic virus isolated from moss rose in Italy. J. Plant Pathology. 53:515.

Coelho, A. A. O. P., A. M. Giulietti, R. M. Harley, and J. C. Yesilyurt. Synonymies and typifications in Portulaca (Portulacaceae) of Brazil. Kew Bulletin. 65 : 37–43.

Cristinet, L. Fre´de´ric X. Burdet, M. Zaiko, U. Hinz, and J. Zry. 2004. Characterizatin and identification of a novel plant extradiol 4,5 dyoxigenase involved with betatalain pigment byosynthesis in Portulaca grandifora. J. Plant Physiology. 134 : 256-274.

Daryono, B. S. dan W. D. Rahmadani. 2009. Karakter fenotipe tanaman krisan (Dendranthema grandiflorum) kultivar big yellow hasil perlakuan kolkisin. J. Agrotropika. 14 (1): 15 – 18.

Ghaffari, S. M. 2006. Occurrence of diploid and polyploid microspores in Sorghum bicolor (Poaceae) is the result of cytomixis. African Journal of Biotechnology. 5 (16) : 1450-1453.

Głowacka, K., S. Jez˙owski, and Z. Kaczmarek. 2011. In vitro induction of polyploidy by colchicine treatment of shoots and preliminary characterisation of induced polyploids in two Miscanthus species. J. Industrial Crops and Products. 32 : 88–96.

Guojun, Z. L. Yun, L. F. Ping, X. Z. He, S. Y. Han. 2009. Effects of root age on biomass and leaf nutrition in tetraploid Robinia pseudoacacia. Journal of Beijing Forestry University. 31 (3) : 37-41.

Haryanti, S., R. B. Hastuti, , N. Setiari, dan A. Banowo. 2009. Pengaruh kolkisin terhadap pertumbuhan, ukuran sel metafase dan kandungan protein biji tanaman kacang hijau (Vigna Radiata (L) Wilczek). J. Penelitian Sains & Teknologi. 10 (2) : 112 – 120.

Jain, A. K. and M. Bashir. 2010. In-vitro propagation of a medicinal plant Portulaca grandiflora. Hook. World Journal of Agricultural Sciences. 6 (3): 327-330.

Leitch, A. R. dan I. J Leitch. 2008. Genomic plasticity and the diversity of polyploid plants. J. Science. 320 (5) : 481-483.

Liu, G., Z. Li, and M. Bao. 2007. Colchicine-induced Chromosome Doubling in Platanus acerifolia and its effect on plant morphology. International Journal of Plant Breeding. 157 (1):145-154.

Matthews, J. F., W. D. Ketron, and S. F. Zane. 1994. The seed surface morphology and cytology of six species of Portulaca (Portulacaceae). J. Castane. 59 (4) : 331-337.

Mumpuni, M., T. Chikmawati dan T. N. Praptosuwiryo. 2015. Poliploidi intraspesifik Pteris Vittata L. (Pteridaceae) di Pulau Jawa. J. Floribunda. 5 (2) : 53-59.

Murni. 2010. Pengaruh perlakuan kolkisin terhadap jumlah kromosom dan fenotip tanaman cabe keriting (Capsicum annuum L.). J. Agroekotek. 2 (1) : 43-48.

Nofitahesti, I., dan B. S. Daryono. 2016. Karakter fenotip kedelai (Glycine Max (L.) Merr.) hasil poliploidisasi dengan kolkisin. Scientiae Educatia : Jurnal Sains dan Pendidikan Sains. 5 (2) : 90-98.

Nyffeler, R. and Eggli, U. 2010. Disintegrating Portulacaceae: A new familial classification of the suborder Portulacineae (Caryophyllales) based on molecular and morphological data. J. Taxon. 59 : 227–240.

Ocampo, G. and J. T. Columbus. 2012. Molecular phylogenetics, historical biogeography, and chromosome number evolution of Portulaca (Portulacaceae). Molecular Phylogenetics And Evolution. 63 : 97–112.

Qonitah, F. Z. 2015. Aklimatisasi tanaman anggrek phalaenopsis amabilis hasil perlakuan kolkisin dengan level ploidi yang berbeda. Institut Pertanian Bogor. (Skripsi).

Saraswati, D. R., T. Rahayu, dan A. Hayati. 2017. Kajian pemberian kolkisin dengan metode tetes terhadap profil poliploidi tanaman zaitun (Olea Europaea). E-Jurnal Ilmiah Biosaintropis (Bioscience-Tropic). 2 (2) : 24-29.

Srivastava, A. and A. G. Joshi. 2009.In vitro behaviour of nodal explants of Portulaca Grandiflora under the influence of cytokinins. J.Biology. 753:43–48.

Tel-Zur, N., M. Dudaib, E. Ravehc, dan Y. Mizrahib. 2011. In situ induction of chromosome doubling in Vine Cacti (Cactaceae). J. Scientia Horticulturae. 129 : 570–576.

Thao, N. T. P., K. Ureshino, I. Y. Ozaki, and H. Okubo. 2003. Induction of tetraploids in ornamental alocasia through colchicine and oryzalin treatments. J. Plant Cell, Tissue and Organ Culture. 72 (1) :19-25.

Tuwo, M. dan A. Indrianto. 2016. Improvement of Orchid Vanda Hybrid (Vanda limbata Blume X Vanda tricolor Lindl. var. suavis) by colchicines treatment in vitro. 10 (11) : 83-89.

Wang, X., H. Wang, C. Shi, X. Zhang, K. Duan and J. Luo. 2015. Morphological, cytological and fertility consequences of aspontaneous tetraploid of the diploid pear (Pyrus Pyrifolia Nakai) cultivar ‘Cuiguan’. J. Scientia Horticulturae. 189 : 59–65.

Winaryo, K. A. P., A. N. Sugiharto, dan Ainurrasjid. 2016. Penampilan fenotipik 2 galur jagung (Zea Mays L.) akibat pemberian kolkhisin. J. Produksi Tanaman. 4 (2) : 161 – 168.

Wu Y., F. Yang , X. Zhao and W. Yang. 2011. Identification of tetraploid mutants of Platycodon grandiflorus by colchicine induction. International Journal of Cytology, Cytosystematics and Cytogenetics. 64 (3) : 343-349.

Yanhong, H. E., S. Yalin, R. Zheng, A. Ye, C. Zhe, and B. Manzhu. 2016. Induction of tetraploid male sterile tagetes erecta by colchicine treatment and its application for interspecific hybridization. Horticultural Plant Journal. 2 (5) : 284–292.


Refbacks

  • There are currently no refbacks.


View JOAC Stats

 

ISSN 2597-4386 (media online)

JOAC is published by Department of Agriculture, Faculty of Animal and Agricultural Sciences, Diponegoro University, Indonesia

 

Creative Commons License
JOAC is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.